Evaluation of “sequence-tagged-site” PCR products as molecular markers in wheat

Talbert, L. E.; Blake, N. K.; Chee, Peng W.; Blake, T. K.; Magyar, G. M.

doi:10.1007/bf00221130

Cited by 112 publications

(46 citation statements)

References 16 publications

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“…Some advantages of gliadin alleles for wheat genotype identification over up-to-date molecular markers have been discussed (Metakovsky et al, 1997). Although located on only two chromosomes of seven homologous groups, gliadin genes (Payne et al, 1982) show many more multiple alleles than other wheat genes: more than 30 allelic variants were described and catalogued for some Gli loci (Metakovsky et al, 1991), while only a few alleles were found for the most polymorphic DNA markers (Talbert et al, 1994;Röder et al, 1995). Therefore, using gliadin alleles enables investigation of wheat cultivars to reveal much more intraspecific genetic polymorphism.…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity for gliadin patterns of durum wheat landraces in the Northwest of Iran and Azerbaijan

Zaefizadeh

Jamaati-e-Somarin

Ojaghi

et al. 2010

Pesq. agropec. bras.

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-The objective of this study was to identify gliadin band patterns and the extent of genetic diversity in durum wheat genotypes from Northwestern Iran and the Republic of Azerbaijan. Gliadins from 46 landraces and four cultivars were evaluated through acid PAGE analyses. Sixty-six polymorphic bands and 81 patterns were identified. Twenty-four different motility bands and 22 patterns were found in the ω gliadin region with 14 polymorph bands and 20 patterns for α and γ gliadins, and 14 bands and 19 different patterns for β gliadins. The combination of these patterns generated 38 and 39 combinations for Gli-1 and Gli-2 loci, respectively. The genetic diversity index (H) was higher for α gliadins (0.924), followed by ω and γ gliadins (0.899 and 0.878, respectively), and for β gliadin patterns (0.866). Extensive polymorphism (H = 0.875) was observed in four gliadin pattern regions, with higher genetic diversity in the Iranian landraces than in the Azerbaijani ones. Each genotype had special identifying patterns in the gliadin acid PAGE analysis, and cluster analysis based on Jaccard's similarity coefficients formed six groups. Gliadin has a simple, repeatable and economic analysis, and can be used in genetic studies.

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Section: Introductionmentioning

confidence: 99%

Genetic diversity for gliadin patterns of durum wheat landraces in the Northwest of Iran and Azerbaijan

Zaefizadeh

Jamaati-e-Somarin

Ojaghi

et al. 2010

Pesq. agropec. bras.

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“…The A1 locus was amplified by PCR according to Talbert et al (1994) with slight modifications. PCR amplification was performed in a reaction mixture of 50 μl containing 1.25 U of Taq polymerase (AmpliTaq Gold, Applied Biosystems, Foster City, CA), 0.5 μM of each primer, 100 ng of total DNA, 0.2 mM of each dNTP, 1.5 mM of MgCl 2 and reaction buffer using a GeneAmp PCR 9700 (Applied Biosystems).…”

Section: Pcr and Sequencingmentioning

confidence: 99%

“…The PCR profile consisted of a pre-denaturation step at 94°C for 10 min, followed by 32 cycles of denaturation at 94°C for 1 min, annealing at 50°C for 1 min and extension at 72°C for 1.2 min, with a final post-extension incubation step at 72°C for 1.2 min. The primer sequences used for PCR amplification were as follows: A1-L: 5′-CAACAGAGATATTGC CGTAG-3′ and A1-R: AAGATTGTCAACAAGTGCC-3′ according to Talbert et al (1994). Amplified DNA fragments were purified using PureLink PCR Purification Kit (Invitrogen Corp., Carlsbad, CA) and subjected to direct DNA sequencing.…”

Section: Pcr and Sequencingmentioning

confidence: 99%

Comparative nucleotide sequence analysis of the D genome-specific sequence-tagged-site locus A1 in Triticum aestivum and its implication for the origin of subspecies sphaerococcum

et al. 2011

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Common wheat (Triticum aestivum) evolved through hybridization between cultivated tetraploid emmer wheat (T. turgidum), which has A and B genomes, and the wild diploid species, Aegilops tauschii, which has the D genome. Although the evolution of common wheat is generally understood, specific details remain unclear. For example, the phylogenetic relationships and origins of the six wheat subspecies (ssp. spelta, macha, vavilovi, aestivum, compactum, and sphaerococcum) have not yet been thoroughly resolved. To clarify the origin of ssp. sphaerococcum, we employed comparative sequence analysis of the D genome-specific sequence-tagged-site (STS) locus A1 in common wheat accessions, including sphaerococcum. Only the two known alleles, type A and type B were found among the accessions. Of the two sphaerococcum accessions, both possessed the type A allele. Four aestivum accessions also possessed the type A allele, while the remaining three accessions possessed the type B allele. Conversely, the accessions of the four remaining subspecies possessed the type B allele. Since sphaerococcum has morphological traits that differ from aestivum and which are pleiotropically regulated by a single recessive gene designated s, sphaerococcum most likely originated from aestivum, with the type A allele at the A1 locus arising through a spontaneous mutation at the s locus.

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“…최근 유럽을 중심으로 벼 (Komori and Nitta, 2004;Sun et al, 2009), 오이 (Bernet et al, 2003), 배추 (Kwon et al, 2003), 밀과 토마토 (Cooke et al, 2003;Noil et al, 2008), 고추 (Lefebvre et al, 2001 (Bang et al, 2010;Jo et al, 2013a), 콩 (Hwang et al, 2012), 보리 (Talbert et al, 1994;Blake et al, 1996;Erpelding et al, 1996), 무궁화 (Lee et al, 2002) …”

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A Rapid Identification of Korean Ginseng Cultivar, Cheonryang, using Specific DNA Markers

Jo¹,

Kim²,

Kim³

et al. 2014

Korean Journal of Medicinal Crop Science

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: This study describes the efficient method for the discrimination of 'Cheonryang' in Panax ginseng Meyer using a STS primer. A total of 208 STS primers were applied to polymerase chain reaction (PCR) amplification for discriminating Korean ginseng cultivars. Co-dominant polymorphic band patterns were generated with two primers, MFGp 0019, MFGp 0248, and successful identification of 'Cheonryang' was achieved from out of 11 Korean ginseng cultivars. Two different sizes of DNA band patterns were detected with MFGp 0019 primer. Ten Korean ginseng cultivars shared the same size of amplified DNAs (389 bp), but 'Cheonryang' showed a different size. Thus 'Cheonryang' can be efficiently distinguished from the other ten ginseng cultivars by using the MFGp 0019 primer. In the case of MFGp 0248, two different sizes of DNA band patterns were detected in the eleven ginseng cultivars. Same sized amplified DNA bands (307 bp) were shown in five cultivars (Chunpoong, Gopoong, Kumpoong, Cheongsun, Sunhyang) and 254 bp sized DNA bands were identified in the other 6 cultivars (Yunpoong, Sunpoong, Sunun, Sunone, Cheonryang, K-1). In conclusion, the two STS primers, MFGp 0019, and MFGp 0248, provide a rapid and reliable method for the specific identification of 'Cheonryang' cultivar from a large number of samples.

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Evaluation of “sequence-tagged-site” PCR products as molecular markers in wheat

Cited by 112 publications

References 16 publications

Genetic diversity for gliadin patterns of durum wheat landraces in the Northwest of Iran and Azerbaijan

Genetic diversity for gliadin patterns of durum wheat landraces in the Northwest of Iran and Azerbaijan

Comparative nucleotide sequence analysis of the D genome-specific sequence-tagged-site locus A1 in Triticum aestivum and its implication for the origin of subspecies sphaerococcum

A Rapid Identification of Korean Ginseng Cultivar, Cheonryang, using Specific DNA Markers

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