Evaluation of Root and Leaf Extracts of Glycrriza Glabra for Antimicrobial Activity

Abstract

Glycyrrhiza glabra is a traditional herb which grows in various parts of the world. It is a very sweet, moist, soothing herb that detoxifies and protects the liver. It also has a powerful anti-inflammatory action and also finds applications in arthritis and mouth ulcers. In the present study, antibacterial activity of Glycyrrhiza glabra root and leaf extracts were tested against Escherichia coli, Pseudomonas aeruginosa, Entereobacter cloacae and Klebsiella sp. using agar well/disc diffusion assay. The extracts obtained by cold maceration method and Soxhlet method could not inhibit the test bacteria. Both the leaf and root extracts prepared using reflux extraction method exhibited strong antibacterial activity against Escherichia coli and Pseudomonas aeruginosa. However these were found to be ineffective against Enterobacter cloacae and Klebsiella sp. The MIC of Glycyrrhiza glabra roots were 3µg/ml and 1µg/ml for E. coli and P. aeruginosa, respectively. The phytochemical analysis revealed the presence of tannins in leaf and root extracts and the concentration was found to be 3.161 and 2.455mg/ml, respectively at 530nm. This could be the possible explanation for the inhibitory effect of the extracts against the test organisms.  He has guided more than 60 M.Sc, 4 M.Phil and 7 PhD students. He has published more than 54 research papers in various journals of national and international repute. He has completed three major research project sponsored by various state and national agency. His current research interest lying on the "Functional Genomics of Plant Secondary Metabolism".

Antioxidant, Cytotoxic, and Antimicrobial Activities of Glycyrrhiza glabra L., Paeonia lactiflora Pall., and Eriobotrya japonica (Thunb.) Lindl. Extracts

“…Few antimicrobial studies of the leaf extract of E. japonica have been conducted and it did not show toxicity against the six bacterial strains here. The G. glabra extract showed antimicrobial activity in some other bacterial strains [61,62,63,64,65] and medium effect against five bacterial strains in this study. The P. lactiflora extract was reported to exhibit antibacterial and antiviral activity [66,67]; its antibacterial effect was strong on some bacteria species here.…”

Therapeutic Response of Epimedium gandiflorum’s Different Doses to Restore the Antioxidant Potential and Reproductive Hormones in Male Albino Rats

“… 11 In addition medicinal plants used as traditional medicine are easily consumable, easily available and cheaper with simple preparation strategies to mediate beneficial effects. 12 , 13 Moreover, it was reported that 80% population worldwide and more than 30% of pharmaceutical formulations are dependent upon medicinal plants, reported by WHO. 14 The current study was planned with the main objectives to determine the phytochemical constituents of E. grandiflorum leaves aqueous hydroethanolic extract, therapeutic response by determining the antioxidant parameters and reproductive hormones as well as structure of testicular tissue in CCl 4 intoxicated male albino rats.…”

The effect of aquous extract of johar leaves (Senna siamea) injection on survival rate and blood profile of snakehead fish (Channa striata)

“…The leaves were then mashed with a blender and stored at room temperature in a closed place. Johar leaves extraction was carried out according to the method of Aggarwal et al (2015), by dissolving 60 g of johar leaves powder with 300 ml of sterile distilled water. The mixture of johar leaves extract with distilled water was kept at 65°C for 8 hours.…”