Evaluation of resistance to Pseudomonas solanacearum in potato under controlled conditions

Abstract

Potato plantlets derived from in vitro propagation of three cultivars known for their field resistance (Cruza 148 and BR-63.65) or susceptibility (Drsirre) to Pseudomonas solanacearum E.E Smith were artificially inoculated under controlled conditions. The aim of this work was to determine the optimal inoculum concentration and the best observation period in which the cultivars would show different reactions to bacterial infection as expected on the basis of their field performance.A suitable statistical analysis of disease indices is proposed to distinguish between resistant and susceptible responses, with a particular care for the applicative needs and a univocal interpretation of the results. In order to evaluate the significance of sources of variation related to the observed mean differences, the analysis of variance and a convenient clustering procedure of disease index means were applied.The statistical analysis revealed that, under our conditions, an inoculum concentration of 5 x 106 cfu/plant was suitable for separating resistant from susceptible responses, in accordance with the reactions already observed in field experiments by other authors. Also, differences among the three cultivars were best observed nine to twelve days after inoculation with the pathogen.

Resistance to Ralstonia solanacearum of Sexual Hybrids Between Solanum commersonii and S. tuberosum

“…One-month-old vitro plants of each hybrid were transplanted into 60 mm diameter pots containing sterile soil in a temperature-controlled greenhouse. When a six to eight leaflet stage was reached, nine to 12 uniform plants for each genotype were inoculated pouring 1 mL of suspension into 1 cm deep holes previously made in the soil (Montanelli et al 1995). Three control plants were treated with SDW.…”