Evaluation of salivary thiobarbituric acid reactive substances, total protein, and pH in children with various degrees of early childhood caries: a case–control study

Aliakbarpour, Fatemeh; Mahjoub, S.; Masrour-Roudsari, Jila; Seyedmajidi, Seyedali; Ghasempour, Maryam

doi:10.1007/s40368-021-00672-9

Cited by 4 publications

(9 citation statements)

References 26 publications

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“…Biuret reaction, Bradford, and Lowry were the most used methods for the outcome quantification (76.2%). Ten studies (47.6%) showed higher total protein concentration in the oral cavity of caries-affected individuals (47.6%) ( 11 – 13 , 28 , 72 , 75 , 78 , 85 , 90 , 96 ), whereas an opposite trend was observed in two studies ( 17 , 52 ). In the remaining nine studies (43%), no differences were found in the salivary total protein concentration among individuals.…”

Section: Resultsmentioning

confidence: 94%

“…Dental caries experience was determined based on the International Caries Detection and Assessment System (ICDAS) (five studies; 5.5%) ( 22 , 31 , 34 , 41 , 42 ). Twelve studies ( 13 , 21 , 43 – 52 ) did not describe how caries experience was determined. With the exception of four studies ( 12 , 19 , 53 , 54 ) that used other indexes, the remaining studies used the DMF index to assess dental caries experience (69 studies; 76.7%) ( 11 , 14 – 18 , 20 , 23 – 28 , 30 , 33 , 36 , 55 – 107 ).…”

Section: Resultsmentioning

confidence: 99%

“…Twenty-one studies reported salivary total protein concentration as outcome ( 11 – 13 , 16 – 17 , 20 , 24 , 25 , 28 , 46 , 52 , 54 , 56 , 58 , 63 , 72 , 75 , 79 , 85 , 90 , 96 ) ( Supplementary Table S2 ). Biuret reaction, Bradford, and Lowry were the most used methods for the outcome quantification (76.2%).…”

Section: Resultsmentioning

confidence: 99%

“…The TAC of saliva was reported in 19 studies ( 12 , 13 , 24 , 41 , 48 , 55 , 57 , 59 , 65 , 66 , 72 , 78 , 81 , 85 , 87 , 89 , 92 , 93 , 96 ) ( Supplementary Table S3 ) with ferric-reducing antioxidant power (FRAP) and thiobarbituric acid reactive substances (TBARS) being the methods most used (26% of studies each). Fifteen studies (79%) ( 12 , 13 , 41 , 48 , 55 , 59 , 65 , 66 , 72 , 78 , 81 , 85 , 87 , 93 , 96 ) showed that caries-affected individuals present higher TAC. Higher TAC in caries-free individuals was found in one study ( 89 ) and no differences were found among individuals in the other three remaining studies ( 24 , 57 , 92 ).…”

Section: Resultsmentioning

confidence: 99%

“…Previous studies have indicated some association between salivary proteins or salivary enzymes and dental caries susceptibility. Caries-affected individuals may present higher total salivary protein concentration ( 11 – 13 ), higher levels of secretory immunoglobulin-A (s-IgA) ( 14 – 16 ), higher concentrations of mucin ( 17 ), higher carbonic anhydrase IV (CA-IV) activity ( 18 , 19 ), higher lysozyme activity ( 20 ), higher levels of human beta-defensin (hBD) ( 21 , 22 ), and lower levels of LL-37 ( 23 ), among other differences. However, contradictory results have also been reported ( 24 – 37 ).…”

Section: Introductionmentioning

confidence: 99%

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Comparison of oral cavity protein abundance among caries-free and caries-affected individuals—a systematic review and meta-analysis

Silveira,

Prato,

Pilati

et al. 2023

Front. Oral. Health

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ObjectiveSome salivary proteins seem to be differently abundant among caries-free (CF) and caries-affected (CA) individuals, but previous results are contradictory precluding that definitive conclusion be drawn. A pooled analysis of the available evidence may provide more robust data on identifying oral cavity protein patterns among CF and CA individuals. This systematic review and meta-analysis (PROSPERO CRD42021269079) aimed to compare the oral cavity protein abundance among caries-free and caries-affected individuals.MethodsThis study was conducted following PRISMA guidelines. PubMed, Embase, and Web of Science databases were systematically assessed (up to February 2023) to retrieve clinical studies written in English, German, or in Latin-based languages that compared the oral cavity protein abundance among CF and CA individuals. Data extraction and methodological quality assessment (NIH guidelines) were independently performed by two investigators. Qualitative synthesis was performed from all included studies and meta-analysis was performed using a random-effects model with inverse variance for studies that reported the concentration of proteins or enzymatic activity. Standardized mean difference (SMD) with respective 95% confidence interval (CI) were calculated for each outcome.ResultsA total of 90 studies (two cohort and 88 cross-sectional designs) of more than 6,000 participants were selected for data extraction, being the quality of evidence graded as “fair” for most of them. The oral cavity of CF individuals presented lower total protein concentration [SMD = 0.37 (95% CI: 0.07–0.68; 18 studies)], lower total antioxidant capacity [SMD = 1.29 (95% CI: 0.74–1.85); 17 studies], and lower carbonic anhydrase activity [SMD = 0.83 (95% CI: 0.58–1.09); three studies], whereas CA individuals presented lower carbonic anhydrase concentration [SMD = −0.66 (95% CI: −1.00 to −0.32); three studies], urease [SMD = −0.95 (IC 95%: −1.72 to −0.17); four studies], and arginine deiminase system [SMD = −2.07 (95% CI: −3.53 to −0.62); three studies] activities. Antimicrobial peptides, secretory immunoglobulin-A concentrations and alpha-amylase activity were similar among individuals.ConclusionDifferences on oral cavity protein abundance were observed among CF and CA individuals. These data indicate some protein patterns for the oral health and dental caries conditions. Even when statistically significant, some of the results were not very consistent. Cohort studies need to be conducted to validate these results.

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Section: Resultsmentioning

confidence: 94%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Comparison of oral cavity protein abundance among caries-free and caries-affected individuals—a systematic review and meta-analysis

Silveira,

Prato,

Pilati

et al. 2023

Front. Oral. Health

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Dental Caries and Salivary Oxidative Stress: Global Scientific Research Landscape

et al. 2023

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This study aimed to analyze the research trends on salivary oxidative stress associated with dental caries and to perform bibliometric approaches for existing publications on this association. A search was performed using the Web of Science Core Collection, without any restriction of language or publication year. The number of periodicals with the most published articles in this theme, most published authors and keywords were mapped; other metrics were also evaluated such as the countries that have more research on the subject and the period in which there were more publications on the subject. During the knowledge mapping, the most frequent experimental designs were analyzed, type of saliva collection, stage of caries disease, evaluated oxidative parameters were retrieved and analyzed from each manuscript. Between the 43 selected articles, the Journal of Clinical Pediatric Dentistry was the periodical appearing the most with 4 published articles. The authors who published the most were Celec, P., Tothova, L., Hegde, A.M., Shetty, S., Antoniali, C., and Pessan, JP with three articles each, and a total of 180 keywords representing the evolution of the theme. India and Asia were found to be the country and continent with most publications, respectively. Most articles collected non-stimulated total saliva, with total antioxidant capacity being the parameter most often evaluated. The type of study that appeared the most was cross-sectional studies, and articles published in the period of 2017–2022 were the most frequent. Studies show that dental caries can be associated to the changes in salivary oxidative biochemistry with an increase in lipid peroxidation, a biomarker of oxidative damage, and an increase in antioxidant capacity in chronic caries, in response to cariogenic challenge. Some studies evidence the reduction of lipid peroxidation after treatment of the carious lesion. Our findings reveal worldwide research trends, as well as a clearer knowledge of the evolution and future scenarios of this issue, also showing the mechanisms associating dental caries with changes in salivary oxidative biochemical parameters are not clear.

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Are Dental Caries Associated with Oxidative Stress in Saliva in Children and Adolescents? A Systematic Review

et al. 2022

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Background: This systematic review aimed to assess whether dental caries is associated with oxidative salivary stress. Methods: The searches were carried out in electronic databases, including PubMed, Scopus, Web of Science, the Cochrane Library, LILACS, OpenGrey, and Google Scholar, without restrictions on the date of publication and language. The acronym PECO was used, in which the participants (P) were children and adolescents exposed (E) to dental caries compared (C) to those without dental caries, with the outcome (O) of modulation of oxidative biochemical parameters. After the search retrieval, the duplicates were removed, and the articles were evaluated by title and abstract, following the inclusion and exclusion criteria. Then, the papers were read and thoroughly assessed. After selection, the risk of bias assessment and qualitative synthesis were performed using the Newcastle-Ottawa Scale (NOS) for observational studies. The Grading of Recommendations, Assessment, Development, and Evaluation (GRADE) tool was used to assess the level of evidence. Results: A total of 5790 studies were found, and 30 articles were considered eligible and were included for the qualitative synthesis and the level of evidence assessment. The studies showed an imbalance of the antioxidant and pro-oxidant parameters in individuals with dental caries, with primarily increases in both total antioxidant capacity and lipid peroxidation. Most articles showed a low risk of bias, having comparability as the main issue. When exploring through GRADE, a very low level of evidence was found. Conclusions: It was possible to observe an association between oxidative stress and dental caries, showing a disbalance of antioxidants and pro-oxidants, but the evidence level was still very low.

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Evaluation of salivary thiobarbituric acid reactive substances, total protein, and pH in children with various degrees of early childhood caries: a case–control study

Cited by 4 publications

References 26 publications

Comparison of oral cavity protein abundance among caries-free and caries-affected individuals—a systematic review and meta-analysis

Comparison of oral cavity protein abundance among caries-free and caries-affected individuals—a systematic review and meta-analysis

Dental Caries and Salivary Oxidative Stress: Global Scientific Research Landscape

Are Dental Caries Associated with Oxidative Stress in Saliva in Children and Adolescents? A Systematic Review

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