Evaluation of Sera from Patients with Lyme Disease in the Fluorescent Treponemal Antibody-absorption Test for Syphilis

Hunter, Elizabeth F.; Russell, Harold; Farshy, Carol E.; Sampson, Jacquelyn S.; Larsen, Sandra A.

doi:10.1097/00007435-198610000-00005

Cited by 75 publications

(29 citation statements)

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“…Another factor that lowers the titer and is considered diagnostic is whether a "sorbent" material is used. In most reports the adsorbing material was derived from Treponema phagedenis biotype Reiter cells (2, 54,89,126,128,130,177,178). While the adsorption step reduces antibody titers to both B. burgdorferi and T. phagedenis in Lyme disease patients, the magnitude of the drop is greater against T. phagedenis.…”

Section: Differential Diagnosismentioning

confidence: 99%

“…On the other hand, European strains are more heterogeneous in the types of major outer membrane proteins they possess (Fig. 3) (89,116,117). Lyme borreliosis patients occasionally have reactive fluorescent treponemal antibodyabsorption and treponemal agglutination tests for syphilis (89,117,128); in these as well as treponemal antibodynegative cases, reagin antibody-assays, such as the Venereal Disease Research Laboratory and rapid plasma reagin tests, are negative.…”

Section: Differential Diagnosismentioning

confidence: 99%

“…3) (89,116,117). Lyme borreliosis patients occasionally have reactive fluorescent treponemal antibodyabsorption and treponemal agglutination tests for syphilis (89,117,128); in these as well as treponemal antibodynegative cases, reagin antibody-assays, such as the Venereal Disease Research Laboratory and rapid plasma reagin tests, are negative. As demonstrated by IFA, syphilis, yaws, and pinta patients have had high titers of antibodies that react with B. burgdorferi (14,117,126).…”

Section: Differential Diagnosismentioning

confidence: 99%

See 2 more Smart Citations

Laboratory aspects of Lyme borreliosis

Barbour

1988

Clin Microbiol Rev

129

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Lyme borreliosis (Lyme disease), a common tick-borne disorder of people and domestic animals in North America and Europe, is caused by the spirochete Borrelia burgdorferi. Following the discovery and initial propagation of this agent in 1981 came revelations that other tick-associated infectious disorders are but different forms of Lyme borreliosis. A challenge for the clinician and microbiology laboratory is confirmation that a skin rash, a chronic meningitis, an episode of myocarditis, or an arthritic joint is the consequence of B. burgdorferi infection. The diagnosis of Lyme borreliosis may be established by (i) directly observing the spirochete in host fluid or tissue, (ii) recovering the etiologic spirochete from the patient in culture medium or indirectly through inoculation of laboratory animals, or (iii) carrying out serologic tests with the patient's serum or cerebrospinal fluid. The last method, while lacking in discriminatory power, is the most efficacious diagnostic assay for most laboratories at present.

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Section: Differential Diagnosismentioning

confidence: 99%

Section: Differential Diagnosismentioning

confidence: 99%

Section: Differential Diagnosismentioning

confidence: 99%

See 1 more Smart Citation

Laboratory aspects of Lyme borreliosis

Barbour

1988

Clin Microbiol Rev

129

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“…None of the 38 serum samples with false-positive results in other syphilis tests or the 20 possible cross-reacting serum samples from patients with confirmed Lyme borreliosis were reactive in the PaGIA. It is known that cross-reacting antibodies can cause false-positive results in the FTA-ABS test (4). However, all 20 serum samples from patients with confirmed Lyme borreliosis were nonreactive in the PaGIA.…”

mentioning

confidence: 95%

Evaluation of a New Particle Gel Immunoassay for Determination of Antibodies against Treponema pallidum

Schmidt

2004

J Clin Microbiol

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A new particle gel immunoassay (DiaMed AG, Cressier sur Morat, Switzerland) with three recombinant Treponema pallidum antigens was evaluated with serum samples from patients with syphilis (n ‫؍‬ 124) and patients without syphilis (n ‫؍‬ 490). It proved to be a simple, rapid (20 min), and useful test with sensitivity, specificity, and positive and negative predictive values of 91.9, 99.8, 99.2, and 98%, respectively.

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“…However, this confirmatory test can also lead to false-positive or falsenegative results if the fluorescence intensity is misinterpreted or if the testing equipment is suboptimal (7). False-positive results have been reported for patients with other spirochetal infections or immunological disorders (e.g., systemic lupus erythematosis, rheumatoid factors, antinuclear and anti-DNA antibodies or cross-reacting antibodies such as those from Borrelia burgdorferi) (4, 7-9, 11, 12, 14).…”

mentioning

confidence: 99%