Evaluation of Silber-Porter Procedure for Determination of Plasma Hydrocortisone

Peterson, Ralph E.; Karrer, Aurora.; Guerra, Serafim L.

doi:10.1021/ac60121a042

Cited by 398 publications

(87 citation statements)

References 15 publications

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“…Ungar, Rosenfeld, Dorfman and Pincus (1955) have demonstrated a depression of steroid synthesis following irradiation of the isolated perfused calf adrenal, which would help explain the earlier observation of Edelmann (1951) that shielding of rat adrenals from whole body irradiation increased their survival rate. The early fall in plasma hydrocortisone concentration, observed in two patients exposed to abdominal field irradiation appeared greater than could be attributed to diurnal decline (Peterson, 1957). It is probably explained by a transitory adrenal depression, and is similar to the findings of Notter and Gemzell (1956) who observed a depression in plasma 17-hydroxycorticosteroid concentration 30 minutes after X-irradiation of the adrenal.…”

Section: Resultssupporting

confidence: 86%

The Effects Of X-Irradiation on Adrenal Function in Man

Hatfield¹,

Shuster²

1958

Br J Cancer

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Section: Resultssupporting

confidence: 86%

The Effects Of X-Irradiation on Adrenal Function in Man

Hatfield¹,

Shuster²

1958

Br J Cancer

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“…scribed previously (1). Cortisone and certain of its metabolites in plasma were determined by a modification (2) of the original phenylhydrazine procedure of Silber and Porter (3). Specificity of this method of assay for plasma hydrocortisone after administration of hydrocortisone was shown to be high, as tested by countercurrent distribution and isotope dilution techniques.…”

Section: Methodsmentioning

confidence: 99%

The Physiological Disposition and Metabolic Fate of Cortisone in Man

Peterson¹,

Pierce²,

Wyngaarden³

et al. 1957

J. Clin. Invest.

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In an earlier paper we described studies on the physiological disposition and metabolic fate of hydrocortisone in man (1). This paper describes similar studies on cortisone. Although contributing very little to the body pool of adrenal cortical steroids, cortisone has been used extensively as a therapeutic agent, and it was therefore of interest to compare its metabolism with that of hydrocortisone. Large doses of cortisone and hydrocortisone were administered intravenously and rates of disappearance from the plasma determined in normal subjects and in patients with liver disease, myxedema, thyrotoxicosis, and rheumatoid arthritis. Trace quantities of cortisone-4-C" and hydrocortisone-4-C" were employed in certain studies. From these studies, information was obtained regarding rate of metabolic transformation, rate of absorption, rates and routes of excretion of cortisone and its metabolites, and, to some extent, the identity and amount of these metabolites. Information was also obtained on the in vivo conversion of cortisone to hydrocortisone. MATERIALS AND METHODSAdministration of steroid. The steroids were dissolved in 25 ml. ethanol and added to 500 ml. of sterile five per cent dextrose in water. This solution was administered intravenously over a period of 10 to 20 minutes. For oral or intramuscular administration the steroids were suspended in saline. Cortisone-4-C" was administered intravenously in trace quantities in a small volume of five per cent ethanol in water over a period of 3 to 5 minutes, or mixed with carrier cortisone and given in 500 ml. of three per cent ethanol in five per cent dextrose in sterile distilled water. Following the administration of the steroids, blood samples were drawn in heparinized syringes every 20 or 30 minutes for 2 hours-or more.Urine samples were collected and preserved by freezing.Methods of assay. The extraction procedures for the steroids in plasma and urine were the same as those de-

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“…The 17-OHCS levels were determined by the method of Peterson, Karrer an(l Guerra (6). Blood from postoperative patients was obtained in the recovery room, usually 30 to 60 minutes after completion of the operation.…”

Section: Ethods and Materialsmentioning

confidence: 99%

Transcortin: A Corticosteroid-Binding Protein of Plasma. Iii. The Effects of Various Steroids *†

Sandberg¹,

Slaunwhite²,

Carter³

1960

J. Clin. Invest.

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In previous publications (1, 2) we have described some of the characteristics of transcortin, a plasma protein (or proteins) with high affinity for cortisol and corticosterone. In addition, we have shown that plasmiia transcortin levels are elevated during pregnancy and following administration of estrogens (1-3). A different interpretation of the findings in these two states has been given by Daughaday and AMariz (4,5). Transcortin levels were substantially lower in the plasma of newborn infants (umnbilical vein 1lood)1 than those found in adult subjects or in older children (2). The purpose of this paper is to present results of further studies on transcortin levels in human sub-*This investigation was supported in part by grants (A-1240-C3) 1lfl,17a,21-trihydroxy-pregnane-3,20-dione; tetrahydrocortisol (H4F) 3a,110,17a,21-tetrahydroxy-pregnane -20-one; 9a -fluorocortisol: 9a-fluoro-1 I,17a,21-trihydroxy-4-pregnene-3,20-dione; cortisone: 17a,21 -dihydroxy-4-pregnene-3,11,20-trione; di - jects under variotus condlitions and the effects of admiiniistered steroi(ls on these levels. MI ETHODS AND MATERIALSThe binding of C'4-cortisol by plasma transcortin and the transcortiin capacity were determined by methods previously described (1, 2). The terms "transcortin binding of C'4-cortisol" and "transcortin capacity" have been defiine(d in a previous publication (2). At this point it may be appropriate to reiterate some important aspects of the methodologies and terms used. Most of the binding experiments wvere performed by dialyzing 10 ml of diluted plasma (1: 5 with physiological saline) against 30 ml of saline containiing approximately 0.3 mg of C14-cortisol. The binding was (letermined with and without the addition of 1 ,ug of cortisol to the saline in order to measure the decrease in the binding caused by the addition of the carrier cortisol. In previously published work (1) we deimioinstrated that un(ler the con(ditions of our assay tlle bindinig of cortisol by plasma was essentially due to transcortini and not to other plasma proteins. Hence, the term "transcortiin bindiing of C'4-cortisol" will refer to the percentage of C'4-cortisol bound by 10 ml of diluted plasma and the term "transcortin capacity" will be used to in(licate the decrease in bindinig caused by the addition of 1 ,ug of cortisol in the dialysis conditions outlined above. It should be pointed out that the "binding capacity" will be inversely related to the decrease in the biniding caused by the addition of 1 ug of cortisol to normal plasma, i.e., the less the decrease in the binding the higher the binding capacity, and vice versa. The measurement of the binding capacity by the method outlined above seems to offer, at the moment, the most practical and sensitive index of traniscortin capacity. It should be realized, however, that this measures the difference between two variables, the level of transcortin and the level of 17-hydroxycorticosteroids (17-OHCS).Lymph was collected from the thoracic ducts of patients with advanced cancer in ice-ch...

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Evaluation of Silber-Porter Procedure for Determination of Plasma Hydrocortisone

Cited by 398 publications

References 15 publications

The Effects Of X-Irradiation on Adrenal Function in Man

The Effects Of X-Irradiation on Adrenal Function in Man

The Physiological Disposition and Metabolic Fate of Cortisone in Man

Transcortin: A Corticosteroid-Binding Protein of Plasma. Iii. The Effects of Various Steroids *†

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