Evaluation of RIDA®GENE norovirus GI/GII real time RT-PCR using stool specimens collected from children and adults with acute gastroenteritis

Kanwar, Neena; Hassan, Ferdaus; Langley, Claire; Vinjé, Jan; Bryant, Patrick; George, Kirsten St.; Mosher, Laura B.; Matthews-Greer, Janice; Rocha, Maurilio Andrade; Beenhouwer, David O.; Harrison, Christopher J.; Moffatt, Mary; Shastri, Nirav; Selvarangan, Rangaraj

doi:10.1016/j.jcv.2018.04.006

Cited by 10 publications

(3 citation statements)

References 13 publications

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“…NoV genogroup GII was the most prevalent NoV (92.0%) in our study, similar to previous studies [ 7 , 8 , 26 ]. NoV GII is the most common genogroup detected in the United States and has been linked to more severe outcomes in outbreaks [ 27 ].…”

Section: Discussionsupporting

confidence: 93%

Multiplex PCR Pathogen Detection in Acute Gastroenteritis Among Hospitalized US Children Compared With Healthy Controls During 2011–2016 in the Post–Rotavirus Vaccine Era

Harrison

Hassan

Lee

et al. 2021

Open Forum Infectious Diseases

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Background Despite vaccine-induced decreases in US rotavirus (RV) disease, acute gastroenteritis (AGE) remains relatively common. We evaluated AGE pathogen distribution in hospitalized US children in the post-RV-vaccine era. Methods From December 2011 to June 2016, the New Vaccine Surveillance Network (NVSN) conducted prospective, active, population-based surveillance in hospitalized children with AGE. We tested stools from two NVSN sites (Kansas City; Houston) with Luminex x-TAG Gastrointestinal Pathogen Panels (Luminex GPP) and analyzed selected signs and symptoms. Results For 660 pediatric AGE inpatients and 624 age-matched healthy controls (HC), overall organism detection was 51.2% and 20.6% respectively, p <0.001. Among AGE subjects, GPP PCR detected >1 virus in 39% and >1 bacteria in 14% of specimens. Detection frequencies for AGE subjects vs. HC were: norovirus (NoV) 18.5% vs 6.6%, rotavirus (RV) 16.1% vs 9.8%, adenovirus 7.7% vs 1.4%, Shigella 4.8% vs 1.0%, Salmonella 3.1% vs 0.1%, Clostridioides difficile in ≥2-year olds 4.4% vs 2.4%. More co-detections occurred among AGE (37/660, 5.6%) than HC (14/624, 2.2%), p=0.0024. Per logistic regression analysis, ill contacts increased risk for NoV, RV and Shigella, p<0.001. More vomiting episodes occurred with NoV and RV, and more diarrheal episodes with Shigella and Salmonella. Modified Vesikari scores were highest for Shigella and lowest for C. difficile. Conclusion NoV detection was most frequent, however RV remained important in hospitalized AGE in the post-RV-vaccine era. Continued active surveillance is important to document ongoing vaccine effects, pathogen emergence and baseline disease burden for new vaccines.

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Section: Discussionsupporting

confidence: 93%

Multiplex PCR Pathogen Detection in Acute Gastroenteritis Among Hospitalized US Children Compared With Healthy Controls During 2011–2016 in the Post–Rotavirus Vaccine Era

Harrison

Hassan

Lee

et al. 2021

Open Forum Infectious Diseases

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“…In this study, a sample was considered positive if the Ct value obtained is less than 45. The RIDAGENE norovirus I and II has a high sensitivity and specificity for detection of norovirus in stool specimens from patients with diarrohea [ 24 ].…”

Section: Methodsmentioning

confidence: 99%

Molecular Characterization of Norovirus Strains Isolated from Older Children and Adults in Impoverished Communities of Vhembe District, South Africa

Mulondo

Khumela

Kabue

et al. 2020

Advances in Virology

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Background. Human norovirus (NoV) is an etiological agent associated with acute gastroenteritis (AGE) in both children and adults worldwide. However, very few studies have been reported on the prevalence and genetic diversity of NoV strains in children older than 5 years of age and adults with little or inadequate water and sanitation conditions. Objectives. The aim of this study was assessing the prevalence of the human norovirus in older children and adults suffering with diarrhoea from rural communities in the Vhembe district, Limpopo province. Methods. Between August 2017 and October 2018, stool samples were collected from outpatients suffering from AGE and screened for NoV strains using the RIDA©GENE norovirus I and II real-time one-step RT-PCR. RNA extracts of NoV-positive samples were subjected to RT-PCR amplification and nucleotide sequencing to genotype the positive NoV strains. Results. Out of 80 collected stool samples, 13 (16%) were tested positive for norovirus. Genogroup GII was identified in 6/13 (46%) samples and genogroup GI in 7/13 (54%) samples. The sequence analyses showed multiple genotypes including GII.Pg, GII.1, GII.2, GII.4, and GI.3. Phylogenetic analysis revealed the relatedness of NoV genotypes identified with other strains reported globally. Conclusion. Continued systematic surveillance to evaluate norovirus association with diarrhoea is needed to assist with epidemiological surveillance and disease burden in people of all the age groups.

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“…Single platform PCRs are simpler in design and implementation and avoid primer–primer competition. FDA-approved single-platform PCRs are the RIDA Gene norovirus GI/GII real-time RT-PCR (RGN-RT PCR) [ 75 ] and the Xpert Norovirus assay [ 73 , 76 , 77 ]. However, single platforms are less desirable in immunocompromised patients, as co-occurrence of other enteropathogens could be missed.…”

Section: Diagnostic Approachmentioning

confidence: 99%

Norovirus in Cancer Patients: A Review

Kondapi

Ramani

Estes

et al. 2021

Open Forum Infectious Diseases

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Norovirus (NoV) is the leading cause of viral-related diarrhea in cancer patients in whom it can be chronic, contributing to a decreased quality of life, interruption of cancer care, malnutrition, and altered mucosal barrier function. Immunosuppressed cancer patients shed NoV for longer periods of time than immunocompetent hosts favoring quasispecies development and emergence of novel NoV variants. While nucleic acid amplification tests (NAATs) for NoV diagnosis have revolutionized our understanding of NoV burden of disease; not all NAATs provide information on viral load, or infecting genotype. There is currently no effective antiviral or vaccine for chronic NoV infections. Screening for inhibitors of NoV replication in intestinal organoid culture models and creation of NoV specific adoptive T cells are promising new strategies to develop treatments for chronic NoV in immunosuppressed patients. Herein we summarize data on the epidemiology, clinical manifestations, diagnostic challenges and treatment of NoV infection in patients with cancer.

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Evaluation of RIDA®GENE norovirus GI/GII real time RT-PCR using stool specimens collected from children and adults with acute gastroenteritis

Cited by 10 publications

References 13 publications

Multiplex PCR Pathogen Detection in Acute Gastroenteritis Among Hospitalized US Children Compared With Healthy Controls During 2011–2016 in the Post–Rotavirus Vaccine Era

Multiplex PCR Pathogen Detection in Acute Gastroenteritis Among Hospitalized US Children Compared With Healthy Controls During 2011–2016 in the Post–Rotavirus Vaccine Era

Molecular Characterization of Norovirus Strains Isolated from Older Children and Adults in Impoverished Communities of Vhembe District, South Africa

Norovirus in Cancer Patients: A Review

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