Evaluation of root yield traits and glucosinolate concentration of different Armoracia rusticana accessions in Basilicata region (southern Italy)

Agneta, Rosa; Möllers, Christian; Maria, Susanna De; Rivelli, Anna Rita

doi:10.1016/j.scienta.2014.03.025

Cited by 14 publications

(11 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In our previous study (Agneta et al, 2014b) on six horseradish accession grown in a Mediterranean environment, we found total GLS concentrations ranging from 1.73 to 35.67 mmol/g DM, with SIN representing from 53% to 87% of the total GLS, NAS accounting from 5% to 15% and GBS from 4.7% to 8.6% of the total GLS. Also Wedelsbäck Bladh (2014), on a total of 168 Nordic accessions of horseradish found that SIN levels varied between 10 and 45 mmol/g DM, NAS between 1.3 and 7.4 mmol/g DM and GBS between 0.1 and 2.6 mmol/g DM, with accessions showing high levels of both SIN and NAS.…”

Section: Resultsmentioning

confidence: 73%

Evaluating strategies to improve glucosinolate concentration and root yield of field-grown horseradish in a Mediterranean environment: preliminary results

et al. 2016

Self Cite

View full text Add to dashboard Cite

Horseradish is grown for its enlarged taproot that is widely used as a dish condiment and as a source of horseradish peroxidase. Nowadays, the species is gaining great interest due to the richness in bioactive compounds that besides providing a high nutritional value are tested for innovative applications in different fields. Nevertheless, the effect of crop management on root yield and glucosinolates (GLS) biosynthesis is poorly documented. Aim of this study was to evaluate the root yield and GLS concentration of two field-grown horseradish accessions (Cor and Mon) grown with nitrogen (N) alone and both N and sulphur (S) (-N-S, +N-S and +N+S treatments) and harvested at different times [late autumn (LA), 2011 and 2012, early spring (ES), 2012]. Yield increased throughout the harvests up to 48% on average of the fertilised treatments and 25% in the unfertilised control. Conversely, root GLS concentration significantly declined in the unfertilised control throughout the harvests [from 7.6 in LA_2011 to 1.43 mmol/g dry matter (DM) in LA_2012] while it highly increased in plants grown with N alone and with both N and S by 46 and 98%, respectively, from LA_2011 to ES_2012 (up to 11.9 and 21.1 mmol/g DM, respectively); then it drastically decreased by 80% on average, in the next harvest. Among individual GLS, the concentration of sinigrin and nasturtin similarly varied as effect of the analysed factors, showing the highest values in Cor accession. The data show that although the level of GLS is highly dependent on genotype, fertilisation and harvesting date may play a primary role in determining the yield and GLS concentration in horseradish root.

show abstract

Section: Resultsmentioning

confidence: 73%

Evaluating strategies to improve glucosinolate concentration and root yield of field-grown horseradish in a Mediterranean environment: preliminary results

et al. 2016

Self Cite

View full text Add to dashboard Cite

show abstract

“…These differences seem to reflect the extremes found in other Brassicaceae species. For example Italian horseradish ( Armoracia rusticana ) roots can differ up to twenty times in GS concentration depending on the accession ( Agneta et al, 2014 ); American wild radish ( Raphanus raphanistrum ) accessions has more than 20x difference in GS concentrations in its secondary branches of plants from North Carolina vs. Mississippi ( Malik et al, 2010 ); and A. thaliana leaves can have extremes of more than 10x differences in total GS concentrations ( Kliebenstein et al, 2001b ). In Boechera stricta , there are also differences in both the total content (quantitative) and type (qualitative) of GS between genotypes ( Schranz et al, 2009 ; Manzaneda et al, 2010 ).…”

Section: Discussionmentioning

confidence: 99%

Flowering Locus C (FLC) Is a Potential Major Regulator of Glucosinolate Content across Developmental Stages of Aethionema arabicum (Brassicaceae)

et al. 2017

View full text Add to dashboard Cite

The biochemical defense of plants can change during their life-cycle and impact herbivore feeding and plant fitness. The annual species Aethionema arabicum is part of the sister clade to all other Brassicaceae. Hence, it holds a phylogenetically important position for studying crucifer trait evolution. Glucosinolates (GS) are essentially Brassicales-specific metabolites involved in plant defense. Using two Ae. arabicum accessions (TUR and CYP) we identify substantial differences in glucosinolate profiles and quantities between lines, tissues and developmental stages. We find tissue specific side-chain modifications in aliphatic GS: methylthioalkyl in leaves, methylsulfinylalkyl in fruits, and methylsulfonylalkyl in seeds. We also find large differences in absolute glucosinolate content between the two accessions (up to 10-fold in fruits) that suggest a regulatory factor is involved that is not part of the quintessential glucosinolate biosynthetic pathway. Consistent with this hypothesis, we identified a single major multi-trait quantitative trait locus controlling total GS concentration across tissues in a recombinant inbred line population derived from TUR and CYP. With fine-mapping, we narrowed the interval to a 58 kb region containing 15 genes, but lacking any known GS biosynthetic genes. The interval contains homologs of both the sulfate transporter SULTR2;1 and FLOWERING LOCUS C. Both loci have diverse functions controlling plant physiological and developmental processes and thus are potential candidates regulating glucosinolate variation across the life-cycle of Aethionema. Future work will investigate changes in gene expression of the candidates genes, the effects of GS variation on insect herbivores and the trade-offs between defense and reproduction.

show abstract

“…As most Brassicaceae plants have no mycorrhizae (Pongrac et al, 2013), they are good candidates to study the assembly of the endophytic community. The very high amount of GSLs in horseradish (Armoracia rusticana) (Agneta et al, 2014;Gonda et al, 2016) makes it an excellent model to study correlations between abundances of chemical and endophytic features.…”

Section: Introductionmentioning

confidence: 99%

Correlations Between the Metabolome and the Endophytic Fungal Metagenome Suggests Importance of Various Metabolite Classes in Community Assembly in Horseradish (Armoracia rusticana, Brassicaceae) Roots

et al. 2022

View full text Add to dashboard Cite

The plant microbiome is an increasingly intensive research area, with significance in agriculture, general plant health, and production of bioactive natural products. Correlations between the fungal endophytic communities and plant chemistry can provide insight into these interactions, and suggest key contributors on both the chemical and fungal side. In this study, roots of various horseradish (Armoracia rusticana) accessions grown under the same conditions were sampled in two consecutive years and chemically characterized using a quality controlled, untargeted metabolomics approach by LC-ESI-MS/MS. Sinigrin, gluconasturtiin, glucoiberin, and glucobrassicin were also quantified. Thereafter, a subset of roots from eight accessions (n = 64) with considerable chemical variability was assessed for their endophytic fungal community, using an ITS2 amplicon-based metagenomic approach using a custom primer with high coverage on fungi, but no amplification of host internal transcribed spacer (ITS). A set of 335 chemical features, including putatively identified flavonoids, phospholipids, peptides, amino acid derivatives, indolic phytoalexins, a glucosinolate, and a glucosinolate downstream product was detected. Major taxa in horseradish roots belonged to Cantharellales, Glomerellales, Hypocreales, Pleosporales, Saccharomycetales, and Sordariales. Most abundant genera included typical endophytes such as Plectosphaerella, Thanatephorus, Podospora, Monosporascus, Exophiala, and Setophoma. A surprising dominance of single taxa was observed for many samples. In summary, 35.23% of reads of the plant endophytic fungal microbiome correlated with changes in the plant metabolome. While the concentration of flavonoid kaempferol glycosides positively correlated with the abundance of many fungal strains, many compounds showed negative correlations with fungi including indolic phytoalexins, a putative glucosinolate but not major glucosinolates and a glutathione isothiocyanate adduct. The latter is likely an in vivo glucosinolate decomposition product important in fungal arrest. Our results show the potency of the untargeted metabolomics approach in deciphering plant–microbe interactions and depicts a complex array of various metabolite classes in shaping the endophytic fungal community.

show abstract

Evaluation of root yield traits and glucosinolate concentration of different Armoracia rusticana accessions in Basilicata region (southern Italy)

Cited by 14 publications

References 26 publications

Evaluating strategies to improve glucosinolate concentration and root yield of field-grown horseradish in a Mediterranean environment: preliminary results

Evaluating strategies to improve glucosinolate concentration and root yield of field-grown horseradish in a Mediterranean environment: preliminary results

Flowering Locus C (FLC) Is a Potential Major Regulator of Glucosinolate Content across Developmental Stages of Aethionema arabicum (Brassicaceae)

Correlations Between the Metabolome and the Endophytic Fungal Metagenome Suggests Importance of Various Metabolite Classes in Community Assembly in Horseradish (Armoracia rusticana, Brassicaceae) Roots

Contact Info

Product

Resources

About