Evaluation of self-purifying power of cyanobacteria Pseudanabaena galeata: case of dairy factory effluents

Abstract

This study aims to evaluate the treatment of wastewater from dairy plants using the self-purifying power of filamentous cyanobacterium Pseudanabaena galeata. The performance and the reliability of using cyanobacteria as wastewater treatment technique were also assessed based on total bacterial flora, total nitrogen (NK), chemical oxygen demand (COD), and total organic carbon (TOC). The results obtained show promising treatment of wastewater if 25% and 50% dilutions were applied. Thus, respectively, for 25 and 50% dilutions, the reduction of the total bacterial flora was 67 and 45%, NK removal was close to 92 and 77%, the COD decreased by 77% and 65%, and the TOC decreased by 78 and 77%. The final concentration of cyanobacteria biomass increased from 15.72 to 585 mg/l and 475 mg/l, respectively, for 25 and 50% dilutions. These results suggest that cyanobacteria (Pseudanabaena galeata) may play an effective role in the biological treatment of wastewater.

Wastewater from maize lime‐cooking as growth media for alkaliphilic microalgae–cyanobacteria consortium to reduce chemical oxygen demand and produce biomass with high protein content

“…Furthermore, Nannochloropsis salina achieved a stationary phase at 7 days with a modified f/2 medium at 250 μmol/m 2 s (Kang et al ., 2015). On the other hand, in studies performed in Pseudanabaena galeata , the stationary phase was reached at day 9 using 25% and 50% of liquid wastewater from a dairy factory as a culture medium illuminated by fluorescent white lamps with 16/8 h light/dark cycles (Ouhsassi et al ., 2020). For the experiments N, NB and NE, the stationary phase was reached between day 4 and 6.…”

“…In similar studies with Nannochloropsis sp ., the removal efficiency ranged between 90% and 50% for oilfield‐produced water depending on the type of wastewater and the adaptable capacities of the strains (Ammar et al ., 2018). Likewise, studies in Pseudanabaena galeata showed a decrease of 92% and 77% of COD using 5% and 50% of dilution of wastewater from dairy plants (Ouhsassi et al ., 2020). Finally, López‐Pacheco et al .…”

“…In similar studies with Nannochloropsis sp., the removal efficiency ranged between 90% and 50% for oilfield-produced water depending on the type of wastewater and the adaptable capacities of the strains (Ammar et al, 2018). Likewise, studies in Pseudanabaena galeata showed a decrease of 92% and 77% of COD using 5% and 50% of dilution of wastewater from dairy plants (Ouhsassi et al, 2020). Finally, L ópez-Pacheco et al (2019) conducted a study with A. maxima and C. vulgaris in which nejayote was mixed with swine wastewater in a proportion of 10% and 90%, respectively, achieving a COD reduction of 96% after ten fermentation days.…”

Cyanobacterial Biomass Produced in the Wastewater of the Dairy Industry and Its Evaluation in Anaerobic Co-Digestion with Cattle Manure for Enhanced Methane Production

“…The use of microalgae and cyanobacteria for the removal of N and P in agro-industrial wastewater is an area of permanent study. Therefore, Ouhsassi et al [73] evaluated the treatment of wastewater from dairy plants with the cyanobacterium Pseudanabaena galeata Böcher 1949, cultivated the microorganisms with different concentrations of wastewater (25,50, and 75% v/v), with a final concentration of TN of 1.1, 2.2 and 3.2 mM, respectively. The highest RE was with the concentration of TN 1.1 mM, with 86.44%.…”

Coupling dairy wastewaters for nutritional balancing and water recycling: sustainable heterologous 2-phenylethanol production by engineered cyanobacteria

“…On the other hand, cyanobacteria, which constitute a group of ancient ubiquitous phototrophic bacteria (Abed et al, 2009;Garcia-Pichel, 2009), are resistant to extreme conditions of temperature, pH, heavy metals, and high salinity and, thus, are suitable for bioremediation (Gaurav et al, 2018;Ahmad, 2022;Lakmali et al, 2022). Although cyanobacteria have the ability to successfully remove nitrogen and phosphate from wastewaters (Lincoln et al, 1996;Kumar et al, 2011;El-Sheekh et al, 2011;Jitha and Madhu, 2016;Kabariya and Ramani, 2016;Ouhsassi et al, 2020;Álvarez et al, 2020;Ahmad, 2022), they have been mainly investigated for the production of high-value compounds, both naturally produced and metabolically engineered, thanks to their ease of genetic manipulation. These compounds can be extruded or derivatives of biomass, such as pigments (Kabariya and Ramani, 2018;Menin et al, 2019;Arashiro et al, 2020;Thevarajah et al, 2023).…”