Evaluation of rice as unregulated hidden allergen by fast real-time PCR

Pierboni, Elisa; Rondini, Cristina; Zampa, Sabrina; Tovo, Gloria Raquel; Altissimi, Serena; Haouet, Naceur

doi:10.1016/j.jcs.2020.102929

Cited by 1 publication

(1 citation statement)

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“…The significance threshold was set at P <0.05. Relative Repeatability Standard Deviation (RSDr) was calculated dividing the SDr by the mean of results and the data of PCR replicates were evaluated only where RSDr was ≤25% ( Pierboni et al, 2020 ).…”

Section: Methodsmentioning

confidence: 99%

Evaluation of the persistence of SARS-CoV-2 (ATCC® VR-1986HK™) on two different food contact materials: flow pack polyethylene and polystyrene food trays

Castrica

Balzaretti

Miraglia

et al. 2021

LWT

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Even though SARS-CoV-2’s primary transmission pathway is person-to-person, the role played by surfaces and food contact materials in carrying viral RNA should be further explored. For this purpose, the study aimed to investigate the persistence of SARS-CoV-2 using the strain ATCC® VR-1986HK™ on flow pack polyethylene (FPP) and polystyrene food trays (PFT). Samples of FPP and PFT were contaminated with heat-inactivated SARS-CoV-2 and were incubated at a temperature of 24 ± 1°C and at controlled relative humidity (RH 65%). The experimental design included analyses at the time 0, 3, 6, 12, 24, 36, 48 and after every 24 hours until the viral RNA was no longer detectable. The results showed a significant decrease ( P <0.001) in viral copies number on PFT within 3 hours (24% reduction) and, at 72 hours, the viral RNA had fallen below the limit of detection. Regarding the FPP, it was necessary to wait 24 hours for a significant decrease ( P =0.015) in the viral load (14% reduction), while the detection threshold was reached at 96 hours. These findings showed that the viral RNA persists longer on polyethylene flow pack samples than on polystyrene food trays, thus highlighting the importance of material characteristics in the persistence of SARS-CoV-2.

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Section: Methodsmentioning

confidence: 99%