Evaluation of (SD) MPT64 antigen rapid test, for fast and accurate identification of Mycobacterium tuberculosis complex

Hopprich, Ryan; Shephard, Lisa; Taing, Bopha; Kralj, Sonia; Smith, Annette; Lumb, Richard

doi:10.1097/pat.0b013e328359d565

Cited by 11 publications

(5 citation statements)

References 4 publications

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“…Of 27 studies, 12 studies used biochemistry method, 13,15,17-e19,25,27e29,31e33 8 used molecular method5,7e9,14,16,22,24 and 7 used biochemistry method plus molecular method as reference test. 20,21,23,26,30,34 Subgroup analysis by reference test showed that pooled sensitivity and specificity for biochemistry method, molecular method and biochemistry method plus molecular method were 94% (CI 93e95%) and 97% (CI 96e99%), 98% (CI 98e99%) and 99% (CI 98e99%), 99% (CI 98e99%) and 98% (CI 96e99%) respectively. There was also considerable heterogeneity (I 2 > 50%) for all the statistical measures.…”

Section: Subgroup Analysismentioning

confidence: 98%

Commercial MPT64-based tests for rapid identification of Mycobacterium tuberculosis complex: A meta-analysis

Yin¹,

Zheng

et al. 2013

Journal of Infection

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Section: Subgroup Analysismentioning

confidence: 98%

Commercial MPT64-based tests for rapid identification of Mycobacterium tuberculosis complex: A meta-analysis

Yin¹,

Zheng

et al. 2013

Journal of Infection

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“…Detection of MPT64 and antigen 85B via immunostaining of tissues and fine-needle aspirates can be rapid and sensitive methods for establishing an early and specific diagnosis of TB infection but are limited to pathology and microbiology laboratories ( 42 , 43 ). Detection of MPT64 in sputum cultures utilizing an immune-chromatographic assay, in conjunction with cording in a stained smear, has been used to make a preliminary yet high-confidence identification of M. tuberculosis complex in liquid culture ( 44 , 45 ). Thus, localization of infection and the low bacterial burden in TB disease usually restricts the specimen type for direct detection methods to sputum or invasively acquired tissues.…”

Section: Discussionmentioning

confidence: 99%

Potential of High-Affinity, Slow Off-Rate Modified Aptamer Reagents for Mycobacterium tuberculosis Proteins as Tools for Infection Models and Diagnostic Applications

et al. 2017

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Direct pathogen detection in blood to diagnose active tuberculosis (TB) has been difficult due to low levels of circulating antigens or due to the lack of specific, high-affinity binding reagents and reliable assays with adequate sensitivity. We sought to determine whether slow off-rate modified aptamer (SOMAmer) reagents with subnanomolar affinity for Mycobacterium tuberculosis proteins (antigens 85A, 85B, 85C, GroES, GroEL2, DnaK, CFP10, KAD, CFP2, RplL, and Tpx) could be useful to diagnose tuberculosis. When incorporated into the multiplexed, array-based proteomic SOMAscan assay, limits of detection reached the subpicomolar range in 40% serum. Binding to native M. tuberculosis proteins was confirmed by using M. tuberculosis culture filtrate proteins and fractions from infected macrophages and via affinity capture assays and subsequent mass spectrometry. Comparison of serum from culture-positive pulmonary TB patients and TB suspects systematically ruled out for TB revealed small but statistically significant (P < 0.0001) differences in the median M. tuberculosis signals and in specific pathogen markers, such as antigen 85B. Samples where many M. tuberculosis aptamers produced high signals were rare exceptions. In concentrated, protein-normalized urine from TB patients and non-TB controls, the CFP10 (EsxB) SOMAmer yielded the most significant differential signals (P < 0.0276), particularly in TB patients with HIV coinfection. In conclusion, direct M. tuberculosis antigen detection proved difficult even with a sensitive method such as SOMAscan, likely due to their very low, subpicomolar abundance. The observed differences between cases and controls had limited diagnostic utility in serum and urine, but further evaluation of M. tuberculosis SOMAmers using other platforms and sample types is warranted.

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“…Cultures were considered positive only after detection of MTBC by the immunochromatographic assay, SD BIOLINE TB Ag MPT64 (Standard Diagnostics, Seoul, South Korea). This assay is rapid, simple, and reliable with a demonstrated high sensitivity and specific on clinical isolates [ 10 , 11 ].…”

Section: Methodsmentioning

confidence: 99%

Multidrug-resistant tuberculosis in côte d'ivoire from 1995 to 2016: Results of national surveys

N’guessan

Ouassa²,

Dean

et al. 2018

EuJMI

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SettingTuberculosis (TB) drug resistance survey was conducted in 2016–2017 to estimate the burden of drug-resistant TB in Côte d'Ivoire.DesignA cross-sectional cluster-based survey was conducted. All eligible smear positive patients were interviewed using a structured questionnaire to collect clinical and sociodemographic information and tested by the Xpert Mycobacterium tuberculosis/rifampicin (MTB/RIF) assay. If resistant to rifampicin, solid and liquid cultures were performed. Phenotypic drug susceptibility testing (DST) was conducted in liquid medium for rifampicin, isoniazid, ethambutol, streptomycin, ofloxacin, and amikacin.ResultsOf the 1105 sputum smear positive patients enrolled, 995 new and 100 previously treated patients were positive for Mycobacterium tuberculosis complex by Xpert. Proportion of patients with rifampicin resistance was 4.6% (95% CI: 2.4–6.7) and 22% (95% CI: 13.7–30.3), respectively, for new and previously treated patients. Second-line DST results were available for most rifampicin-resistant patients. None were resistant to amikacin, only two were ofloxacin-resistant. Apart from the antecedent of previously treatment for TB, no other risk factors for rifampicin resistance were detected.ConclusionPrevalence of rifampicin resistance among TB patients in Côte d'Ivoire is higher than that in other countries in the region. Surveillance of drug resistance, through an expanded GeneXpert network, and programmatic management of drug-resistant TB (PMDT) must be strengthened in Côte d'Ivoire.

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Evaluation of (SD) MPT64 antigen rapid test, for fast and accurate identification of Mycobacterium tuberculosis complex

Cited by 11 publications

References 4 publications

Commercial MPT64-based tests for rapid identification of Mycobacterium tuberculosis complex: A meta-analysis

Commercial MPT64-based tests for rapid identification of Mycobacterium tuberculosis complex: A meta-analysis

Potential of High-Affinity, Slow Off-Rate Modified Aptamer Reagents for Mycobacterium tuberculosis Proteins as Tools for Infection Models and Diagnostic Applications

Multidrug-resistant tuberculosis in côte d'ivoire from 1995 to 2016: Results of national surveys

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