Evaluation of seed associated endophytic bacteria from tolerant chilli cv. Firingi Jolokia for their biocontrol potential against bacterial wilt disease

Dowarah, Bhaskar; Agarwal, Heena; Krishnatreya, Debasish B.; Sharma, Pankaj; Kalita, Nilamjyoti; Agarwala, Niraj

doi:10.1016/j.micres.2021.126751

Cited by 29 publications

(17 citation statements)

References 63 publications

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“…Previous studies indicate that many beneficial endophytes Bacillus ( B. subtilis , B. amyloliquefaciens , and B. velezensis ), exhibit several disease suppression mechanisms and promote plant growth, including preventing vascular pathogens from colonizing ecological niches, biocontrol attributes [the production of siderophores, hydrolytic enzymes, hydrogen cyanide (HCN), and antibiotics], broad-spectrum antibiotics, PGP traits (IAA production, phosphate solubilization, potassium solubilization, nitrogen fixation, and ACC deaminase), and promoting induced systemic resistance ( Penrose and Glick, 2003 ; Ahmad et al, 2008 ; Senthilkumar et al, 2009 ; Kumar et al, 2012 ; Vacheron et al, 2013 ; Eljounaidi et al, 2016 ; Dowarah et al, 2021 ). The endogenous B. licheniformis XNRB-3 isolated in this study also has several of the aforementioned PGP properties and antagonistic traits.…”

Section: Discussionmentioning

confidence: 99%

The Phlorizin-Degrading Bacillus licheniformis XNRB-3 Mediates Soil Microorganisms to Alleviate Apple Replant Disease

et al. 2022

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In this study, an endophytic phlorizin-degrading Bacillus licheniformis XNRB-3 was isolated from the root tissue of healthy apple trees, and its control effect on apple replant disease (ARD) and how it alleviates the pathogen pressure via changes in soil microbiomes were studied. The addition of strain XNRB-3 in Fusarium infested soils significantly reduced the number of pathogens in the soil, thus resulting in a lower disease incidence, and the relative control effect on Fusarium oxysporum reached the highest of 66.11%. The fermentation broth can also protect the roots of the plants from Fusarium oxysporum, Fusarium moniliforme, Fusarium proliferatum, and Fusarium solani infection. These antagonistic effects were further validated using an in vitro assay in which the pathogen control was related to growth and spore germination inhibition via directly secreted antimicrobial substances and indirectly affecting the growth of pathogens. The secreted antimicrobial substances were identified using gas chromatography-mass spectrometry (GC-MS) technology. Among them, alpha-bisabolol and 2,4-di-tert-butylphenol had significant inhibitory effects on many planted pathogenic fungi. Butanedioic acid, monomethyl ester, and dibutyl phthalate promoted root development of Arabidopsis plants. Strain XNRB-3 has multifarious plant growth promoting traits and antagonistic potential. In pot and field experiments, the addition of strain XNRB-3 significantly promoted the growth of plants, and the activity of enzymes related to disease resistance [superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT)] was also significantly enhanced. It also reduced the abundance of four species of Fusarium and the content of phenolic acids in the rhizosphere soil, improved soil microbial community structure and nutritional conditions, and increased soil microbial diversity and activity, as well as the soil enzyme activity. The above results indicated that B. licheniformis XNRB-3 could be developed into a promising biocontrol and plant-growth-promoting agent.

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Section: Discussionmentioning

confidence: 99%

The Phlorizin-Degrading Bacillus licheniformis XNRB-3 Mediates Soil Microorganisms to Alleviate Apple Replant Disease

et al. 2022

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“…Seeds may indeed host highly specialized microbial communities determined by environmental factors that comprise beneficial and/or pathogenic microbes, as in chili (Capsicum spp.) and rice (Oryza sativa) plants [18,19], and may thus have biotechnological applications in agriculture. In clonal plants, vegetative reproduction may also transmit microbes, e.g.…”

Section: Assessing Plant Microbiota Diversity and Its Drivers: From Local To Global Inventoriesmentioning

confidence: 99%

The plant microbiota: composition, functions, and engineering

Chialva

Lanfranco

Bonfante

2022

Current Opinion in Biotechnology

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Plants growing in nature live in association with beneficial, commensal, and pathogenic microbes, which make up the plant microbiota. The close interaction between plants and their microbiotas has raised fundamental questions about plant responses to these microbes and the identity of the main factors driving microbiota structure, diversity, and function in bulk soil, in the rhizosphere, and in the plant organs. Beneficial microorganisms have long been used as inoculants for crops; the current development of synthetic microbial communities and the identification of plant traits that respond to the microbiota form the basis for rational engineering of the plant microbiota to improve sustainable agriculture.

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“…ACC deaminase enzyme production was qualitatively determined using Penrose and Glick's methodology [50]. IAA production was qualitatively determined using Dowarah's methodology [51]. NH 3 production was qualitatively determined using Dutta and Thakur's methodology [52].…”

Section: Assessment Of Pgp Potentialmentioning

confidence: 99%

In Vitro Study of Biocontrol Potential of Rhizospheric Pseudomonas aeruginosa against Pathogenic Fungi of Saffron (Crocus sativus L.)

Wang

Sun

et al. 2021

Pathogens

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Plant rhizosphere soil contains a large number of plant-growth promoting rhizobacteria, which can not only resist the invasion of pathogenic microorganisms and protect plants from damage, but also promote the growth and development of plants. In this study, Pseudomonas aeruginosa strain YY322, isolated and screened from the rhizosphere soil of saffron (Crocus sativus L.), was found through a plate confrontation experiment to show highly effectual and obvious antagonistic activity against the pathogens of saffron, including Fusarium oxysporum, Fusarium solani, Penicillium citreosulfuratum, Penicillium citrinum and Stromatinia gladioli. In addition, the volatile organic compounds of strain YY322 had great antagonistic activity against these pathogens. Observation under a scanning electron microscope and transmission electron microscope reflected that strain YY322 had a significant effect on the hyphae and conidia of F. oxysporum and F. solani. Through the detection of degrading enzymes, it was found that P. aeruginosa can secrete protease and glucanase. The plant growth promoting performance was evaluated, finding that strain YY322 had the functions of dissolving phosphorus, fixing nitrogen, producing siderophore and producing NH3. In addition, whole genome sequencing analysis indicated that the YY322 genome is comprised of a 6,382,345-bp circular chromosome, containing 5809 protein-coding genes and 151 RNA genes. The P. aeruginosa YY322 genome encodes genes related to phenazine (phzABDEFGIMRS), hydrogen cyanide(HCN) (hcnABC), surfactin (srfAA), salicylate (pchA), biofilm formation (flgBCDEFGHIJKL, motAB, efp, hfq), and colonization (minCDE, yjbB, lysC). These results collectively indicated the role of P. aeruginosa YY322 in plant growth enhancement and biocontrol mechanisms. All in all, this study provides a theoretical basis for P. aeruginosa as the PGPR of saffron, paving the way for the subsequent development and utilization of microbial fertilizer.

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Evaluation of seed associated endophytic bacteria from tolerant chilli cv. Firingi Jolokia for their biocontrol potential against bacterial wilt disease

Cited by 29 publications

References 63 publications

The Phlorizin-Degrading Bacillus licheniformis XNRB-3 Mediates Soil Microorganisms to Alleviate Apple Replant Disease

The Phlorizin-Degrading Bacillus licheniformis XNRB-3 Mediates Soil Microorganisms to Alleviate Apple Replant Disease

The plant microbiota: composition, functions, and engineering

In Vitro Study of Biocontrol Potential of Rhizospheric Pseudomonas aeruginosa against Pathogenic Fungi of Saffron (Crocus sativus L.)

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