Evaluation of Sialyl-Lactotetra as a Marker for Epithelial Ovarian Tumors

Barone, Angela; Linder, Anna; Mateoiu, Constantina; Larsen, Rasmus; Blixt, Ola; Teneberg, Susann; Sundfeldt, Karin

doi:10.3389/fonc.2020.561888

Cited by 3 publications

(4 citation statements)

References 46 publications

(62 reference statements)

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“…The level of S‐Lc 4 expression is cancer‐related, but the expression of S‐Lc 4 does not increase over time. Altogether, these results suggest that S‐Lc 4 could be a potent marker for serous borderline and type 1 tumors 56 …”

Section: Resultsmentioning

confidence: 69%

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Sialic acids in gynecological cancer development and progression: Impact on diagnosis and treatment

Berghuis

Pijnenborg

Boltje

et al. 2021

Intl Journal of Cancer

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Gynecological cancers are in the top 10 of most common cancers in women. Survival and outcome are strongly related to the stage at diagnosis. Therefore, early diagnosis is essential in reducing morbidity and mortality. The high mortality rate of gynecological cancers can mainly be attributed to ovarian cancer (OC). OC is commonly diagnosed at an advanced stage due to a lack of proper screening tools allowing early detection. Endometrial cancer (EC) on the contrary, is mostly diagnosed at an early stage and has, in general, better outcomes. The incidence of nonendometrioid EC has increased in the last decade, displaying a shared tumor biology with OC and consequently significantly worse outcome. New approaches allowing detection of gynecological cancers in an early stage are therefore desired. Recent studies on cancer biology have shown the relevance of altered glycosylation in the occurrence and progression of cancer. The aberrant expression of sialic acid, a specific carbohydrate terminating glycoproteins and glycolipids on the cell‐surface, is frequently correlated with malignancy. We aimed to determine the current understanding of sialic acid function in different gynecological cancers to identify the gaps in knowledge and its potential use for new diagnostic and therapeutic avenues. Therefore we performed a review on current literature focusing on studies where sialylation was linked to gynecological cancers. The identified studies showed elevated levels of sialic acid in serum, tissue and sialylated antigens in most patients with gynecological cancers, underlining its potential for diagnosis.

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Section: Resultsmentioning

confidence: 69%

“…Altogether, these results suggest that S‐Lc 4 could be a potent marker for serous borderline and type 1 tumors. 56 …”

Section: Resultsmentioning

confidence: 99%

Sialic acids in gynecological cancer development and progression: Impact on diagnosis and treatment

Berghuis

Pijnenborg

Boltje

et al. 2021

Intl Journal of Cancer

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“…Sulfotransferase protein expression levels in serous ovarian tissues were evaluated using TMA with tissue from 323 ovarian tumor specimens (Supplemental Table S4). The construction and scanning of the TMA is described elsewhere (20). The paraffin sections were deparaffinated in xylene and then rehydrated.…”

Section: Methodsmentioning

confidence: 99%

Sulfation of O-glycans on mucin-type proteins from serous ovarian epithelial tumors

Hulthe

Vitiazeva

Mateoiu

et al. 2021

Preprint

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Despite that sulfated O-linked glycans are abundant on ovarian cancer (OC) glycoproteins, their regulation during cancer development and involvement in cancer pathogenesis remain unexplored. We characterized O-glycans carrying sulfation on galactose residues and compared their expression to defined sulfotransferases regulated during OC development. Desialylated sulfated oligosaccharides were released from acidic glycoproteins in the cyst fluid from one patient with a benign serous cyst and one patient with serous OC. Oligosaccharides characterized by LC-MSn were identified as core 1 and core 2 O-glycans up to the size of decamers, and with 1-4 sulfates linked to GlcNAc residues and to C-3 and/or C-6 of Gal. To study the specificity of the potential ovarian sulfotransferases involved, Gal3ST2 (Gal-3S)-, Gal3ST4 (Gal-3S)-, and CHST1 (Gal-6S)-encoding expression plasmids were transfected individually into CHO cells also expressing the P-selectin glycoprotein ligand-1/mouse immunoglobulin G2b (PSGL-1/mIg G2b) fusion protein and the human core 2 transferase (GCNT1). Characterization of the PSGL-1/mIg G2b O-glycans showed that Gal3ST2 preferentially sulfated Gal on the C-6 branch of core 2 structures and Gal3ST4 preferred Gal on the C-3 branch independently if core-1 or-2. CHST1 sulfated Gal residues on both the C-3 (core 1/2) and C-6 branches of core 2 structures. Using serous ovarian tissue micro array, Gal3ST2 was found to be decreased in tissue classified as malignant compared to tissues classified as benign or borderline, with the lowest expression in poorly differentiated malignant tissue. Neither Gal3ST4 nor CHST1 were differentially expressed in benign, borderline or malignant tissue, and there was no correlation between expression level and differentiation stage. The data displays a complex sulfation pattern of O-glycans on OC glycoproteins and that aggressiveness of the cancer is associated with a decreased expression of the Gal3ST2 transferase.

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“…Sulfotransferase protein expression levels in serous ovarian tissues were evaluated using TMA with tissue from 323 ovarian tumor specimens ( supplemental Table S4 ). The construction and scanning of the TMA are described elsewhere ( 21 ). The paraffin sections were deparaffinated in xylene and then rehydrated.…”

Section: Methodsmentioning

confidence: 99%

Sulfation of O-glycans on Mucin-type Proteins From Serous Ovarian Epithelial Tumors

Thomsson

Vitiazeva

Mateoiu

et al. 2021

Molecular & Cellular Proteomics

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Evaluation of Sialyl-Lactotetra as a Marker for Epithelial Ovarian Tumors

Cited by 3 publications

References 46 publications

Sialic acids in gynecological cancer development and progression: Impact on diagnosis and treatment

Sialic acids in gynecological cancer development and progression: Impact on diagnosis and treatment

Sulfation of O-glycans on mucin-type proteins from serous ovarian epithelial tumors

Sulfation of O-glycans on Mucin-type Proteins From Serous Ovarian Epithelial Tumors

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