Evaluation of serological assays based on a novel excreted antigen preparation for the diagnosis of Cutaneous Leishmaniasis in Panama

Romero, Luz; Paz, Hector Marino Pérez; Ortega-Barrı́a, Eduardo; Bayard, Vicente; Hochberg, Lisa P.; Collins, K; Chan, Adeline; Ryan, Jeffrey R.

doi:10.1016/j.mimet.2004.02.008

Cited by 16 publications

(15 citation statements)

References 23 publications

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“…Indeed, our findings of a 37.5% positivity of ESA with CL and 13.3% with the MCL cases (Table 1) do not agree with those of other authors who have reported 80-92.3% and 71% positive rates when CL samples were assayed with ESA isolated from L. (L.) mexicana and L. (V.) braziliensis, respectively. 13,15 In contrast, similar positivity rates (62.5% for CL and 73.3% for MCL) were observed in our study when Leishmania spp. PRO was used (Table 1).…”

Section: Discussionsupporting

confidence: 87%

“…have been a target of interest for decades and have been identified as potential candidates for vaccines, 32 they have not been extensively explored for the diagnosis of leishmaniasis. 5,6,12,13,15 In this report, we present new data suggesting that ESAs isolated from three different species of Leishmania (from the most prevalent species in the Americas) L.…”

Section: Discussionmentioning

confidence: 79%

“…conditioned medium has been used as a potential source of antigens for immunodiagnosis in the detection of cases of human VL, 5,6,12,13 canine leishmaniasis, 12,14 and cutaneous leishmaniasis (CL). 13,15 Although serological tests, such as the direct agglutination test, immunofluorescence test, indirect hemagglutination assay, enzyme-linked immunosorbent assay (ELISA), and Western blotting have been found to display high levels of sensitivity, the specificity indices were not significant. 1,2 The reason for this result may be because the antigens used in immunodiagnostic tests are usually derived from crude promastigotes of different Leishmania species, which express a variety of complex molecules that are common to other microorganisms, such as Trypanosoma, Plasmodium, and Mycobacterium, [16][17][18][19][20] or proteins that can react non-specifically.…”

Section: Introductionmentioning

confidence: 99%

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The Efficacy of L. (L.) chagasi Excreted-Secreted Antigens (ESAs) for Visceral Leishmaniasis Diagnosis Is Due To Low Levels of Cross-Reactivity

Pinedo-Cancino¹,

Kesper²,

Barbiéri³

et al. 2013

The American Society of Tropical Medicine and Hygiene

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Abstract. The analysis of promastigote excreted-secreted antigen (ESA) reactivity with 53 visceral leishmaniasis (VL) cases showed that each sample reacted regardless of the antigen or the Leishmania species used in enzyme-linked immunosorbent assay (ELISA) displayed 100% positivity with the L. (L.) chagasi ESA-blot recognizing bands of molecular weight ranging from 26.5 to 31.5 kDa. The analysis of 160 non-visceral cases showed that 5% of the samples crossreacted with the L. (L.) chagasi ESA-ELISA and 9.4% reacted with the ESA isolated from L. (L.) amazonensis and L. (V.) braziliensis, whereas a high cross-reaction ranging from 24.4% to 25% was observed with total crude promastigote antigens (PRO-ELISA). The ESA-blot of L. (L.) chagasi tested with non-visceral sera samples showed a cross-reaction with 8.8% of cases; most of these cases represented tegumentary leishmaniasis and only one acute chagasic case. These data lead us to recommend the use of ESA as an alternative antigen in VL diagnosis.

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Section: Discussionsupporting

confidence: 87%

Section: Discussionmentioning

confidence: 79%

Section: Introductionmentioning

confidence: 99%

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The Efficacy of L. (L.) chagasi Excreted-Secreted Antigens (ESAs) for Visceral Leishmaniasis Diagnosis Is Due To Low Levels of Cross-Reactivity

Pinedo-Cancino¹,

Kesper²,

Barbiéri³

et al. 2013

The American Society of Tropical Medicine and Hygiene

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“…The use of these serologic tools in diagnosis of CL has also been evaluated in a few of studies [23–25]. Nevertheless, no serologic test is yet available for routine diagnosis of CL.…”

Section: Discussionmentioning

confidence: 99%

“…These findings are consistent with the findings of our study. Romero et al reported a sensitivity of 89% for an ELISA system with antigen of Leishmania mexicana and sensitivity of 71% with antigen of Leishmania braziliensis [25]. Jensen et al reported an ELISA test with a sensitivity of 67% in 33 L. major infected CL patients, by using a sequence specific peptide antigen [24].…”

Section: Discussionmentioning

confidence: 99%

Performance of an ELISA and Indirect Immunofluorescence Assay in Serological Diagnosis of Zoonotic Cutaneous Leishmaniasis in Iran

Sarkari

Ashrafmansouri

Hatam

et al. 2014

Interdisciplinary Perspectives on Infectious Diseases

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Serological assays have been extensively evaluated for diagnosis of visceral leishmaniasis (VL) and considered as a routine method for diagnosis of VL while these methods are not properly evaluated for diagnosis of cutaneous leishmaniasis (CL). This study aimed to assess the performance of indirect immunofluorescent-antibody test (IFA) and enzyme-linked immunosorbent assay (ELISA) for serodiagnosis of cutaneous leishmaniasis in Iran. Sixty-one sera samples from parasitologically confirmed CL patients and 50 sera from healthy controls along with 50 sera from non-CL patients were collected. Antigen was prepared from promastigotes and amastigotes of Leishmania major. IFA was used to detect anti-Leishmania IgG while ELISA was used to detect anti-Leishmania IgM, total IgG, or IgG subclasses (IgG1 and 4). ELISA, for detection of total IgG and IgM, showed sensitivity of 83.6% and 84.7% and specificity of 62.7% and 54.6%, respectively. Sensitivity and specificity of ELISA for detecting IgG1 and IgG4 were 64%, 75% and 85%, 49%, respectively. Sensitivity and specificity of IFA were 91.6% and 81%. Conclusion. Findings of this study demonstrated that serological test, especially IFA, can be used for proper diagnosis of CL.

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Leishmaniasis diagnosis: an update on the use of parasitological, immunological and molecular methods

2020

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Diagnosis of leishmaniasis has always been a major challenge as its clinical features resemble some other commonly occurring diseases such as tuberculosis, typhoid, and malaria. Reliable laboratory methods become important for differential diagnosis. Demonstration of the parasites in stained preparations of bone marrow and splenic aspirates being risky and invasive is still the gold standard for diagnosis. Serological tests utilizing rapid immunochromatographic formats or rK39 in enzyme linked immune sorbent assay, immunoblotting, direct agglutination test have complications related to high proportions of positive asymptomatic individuals and the inability to diagnose a relapse. Among the molecular techniques, polymerase chain reaction is the most commonly used technique that is successfully implied for diagnosis. This review provides updated information on the recent developments in the field of diagnosis in leishmaniasis, various methods utilized with their advantages and limitations.

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Evaluation of serological assays based on a novel excreted antigen preparation for the diagnosis of Cutaneous Leishmaniasis in Panama

Cited by 16 publications

References 23 publications

The Efficacy of L. (L.) chagasi Excreted-Secreted Antigens (ESAs) for Visceral Leishmaniasis Diagnosis Is Due To Low Levels of Cross-Reactivity

The Efficacy of L. (L.) chagasi Excreted-Secreted Antigens (ESAs) for Visceral Leishmaniasis Diagnosis Is Due To Low Levels of Cross-Reactivity

Performance of an ELISA and Indirect Immunofluorescence Assay in Serological Diagnosis of Zoonotic Cutaneous Leishmaniasis in Iran

Leishmaniasis diagnosis: an update on the use of parasitological, immunological and molecular methods

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