Evaluation of secondary metabolites from mangrove associated fungi <i>Meyerozyma guilliermondii</i>

Joel, Elsa Lycias; Bhimba, B. Valentin

doi:10.1016/j.ajme.2013.04.003

Cited by 22 publications

(9 citation statements)

References 16 publications

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“…Surprisingly, in our culture conditions, no antimicrobial activity was ever detected from the 24 assayed marine yeast isolates, identified as strains of Bullera alba , Meyerozyma guillermondii and Rhodotorula mucilaginosa species [ 15 ]. Some terrestrial representatives of those species were already demonstrated as antimicrobial peptide producers [ 17 , 18 , 19 , 20 ]. Alternatively, a high proportion of filamentous fungi exhibiting antimicrobial activities was revealed.…”

Section: Resultsmentioning

confidence: 99%

Deep Subseafloor Fungi as an Untapped Reservoir of Amphipathic Antimicrobial Compounds

et al. 2016

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The evolving global threat of antimicrobial resistance requires a deep renewal of the antibiotic arsenal including the isolation and characterization of new drugs. Underexplored marine ecosystems may represent an untapped reservoir of novel bioactive molecules. Deep-sea fungi isolated from a record-depth sediment core of almost 2000 m below the seafloor were investigated for antimicrobial activities. This antimicrobial screening, using 16 microbial targets, revealed 33% of filamentous fungi synthesizing bioactive compounds with activities against pathogenic bacteria and fungi. Interestingly, occurrence of antimicrobial producing isolates was well correlated with the complexity of the habitat (in term of microbial richness), as higher antimicrobial activities were obtained at specific layers of the sediment core. It clearly highlights complex deep-sea habitats as chemical battlefields where synthesis of numerous bioactive compounds appears critical for microbial competition. The six most promising deep subseafloor fungal isolates were selected for the production and extraction of bioactive compounds. Depending on the fungal isolates, antimicrobial compounds were only biosynthesized in semi-liquid or solid-state conditions as no antimicrobial activities were ever detected using liquid fermentation. An exception was made for one fungal isolate, and the extraction procedure designed to extract amphipathic compounds was successful and highlighted the amphiphilic profile of the bioactive metabolites.

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Section: Resultsmentioning

confidence: 99%

Deep Subseafloor Fungi as an Untapped Reservoir of Amphipathic Antimicrobial Compounds

et al. 2016

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“…stephensi , respectively. Ealier, Elsa Lycias Joel and Valentin Bhimba (2013) through GCMS analysis of marine fungus Meyerozyma guilliermondii found mainly secondary metabolites viz : Isohexyl neopentyl ester, Tridecane, Pentadecane, Isobutyl undecyl ester, 1-Nonadecanol, and 9-Octadecenoic acid. Lorenzen et al (1996) reported the isolation of 11-hydroxy-4-methyl-2,4,6-dodecatrienoic acid from Mucor sp.…”

Section: Discussionmentioning

confidence: 99%

Larvicidal, Histopathological, Antibacterial Activity of Indigenous Fungus Penicillium sp. Against Aedes aegypti L and Culex quinquefasciatus (Say) (Diptera: Culicidae) and Its Acetylcholinesterase Inhibition and Toxicity Assessment of Zebrafish (Danio rerio)

et al. 2019

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Fungal metabolites are considered to be most efficient tools to overcome the issues related to insecticide resistance and environmental pollution. The present study focus on the evaluation of the mosquito larvicidal efficacy of metabolites of seven indigenous fungal isolates (Penicillium sp. Aspergillus niger, A. flavus, A. parasiticus, Rhizopus sp. Mucor sp. and Aspergillus sp.) on the larvae of Aedes aegypti and Culex quinquefasciatus under the laboratory condition. The preliminary screening of the isolate, Penicillium sp. showed better larvicidal effect when compared to other fungi. The fungus was grown on Potato Dextrose Broth (PDB) in the laboratory (at 25°C) and maintained in the relative humidity (at 76 ± 4% for 15 days). Larvicidal potency of mycelial ethyl acetate extract (MEAE) of Penicillium sp. was performed against 1st to 4th instars larvae of Ae. aegypti and Cx. quinquefasciatus using four different concentrations (100, 200, 300, and 500 μg/ml) that showed better larval mortality values (μg/ml) of LC50 = 6.554, 5.487, 6.874, 6.892, and the LC90 = 11.486, 10.366, 12.879, 13.865 for Ae. aegypti and LC50 = 7.000, 13.943, 18.129, 25.212 and the LC90 = 12.541, 23.761, 30.923, 41.696 for Cx. quinquefasciatus. Exposure of metabolite to larvae resulted in behavior changes i.e., excitation, up and down with aggressive movement, anal papillae biting behavior. Further, the larvae treated with Penicillium sp. metabolite exhibited significant reduction in the levels of acetylcholinesterase. The 4th instar mosquito larvae treated with the 500 μg/ml mycelia extract showed severe histological damages. During the antibacterial analysis of Penicillium sp.- mycelium the maximum growth inhibition zone was recorded in Shigella dysenteriae (31.2 mm) and Klebsiella pneumoniae (31.1 mm) followed by others. In addition, to check the toxicity of Penicillium sp. MEAE against embryos of Zebrafish, a model system, using different concentrations of metabolites (1.0, 0.5, 0.125 mg/ml, 30, 3.0, and 0.5 μg/ml) and life-stage parameters were observed at 124 hpf. Furthermore, the Fourier Transformed Infrared and GCMS spectrum analysis of mycelium reflected several chemical compounds. The outcome of the study clearly shows that Penicillium sp. metabolites could serve as an ideal eco-friendly, single-step and inexpensive source for the control of Ae. aegypti and Cx. quinquefasciatus larvae.

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“…Other fungal species that have been isolated from Rhizophora are Phomopsis spp. from R. apiculata [ 6 ], Meyerozyma guilliermondii from the leaves of R. mucronata [ 48 ], Fusarium oxysporum from R. annamalayana [ 49 ], and two species of Pestalotiopsis from the twigs of two mangrove plants, R. apiculata and R. mucronata [ 7 ]; these fungal species have also produced secondary metabolites with various activities. Other Aspergillus isolated from various mangrove species include A. flavipes , from the inner leaves of Acanthus ilicifolius [ 50 ], Aspergillus sp., from the leaves of Ipomoea pes-caprae [ 51 ], A. flavus , from the roots of Hibiscus tiliaceus [ 52 ], and nine Aspergillus species, namely A. niger , A. fumigatus , A. aureoles , A. candidus , A. chevalier , A. flavus , A. glaucus , A. ochraceus , and A. smithii , from decomposing leaves of Ceriops tagal [ 53 ].…”

Section: Resultsmentioning

confidence: 99%

Insecticidal Activity of Ethyl Acetate Extracts from Culture Filtrates of Mangrove Fungal Endophytes

et al. 2015

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In the search for novel potent fungi-derived bioactive compounds for bioinsecticide applications, crude ethyl acetate culture filtrate extracts from 110 mangrove fungal endophytes were screened for their toxicity. Toxicity tests of all extracts against brine shrimp (Artemia salina) larvae were performed. The extracts with the highest toxicity were further examined for insecticidal activity against Spodoptera litura larvae and acetylcholinesterase (AChE) inhibition activity. The results showed that the extracts of five isolates exhibited the highest toxicity to brine shrimp at 50% lethal concentration (LC50) values of 7.45 to 10.24 ppm. These five fungal isolates that obtained from Rhizophora mucronata were identified based on sequence data analysis of the internal transcribed spacer region of rDNA as Aspergillus oryzae (strain BPPTCC 6036), Emericella nidulans (strains BPPTCC 6035 and BPPTCC 6038), A. tamarii (strain BPPTCC 6037), and A. versicolor (strain BPPTCC 6039). The mean percentage of S. litura larval mortality following topical application of the five extracts ranged from 16.7% to 43.3%. In the AChE inhibition assay, the inhibition rates of the five extracts ranged from 40.7% to 48.9%, while eserine (positive control) had an inhibition rate of 96.8%, at a concentration of 100 ppm. The extracts used were crude extracts, so their potential as sources of AChE inhibition compounds makes them likely candidates as neurotoxins. The high-performance liquid chromatography profiles of the five extracts differed, indicating variations in their chemical constituents. This study highlights the potential of culture filtrate ethyl acetate extracts of mangrove fungal endophytes as a source of new potential bioactive compounds for bioinsecticide applications.

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Evaluation of secondary metabolites from mangrove associated fungi Meyerozyma guilliermondii

Cited by 22 publications

References 16 publications

Deep Subseafloor Fungi as an Untapped Reservoir of Amphipathic Antimicrobial Compounds

Deep Subseafloor Fungi as an Untapped Reservoir of Amphipathic Antimicrobial Compounds

Larvicidal, Histopathological, Antibacterial Activity of Indigenous Fungus Penicillium sp. Against Aedes aegypti L and Culex quinquefasciatus (Say) (Diptera: Culicidae) and Its Acetylcholinesterase Inhibition and Toxicity Assessment of Zebrafish (Danio rerio)

Insecticidal Activity of Ethyl Acetate Extracts from Culture Filtrates of Mangrove Fungal Endophytes

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