Evaluation of serum zinc level and plasma SOD activity in senile cataract patients under oxidative stress

Chakraborty, Indranil; Kunti, Sanjoy; Bandyopadhyay, Mausumi; Dasgupta, Anindya; Chattopadhyay, Gopal Deb; Chakraborty, Sandip

doi:10.1007/bf02913326

Cited by 24 publications

(20 citation statements)

References 14 publications

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“…It was suggested that SOD activity was more sensitive to zinc rather than cadmium exposure. Similar results were also reported in other organisms previously (Olin et al 1995;Chakraborty et al 2007). Perhaps it could be explained that zinc was an essential component of copper/zinc SOD, and addition of external zinc was convenient to stabilize copper/zinc SOD and enhance SOD enzyme activity (Kajihara et al 1988;Jing et al 2007).…”

Section: Discussionsupporting

confidence: 84%

Effects of heavy metals on the expression of a zinc-inducible metallothionein-III gene and antioxidant enzyme activities in Crassostrea gigas

Cong

Liu

et al. 2012

Ecotoxicology

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Sequestration by metallothioneins and antioxidant defense are two kinds of important defense mechanisms employed by mollusks to minimize adverse effects caused by heavy metal contaminants in marine environment. In the present study, a novel metallothionein gene, CgMT-III, was cloned from Crassostrea gigas, consisting of eighteen conserved cysteine residues and encoding a MT III-like protein with two tandem b domains. The expression level of CgMT-III transcript induced by zinc was much higher than that induced by cadmium exposure. It suggested that CgMT-III was perhaps mainly involved in homeostatic control of zinc metabolism, which was distinct from previously identified MTs in C. gigas. Among the tested antioxidant enzymes including superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), SOD and GPx showed varying up-regulations in a tissuespecific manner, while CAT activities were inhibited in both gill and hepatopancreas from C. gigas exposed to heavy metals. It can be inferred that CgMT-III was mainly involved in zinc homeostasis, and CgMT-III gene together with CAT enzyme could be potential biomarkers to indicate heavy metal, especially zinc pollution in marine organisms.

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Section: Discussionsupporting

confidence: 84%

Effects of heavy metals on the expression of a zinc-inducible metallothionein-III gene and antioxidant enzyme activities in Crassostrea gigas

Cong

Liu

et al. 2012

Ecotoxicology

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“…10 In our study MDA is the indicator of lipid peroxidation, which is significantly elevated (P<0.001) when compared with controls suggesting that increased MDA and lipid peroxidation may be considered as indicators of senile cataract formation. Similar observations also made by Babizhayer MA et al 11 Donma et al 4 Renu Garg et al 12 Indranil chakraborthy et al 13 Zehra Hashim and Shanshad Zarina. 14 The lipid peroxides are responsible for the formation of cataract and also cataract causes increased lipid peroxidation levels, thus the development of a vicious cycle involving cataract, cell damage, and lipid peroxides is involved in the pathology of the lens.…”

Section: Resultssupporting

confidence: 76%

Lipid Peroxidation a Nd Lipid Profile in Patients With Senile Cataract

Uppu¹,

Gupta²

2015

jemds

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Lipid peroxidation products and biochemical parameters like fasting blood sugar and lipid profile were estimated in serum of 40 senile cataract cases (45-60 years) and 40 persons of age and sex matched healthy controls. Plasma Thiobarbituric acid reactive substances (TBARS) levels [as Malondialdehyde (MDA)] were significantly higher in patients with senile cataract (573±64.4nmol/dl, P<0.001) as compared to healthy controls (378±31.31 nmol/dl). Serum cholesterol, Triglycerides, LDLC, VLDLC levels were increased as compared to controls. But HDLC levels were decreased in senile cataract patients as compared to controls. No significant change was observed in FBS values. The present study shows that the oxidative stress may play an important role in the senile cataract.

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“…SPD ϭ surfactant protein D decreased levels of serum superoxide dismutase 3 in the COPD group compared with the control group and are in agreement with those of Zeng et al, 50 Gavali et al, 51 and Tavilani et al 52 This decreased activity in subjects with COPD probably results from an increase in consumption of antioxidants. 53 We found a superoxide dismutase 3 levels of 244.79 Ϯ 69.12 U/L in the control group (see Table 4), whereas Chakraborty et al 54 reported plasma superoxide dismutase levels of 4.06 Ϯ 0.26 U/mL, KoturStevuljevic et al 55 found plasma superoxide dismutase levels of 153 Ϯ 36 U/L in non-coronary heart disease control subjects, and Comhair et al 56 reported a mean serum superoxide dismutase level of 2.75 U/mL. These differences may be due to differences in measurement methods.…”

Section: Discussionmentioning

confidence: 59%

Diagnostic Utility of Biomarkers in COPD

Ambade¹,

Sontakke

Barthwal³

et al. 2015

Respir Care

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BACKGROUND: COPD will become the third leading cause of death by 2020. There are many situations in which spirometry, the primary tool for diagnosis of COPD, cannot be performed, and thus, the staging and status of these patients cannot be determined. To date, there is no known biochemical marker used for diagnosing COPD. This study aimed to explore the utility of biomarkers for diagnosis of COPD. METHODS: This was an observational study composed of 96 stable subjects with COPD and 96 subjects with normal lung function. Each group contained an equal number of smokers and nonsmokers. Serum levels of superoxide dismutase 3, glutathione peroxidase, catalase, ceruloplasmin ferroxidase activity, C-reactive protein, and surfactant protein D (SPD) were estimated. Ferroxidase activity was estimated by a kinetic method, whereas the other analytes were measured by enzyme-linked immunosorbent assay. The cutoff value, sensitivity and specificity at the cutoff value, and area under the curve for each analyte were determined from receiver operating characteristic curve. RESULTS: Significantly decreased superoxide dismutase 3 and increased ferroxidase activity, SPD, glutathione peroxidase, and C-reactive protein levels were found in subjects with COPD. For all subjects and nonsmoking subjects with COPD, the area under the curve was highest for ferroxidase activity, followed by glutathione peroxidase, SPD, and C-reactive protein, with a sensitivity and specificity of > 73%. For smoking subjects with COPD, the area under the curve was highest for SPD, followed by glutathione peroxidase, ferroxidase activity, and C-reactive protein, with a sensitivity and specificity > 67%. Some combinations of markers were found to give either a sensitivity or specificity of > 95%, which can be utilized to rule in and rule out COPD. CONCLUSIONS: Biomarkers can be reliably utilized in the diagnosis of COPD. Of all the markers, SPD appears to be the most promising in smokers, whereas ferroxidase activity shows promise in nonsmokers. To rule out COPD, ferroxidase activity or glutathione peroxidase can be potentially useful, whereas to rule in COPD, ferroxidase activity and glutathione peroxidase appear to be the most promising combination in both nonsmoking and smoking subjects.

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Evaluation of serum zinc level and plasma SOD activity in senile cataract patients under oxidative stress

Abstract: ABSTRACT

Cited by 24 publications

References 14 publications

Effects of heavy metals on the expression of a zinc-inducible metallothionein-III gene and antioxidant enzyme activities in Crassostrea gigas

Effects of heavy metals on the expression of a zinc-inducible metallothionein-III gene and antioxidant enzyme activities in Crassostrea gigas

Lipid Peroxidation a Nd Lipid Profile in Patients With Senile Cataract

Diagnostic Utility of Biomarkers in COPD

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