Evaluation of Semen

Madill, Scott; Kustritz, Margaret V. Root

doi:10.1002/9781119380559.ch41

Cited by 3 publications

(3 citation statements)

References 175 publications

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“…Data analysis of methods for the estimation of sperm concentration were consistent because the guaranteed value was closer than the sperm concentration mean. Previous studies that compared the accuracy of sperm concentration using three methods, hemocytometer, spectrophotometer and flow cytometry, found that when defining threshold values, the k80 value could be closer to the mean values in all three methods, indicating that the use of this measure can improve the accuracy of sperm concentration estimation across different methods [36][37][38].…”

Section: Discussionmentioning

confidence: 90%

Are There Differences between Methods Used for the Objective Estimation of Boar Sperm Concentration and Motility?

Sevilla,

Soler,

Araya-Zúñiga

et al. 2023

Animals

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Artificial insemination in the swine industry, as in other species, demands adequate semen handling and accurate evaluation for the preparation of seminal doses. Sperm concentration and motility estimates are part of the semen evaluation process and are considered important for maximizing the yield of doses for insemination. In this study, methods were examined for their accuracy in the estimation of boar sperm concentration and motility. Assessments of sperm concentration were carried out using iSperm®, ISAS® v1, Open CASA v2, and the Accuread® photometer. Analyses of sperm motility were performed with iSperm®, ISAS® v1, and Open CASA v2 systems. In this study, boar semen samples were collected from 10 healthy males from two genetic lines. There were no relevant differences between sire lines when sperm concentration was assessed. A Bayesian analysis was applied to the four methods used to assess sperm concentration to examine whether there are relevant differences between them. Results suggested differences in the four methods, with a probability of relevance (PR) of 0.86–1.00. The iSperm® method revealed higher concentration values within the highest posterior density region at 95% confidence interval (HPD95%) = 167.0, 224.2 M/mL, whereas Open CASA v2 showed the lowest values, with HPD95% = 99.3, 155.9 M/mL. The iSperm® demonstrated higher reliability in measuring sperm concentration compared to other methods or devices within the given range of confidence. ANOVAs revealed relevant differences in the three methods of motility estimation. Overall, differences in boar sperm concentration and motility estimates were found using various methods, but further studies are needed for better characterization of these differences.

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Section: Discussionmentioning

confidence: 90%

Are There Differences between Methods Used for the Objective Estimation of Boar Sperm Concentration and Motility?

Sevilla,

Soler,

Araya-Zúñiga

et al. 2023

Animals

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“…Sperm count was calculated using Czubaszek et al's [23] method, and sperm morphology was assessed using aniline blue staining. Epididymal volume was identified by displaced volume past the epididymis [24]. DSP was measured in adult rats by homogenizing testes in 0.9% sodium chloride solution and counting the resulting aliquot under a 40× objective light microscope [25].…”

Section: Sperm Characterizationmentioning

confidence: 99%

Diosmin protects the testicles from doxorubicin-induced damage by increasing steroidogenesis and suppressing oxido-inflammation and apoptotic mediators

Oyovwi

2024

Int J Biochem Mol Biol

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Background: Cancer chemotherapy with doxorubicin (DOX) has been linked to serious testicular damage and spermatotoxicity due to the induction of oxidative stress, inflammation, and apoptosis. Thus, the current study was carried out to assess the potential ameliorative impact of diosmin, an antioxidant drug, against DOX-mediated spermatoxicity and testicular injury in rats. Material and Methods: In the experimental protocol, rats were grouped into 4: Group 1 received vehicle and saline for 8 weeks while group 2 received diosmin and saline concomitantly for 8 weeks. Group 3 was given 3 mg/kg intraperitoneal DOX once every 7 days for 8 weeks. Group 4 was given 40 mg/ kg of diosmin orally for 56 days followed by DOX diosmin administration after one hour. After 56 days of treatment, sperm quality, hormonal testing, biochemical parameters, and histological alterations in the testes were evaluated. Results: DOX-induced reduce spermatogenic function, testicular 3-and 17β-Hydroxysteroid dehydrogenases, and serum follicle stimulating hormone, luteinizing hormone, and testosterone. It also enhanced inflammation, testicular oxidative damage, and apoptosis. The histopathologic examinations corroborated the biochemical results obtained. Significantly, diosmin treatment reduced DOX-induced injury, as evidenced by restored testicular architecture, increased steroidogenesis, preservation of spermatogenesis, suppression of oxide-inflammatory response, and apoptosis. Conclusion: It was found that through diosmin antioxidant, anti-apoptotic, and anti-oxido-inflammatory it presents a possible therapeutic alternative for protecting testicular tissue against DOX's harmful effects.

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“…Bali bulls sperm were collected twice weekly at 8:00 a.m. using an artificial vagina (Kruuse, model 340284, Denmark) [10]. The volume of semen was determined directly using a scaled vial [11], and the pH was determined using pH indicator paper.…”

Section: Semen Collection and Evaluation Proceduresmentioning

confidence: 99%

Seasonal effects on the semen production of Bali bulls (Bos javanicus) in West Nusa Tenggara's Lelede regional artificial insemination center

Maulana¹,

Anwar²,

Volkandari³

et al. 2022

Livest. Anim. Res.

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Objective: The purpose of this research is to determine the effect of the rainy and dry seasons on the sperm qualities and frozen sperm production of Bali bulls at the Lelede Regional Artificial Insemination Center (RAIC) in West Nusa Tenggara.Methods: A total of 1226 ejaculates were examined from five Bali bulls during a 24-month period (November 2018–October 2019), which was classified into rainy (November–April, 658 ejaculates) and dry (May–October, 568 ejaculates) seasons. Semen volume (mL), sperm velocity (1-4), motility (%), concentration (x106 cell/ml), pre-freezing motility (%), post-freezing motility (%), recovery rate (%), and total frozen semen production were examined as sperm quality measures.Results: The results indicated that during the rainy season, ejaculates and processed sperm were more abundant than during the dry season. Additionally, the features of discarded fresh semen in the volume parameter were significantly (P<0.05) larger during the dry season than during the rainy season. The pattern in semen output rises during the dry season and decreasing during the rainy season.Conclusions: It was concluded that the rainy season results in a higher number of ejaculates and total sperm production than the dry season. Meanwhile, no differences in sperm characteristics exist between the two seasons.

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Evaluation of Semen

Cited by 3 publications

References 175 publications

Are There Differences between Methods Used for the Objective Estimation of Boar Sperm Concentration and Motility?

Are There Differences between Methods Used for the Objective Estimation of Boar Sperm Concentration and Motility?

Diosmin protects the testicles from doxorubicin-induced damage by increasing steroidogenesis and suppressing oxido-inflammation and apoptotic mediators

Seasonal effects on the semen production of Bali bulls (Bos javanicus) in West Nusa Tenggara's Lelede regional artificial insemination center

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