Evaluation of role of alpha-methyl acyl-coenzyme A racemase/P504S and high molecular weight cytokeratin in diagnosing prostatic lesions

Jain, Deepika; Gupta, Sumiti; Marwah, Nisha; Kalra, Rajnish; Gupta, Veena; Gill, Meenu; Jain, Nikita; Lal, Sunder; Sen, Rajeev

doi:10.4103/0973-1482.206239

Cited by 7 publications

(14 citation statements)

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“…The results of our study quite close to study done by Jain, et al and Djavan et al which states Gleason score 6 as the commonest pattern. [617] In our study, maximum number of cases, that is, 47.50% were moderately differentiated (Gleason score 7), followed by well differentiated, that is, 40% (Gleason score 3–6), and poorly differentiated that is, 12.5% (Gleason score 8–10). The results were in the similar line with the observations of Gleason who noted that majority of cases of malignant lesions of the prostate were of intermediate grade.…”

Section: Discussionmentioning

confidence: 52%

“…[8] Therefore, using AMACR as a positive marker along with a basal cell-specific negative marker, HMWCK (34βE12) and P63 will enhance diagnostic accuracy in prostate cancer and reduce the chance of misdiagnosis. [5678910]…”

Section: Discussionmentioning

confidence: 99%

“…[617] Patients with prostate cancer were in the higher age group as compared to patients with benign lesions.…”

Section: Discussionmentioning

confidence: 99%

“…The adjacent benign glands should not show more than weak, partial (no circumferential) staining if any. Negative staining pertained to no staining or focal, weak non-circumferential fine granular staining[6], whereas in case of HMWCK: Cytoplasmic positivity/negative and continuous/discontinuous. [8] Immunostaining for p63 was interpreted as positive/negative and continuous/discontinuous.…”

Section: Methodsmentioning

confidence: 99%

“…PSA is not a cancer-specific marker, as it is present in benign and malignant prostatic epithelial cells. [5] Basal cells are absent in prostate adenocarcinoma, high-molecular-weight cytokeratin (34βE12)[67] and P63[8910] immunostains specific for basal cells have been used for the diagnosis of prostate cancer. The identification of the basal cells of prostate glands indicates the presence of benign glands.…”

Section: Introductionmentioning

confidence: 99%

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Diagnostic utility of triple antibody (AMACR, HMWCK and P63) stain in prostate neoplasm

Rathod

Jaiswal

Bindu

2019

J Family Med Prim Care

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Section: Discussionmentioning

confidence: 52%

Section: Discussionmentioning

confidence: 99%

“…[617] Patients with prostate cancer were in the higher age group as compared to patients with benign lesions.…”

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Diagnostic utility of triple antibody (AMACR, HMWCK and P63) stain in prostate neoplasm

Rathod

Jaiswal

Bindu

2019

J Family Med Prim Care

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Evaluation of expression of immunohistochemical markers high molecular weight cytokeratin (HMWCK) and alpha-methylacyl coa racemose (AMACR) in prostatic needle biopsies and transurethral resection of prostate (TURP) specimen – A one year observational study

Kapoor,

Bannur

2024

JDPO

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In view of increasing incidence of Prostate cancer with age, its early detection and management is of utmost importance. Digital rectal examination, clinical picture and USG findings are non-specific. In prostatic lesions having a suspicious morphology, IHC staining (HMWCK and AMACR) is done to distinguish benign from malignant lesions. Absence of myoepithelial layer (HMWCK negative) along with cytoplasmic granular staining in glands (AMACR positive) is consistent with malignant diagnosis.To evaluate the utility of IHC markers HMWCK and AMACR in resolving morphologically suspicious foci on Prostatic needle core biopsies and TURP specimens.Observational StudyA total of 30 cases of prostatic lesions were studied. The specimens were fixed in 10% formalin and routinely processed. Haematoxylin-Eosin (H&E) and IHC staining (HMWCK and AMACR) was done in all 30 cases.Data collected was analyzed using appropriate statistical test.A total of 30 cases including 19 cases prostatic needle core biopsies and 11 cases of TURP specimens were included in our study. Histopathological diagnosis included 1 case each of Adenosis, Atypical adenomatous hyperplasia and Transitional cell metaplasia; 9 cases of BPH with suspicious foci, 4 cases of LGPIN, 3 cases of HGPIN and 11 cases Prostatic adenocarcinoma. In 5 cases including 3 cases of BPH with suspicious foci and 1 case each of adenosis and AAH, the diagnosis was changed to Prostatic Adenocarcinoma after IHC analysis. We conclude that IHC staining should be done in cases where routine H&E sections have an ambiguous morphology. HMWCK along with AMACR is a good marker combination to differentiate Benign from Malignant lesions.

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Diagnostic Value of Cytokeratin 34 beta E12 (Ck34βE12) and α-Methylacyl-CoA racemase (AMACR) Immunohistochemical Expression in Prostatic Lesions

2020

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Scan to discover online Background & Objective: Some prostatic lesions contain small suspicious foci for prostatic carcinoma in which the morphological features are equivocal. Two immunohistochemical markers namely, cytokeratin 34 beta E12 (Ck34βE12) and α-Methylacyl-CoA racemase (AMACR), were evaluated in these lesions for a definitive diagnosis and avoiding misdiagnosis or overdiagnosis of prostatic carcinoma. Methods: A total of 90 paraffin embedded blocks of prostatic tissue were selected and categorized into three groups as follows: 50 cases of benign prostatic hyperplasia (BPH), 20 cases of prostatic carcinoma, and 20 cases of benign prostatic lesions with suspicious foci labeled as ASAP (atypical small acinar proliferation) that occupy not more than 5% of the lesion. These cases were revised for histopathological diagnosis and stained with two immunohistochemical markers: Ck34βE12 and AMACR. Results: While 92.9% of BPH were positive for Ck34βE12, 96% of prostatic carcinoma were negative for this marker (P=0.0001). Regarding AMACR, 92.9% of BPH cases were negative, but 92% of prostatic carcinoma cases were positive for this marker (P=0.0001). Out of 20 cases of BPH, 15 cases containing suspicious foci showed Ck34βE12+/AMACR-(diagnosis: benign), but 5 cases were Ck34βE12-/AMACR+, for which the diagnosis changed to prostatic carcinoma (P=0.04). Conclusion: Immunohistochemical staining with Ck34βE12 and AMACR improved the diagnostic performance and increased confidence level for establishing definite diagnosis in cases with suspicious foci, in which the morphological features were equivocal. This could help to avoid misdiagnosis or overdiagnosis of prostatic carcinoma that would eventually improve the management of the patient and subsequently the prognosis.

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Evaluation of role of alpha-methyl acyl-coenzyme A racemase/P504S and high molecular weight cytokeratin in diagnosing prostatic lesions

Abstract: As an adjunct to biopsy, AMACR and HMWCK have value for resolving diagnostically challenging cases.

Cited by 7 publications

References 1 publication

Diagnostic utility of triple antibody (AMACR, HMWCK and P63) stain in prostate neoplasm

Diagnostic utility of triple antibody (AMACR, HMWCK and P63) stain in prostate neoplasm

Evaluation of expression of immunohistochemical markers high molecular weight cytokeratin (HMWCK) and alpha-methylacyl coa racemose (AMACR) in prostatic needle biopsies and transurethral resection of prostate (TURP) specimen – A one year observational study

Diagnostic Value of Cytokeratin 34 beta E12 (Ck34βE12) and α-Methylacyl-CoA racemase (AMACR) Immunohistochemical Expression in Prostatic Lesions

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