Evaluation of salivary interleukin-6 in children with early childhood caries after treatment

Abstract

Background:The role of cytokines as a marker in the oral inflammatory process in ECC has not been fully explored before and after full mouth rehabilitation.Aims:The aim of this study was to assess the level of salivary interleukin-6 (IL-6) in children with ECC and to compare its levels before and after comprehensive full mouth rehabilitation.Methods and Materials:Saliva samples were collected from children with ECC prior to dental treatment and 3-month post treatment. The salivary IL-6 levels were analyzed using the ELISA method. The gingival index was also timely recorded. Oral health awareness sessions were conducted for children and their parents at regular intervals during the 3-month study period. Statistical analysis used: Wilcoxon Signed Rank test compared the levels of salivary IL-6 while, the paired t test compared the values of gingival index before and after treatment.Results:The mean level of salivary IL-6 before and 3 months after treatment had reduced and this reduction was statistically significant (P < 0.000). The gingival index scores had also reduced significantly 3-months post treatment (P < 0.002).Conclusions:Children with ECC when completely rehabilitated and kept under frequent follow up, which includes reinforcement of oral hygiene measures and maintaining a low caries activity state, the level of inflammation (IL-6) can definitely be minimized and thereby improving the quality of life of affected children.

Salivary protein roles in oral health and as predictors of caries risk

“…Cytokines IL-6, IL-8, IL-1β and TNF-α were reported to be increased significantly in dental caries compared with controls [ 135 , 200 , 201 , 202 ]. Although the role of cytokines in the mechanism that leads to dental caries is still not completely understood, a relevant correlation between salivary IL-1β concentration and S .…”

“…mutans can stimulate and modulate the production of pro-inflammatory cytokines [ 203 , 204 ]. According to Mennon et al [ 202 ], the salivary level of inflammatory IL-1β of children with ECC can be minimized after treatment and reinforcement of oral hygiene measures.…”

Salivary Pro-Inflammatory Cytokines and Salivary Bacterial Challenge Effect on Dental Caries: A Clinico-Molecular Cross-Sectional Study

“…In contrast to these studies, Cogulu et al point out that dental caries was not significantly correlated with salivary or serum concentrations of IL-1β, IL-1ra, or IL-10 4 . Many studies suggest a strong correlation between IL-6 and dental caries (17,24,29). In our study, we observed that IL-6 and IL-8 levels increased in patients with high caries.…”

“…In addition, investigators believe that high levels of IL-6 in saliva may be associated with metabolic disorders, and that increased levels of cytokine in serum may be related to metabolic syndrome and hyperinsulinemia (17,29). Systematic reviews have shown that excessive sugar consumption increases insulin resistance and leads to metabolic disorders.…”

Efficacy of a calcium sucrose phosphate based toothpaste in elevating the level of calcium, phosphate ions in saliva and reducing plaque: A clinical trial

“…Passive drooling method with minimal oral movements as described in previous studies was used to collect the unstimulated saliva. [ 9 10 11 ] Following that, study participants from their respective groups were asked to brush using the paste allotted to them. Half an hour and 6 h after brushing, saliva samples were again collected (Sample B and Sample C, respectively) from both the groups to evaluate the substantivity of the toothpaste in the oral cavity.…”