Evaluation of <scp>SmartStax</scp> and <scp>SmartStax PRO</scp> maize against western corn rootworm and northern corn rootworm: efficacy and resistance management

Head, Graham P.; Carroll, Matthew; Evans, Sean P.; Rule, D. M.; Willse, Alan; Clark, Thomas L.; Storer, Nicholas P.; Flannagan, Ronald D.; Samuel, Luke W.; Meinke, Lance J.

doi:10.1002/ps.4554

Cited by 190 publications

(196 citation statements)

References 33 publications

(72 reference statements)

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“…The Canadian Food Inspection Agency (CFIA) approved in September 2016 the commercialization and release of a new maize event, MON87411, which has the commercial name SmartStax PRO, expressing three Crystal ( Cry ) genes and a dsRNA containing a 240‐bp fragment of the D. virgifera Sucrose non-fermenting 7 ( DvSnf7 ) gene. In June 2017, the United States Environmental Protection Agency (US‐EPA) also approved this event for commercial planting. The DvSnf7 gene codes for an essential protein in vacuolar sorting and, until now, no insecticide has been developed that interferes with this process.…”

Section: Rnai Applications In Pest Managementmentioning

confidence: 99%

“…The RNAi‐based trait in SmartStax PRO results in the formation of a dsRNA transcript containing a 240‐bp fragment of the CRW Snf7 gene. Upon consumption, the plant‐produced dsRNA in MON87411 is specifically recognized by the RNAi machinery of CRW and other closely related CRW species, resulting in down‐regulation of the targeted Snf7 gene, leading to mortality . However, in many cases, the use of transgenic crops is not always realistic.…”

Section: Dsrna Deliverymentioning

confidence: 99%

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RNA interference technology in crop protection against arthropod pests, pathogens and nematodes

Zotti

Santos

Cagliari

et al. 2018

Pest Management Science

309

243

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Scientists have made significant progress in understanding and unraveling several aspects of double-stranded RNA (dsRNA)-mediated gene silencing during the last two decades. Now that the RNA interference (RNAi) mechanism is well understood, it is time to consider how to apply the acquired knowledge to agriculture and crop protection. Some RNAi-based products are already available for farmers and more are expected to reach the market soon. Tailor-made dsRNA as an active ingredient for biopesticide formulations is considered a raw material that can be used for diverse purposes, from pest control and bee protection against viruses to pesticide resistance management. The RNAi mechanism works at the messenger RNA (mRNA) level, exploiting a sequence-dependent mode of action, which makes it unique in potency and selectivity compared with conventional agrochemicals. Furthermore, the use of RNAi in crop protection can be achieved by employing plant-incorporated protectants through plant transformation, but also by non-transformative strategies such as the use of formulations of sprayable RNAs as direct control agents, resistance factor repressors or developmental disruptors. In this review, RNAi is presented in an agricultural context (discussing products that have been launched on the market or will soon be available), and we go beyond the classical presentation of successful examples of RNAi in pest-insect control and comprehensively explore its potential for the control of plant pathogens, nematodes and mites, and to fight against diseases and parasites in beneficial insects. Moreover, we also discuss its use as a repressor for the management of pesticide-resistant weeds and insects. Finally, this review reports on the advances in non-transformative dsRNA delivery and the production costs of dsRNA, and discusses environmental considerations.

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Section: Rnai Applications In Pest Managementmentioning

confidence: 99%

Section: Dsrna Deliverymentioning

confidence: 99%

RNA interference technology in crop protection against arthropod pests, pathogens and nematodes

Zotti

Santos

Cagliari

et al. 2018

Pest Management Science

309

243

View full text Add to dashboard Cite

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“…Suppression of Snf7 increases D. v. virgifera larval mortality and thereby reduces root damage . The RNAi trait will be combined with the Bt protein Cry3Bb1 for improved target pest control and resistance management …”

Section: Introductionmentioning

confidence: 99%

Responses of two ladybird beetle species (Coleoptera: Coccinellidae) to dietary RNAi

Haller

Widmer

Siegfried

et al. 2019

Pest Management Science

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BACKGROUND One concern with the adoption of RNAi‐based genetically engineered (GE) crops is the potential harm to valued non‐target organisms. Species of Coccinellidae (Coleoptera) are important natural enemies and might be exposed to the insecticidal dsRNA produced by the plant. To assess their susceptibility to dietary RNAi, we fed Adalia bipunctata and Coccinella septempunctata with a dsRNA designed to target the vATPase A of the western corn rootworm, Diabrotica virgifera virgifera (Dvv dsRNA). Specific dsRNAs designed to target the vATPase A of the two ladybird beetle species served as positive controls. RESULTS Our results revealed that both species were sensitive to dietary RNAi when ingesting their own dsRNAs, with C. septempunctata being more sensitive than A. bipunctata. Dvv dsRNA also adversely affected the two ladybird beetles as indicated by a significantly (but marginally) prolonged developmental time for A. bipunctata and a significantly reduced survival rate for C. septempunctata. These results, however, were obtained at Dvv dsRNA concentrations that were orders of magnitude higher than expected to occur in the field. Gene expression analyses confirmed the bioactivity of the dsRNA treatments and the results from the feeding bioassays. These results are consistent with the bioinformatics analyses, which revealed a higher number of 21‐nucleotide‐long matches, a requirement for effective RNAi, of the Dvv dsRNA with the vATPase A of C. septempunctata (34 matches) than with that of A. bipunctata (six matches). CONCLUSION Feeding bioassays revealed that two ladybird species are responsive to dietary RNAi. The two species, however, differed in their sensitivity. © 2019 Society of Chemical Industry

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“…13 Indeed, transgenic corn expressing dsRNA is currently being used to control western corn rootworm (WCR) by targeting vacuolar ATPase (V-ATPase). 14 dsRNAs can also be applied as crop sprays, 15 which enables use of synthetics to increase efficiency. Use of dsRNA in sprays is a very attractive mode of delivery as it eliminates the need for transgenics, which are not feasible to generate for some crops.…”

Section: Introductionmentioning

confidence: 99%

Guanidinium-Functionalized Interpolyelectrolyte Complexes Enabling RNAi in Resistant Insect Pests

2018

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RNAi-based technologies are ideal for pest control as they can provide species specificity and spare nontarget organisms. However, in some pests biological barriers prevent use of RNAi, and therefore broad application. In this study we tested the ability of a synthetic cationic polymer, poly-[N-(3-guanidinopropyl)methacrylamide] (pGPMA), that mimics arginine-rich cell penetrating peptides to trigger RNAi in an insensitive animal—Spodoptera frugiperda. Polymer–dsRNA interpolyelectrolyte complexes (IPECs) were found to be efficiently taken up by cells, and to drive highly efficient gene knockdown. These IPECs could also trigger target gene knockdown and moderate larval mortality when fed to S. frugiperda larvae. This effect was sequence specific, which is consistent with the low toxicity we found to be associated with this polymer. A method for oral delivery of dsRNA is critical to development of RNAi-based insecticides. Thus, this technology has the potential to make RNAi-based pest control useful for targeting numerous species and facilitate use of RNAi in pest management practices.

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Evaluation of SmartStax and SmartStax PRO maize against western corn rootworm and northern corn rootworm: efficacy and resistance management

Cited by 190 publications

References 33 publications

RNA interference technology in crop protection against arthropod pests, pathogens and nematodes

RNA interference technology in crop protection against arthropod pests, pathogens and nematodes

Responses of two ladybird beetle species (Coleoptera: Coccinellidae) to dietary RNAi

Guanidinium-Functionalized Interpolyelectrolyte Complexes Enabling RNAi in Resistant Insect Pests

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