Evaluation of resazurin-based rapid test to detect colistin resistance in Acinetobacter baumannii isolates

Germ, Julija; Poirel, Laurent; Kišek, Tjaša Cerar; Špik, Vesna Cvitković; Seme, Katja; Premru, Manica Mueller; Zupanc, Tatjana Lejko; Nordmann, Patrice; Pirš, Mateja

doi:10.1007/s10096-019-03657-1

Cited by 16 publications

(17 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It showed a sensitivity and specificity of 41.2% and 86.1%, respectively [ 19 ]. Previous studies have evaluated the performance of the homemade test, the Rapid ResaPolymyxin NP test, using a collection of A. baumannii clinical isolates [ 16 , 17 ]. The sensitivity and specificity of the Rapid ResaPolymyxin NP test compared to the broth microdilution method was 100 and 96%, respectively, suggesting that this test is highly sensitive and specific for the detection of colistin resistance in A. baumannii [ 16 ].…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

RapidResa Polymyxin Acinetobacter NP® Test for Rapid Detection of Polymyxin Resistance in Acinetobacter baumannii

Bouvier

Sadek

Pomponio³

et al. 2021

Antibiotics

Self Cite

View full text Add to dashboard Cite

A homemade and culture-based test, relying on the visual detection of the reduction of the resazurin reagent (a cell viability indicator), has been developed for the rapid detection of polymyxin resistance in Acinetobacter baumannii. Here, we evaluated the industrial version of this test, the RapidResa Polymyxin Acinetobacter NP® test (Liofilchem, Italy). A well-characterized panel of 68 clinical A. baumannii strains (36 polymyxin-susceptible, 26 polymyxin-resistant A. baumannii, and 6 colistin-heteroresistant isolates) of worldwide origin was tested. All the colistin-susceptible A. baumannii isolates gave negative results according to the RapidResa Polymyxin Acinetobacter NP® test, except for a single isolate that gave a false-positive result. Out of the 26 colistin-resistant A. baumannii strains, 25 were correctly identified as colistin resistant using the RapidResa Polymyxin Acinetobacter NP® test. Only a single colistin-resistant A. baumannii strain gave a false-negative result. Additionally, the six colistin-heteroresistant isolates tested gave positive results. Altogether, the sensitivity and the specificity of the test were found to be 96% and 97%, respectively. The turn-around-time of this easy-to-perform test was 3-4h, which showed excellent reliability for identification of polymyxin resistance in A. baumannii. The RapidResa Polymyxin Acinetobacter NP® test allows a rapid differentiation between polymyxin-susceptible and -resistant A. baumannii isolates, which may contribute to optimization of the use of polymyxins for treating infections due to multidrug-resistant A. baumannii.

show abstract

Section: Resultsmentioning

confidence: 99%

“…Bacterial viability of A. baumannii isolates, after growth in medium with or without a defined concentration of colistin, is therefore evidenced [ 15 ]. The main value of this test is rapid categorization of susceptibility and resistance, which is important to optimally adapt the empirical treatment [ 16 , 17 ].…”

Section: Introductionmentioning

confidence: 99%

RapidResa Polymyxin Acinetobacter NP® Test for Rapid Detection of Polymyxin Resistance in Acinetobacter baumannii

Bouvier

Sadek

Pomponio³

et al. 2021

Antibiotics

Self Cite

View full text Add to dashboard Cite

show abstract

“…The different isolates had different effects on resazurin metabolism (S3 Fig). Resazurin has been proposed for high-throughput screening for antimicrobial assays due to it’s metabolism into the highly fluorescent resorufin [42–44], however, resorufin can be further metabolised into the non-fluorescent dihydroresourfin [42] leading to a transient fluorescent signal in some systems. Transient fluorescent signals can be seen in a number of the mastitis isolates (S3 Fig) and is not-related to generation time.…”

Section: Resultsmentioning

confidence: 99%

Exploiting open source 3D printer architecture for laboratory robotics to automate high-throughput time-lapse imaging for analytical microbiology

et al. 2019

View full text Add to dashboard Cite

Growth in open-source hardware designs combined with the low-cost of high performance optoelectronic and robotics components has supported a resurgence of in-house custom lab equipment development. We describe a low cost (below $700), open-source, fully customizable high-throughput imaging system for analytical microbiology applications. The system comprises a Raspberry Pi camera mounted on an aluminium extrusion frame with 3D-printed joints controlled by an Arduino microcontroller running open-source Repetier Host Firmware. The camera position is controlled by simple G-code scripts supplied from a Raspberry Pi singleboard computer and allow customized time-lapse imaging of microdevices over a large imaging area. Open-source OctoPrint software allows remote access and control. This simple yet effective design allows high-throughput microbiology testing in multiple formats including formats for bacterial motility, colony growth, microtitre plates and microfluidic devices termed ‘lab-on-a-comb’ to screen the effects of different culture media components and antibiotics on bacterial growth. The open-source robot design allows customization of the size of the imaging area; the current design has an imaging area of ~420 × 300mm, which allows 29 ‘lab-on-a-comb’ devices to be imaged which is equivalent 3480 individual 1μl samples. The system can also be modified for fluorescence detection using LED and emission filters embedded on the PiCam for more sensitive detection of bacterial growth using fluorescent dyes.

show abstract

“…Unfortunately, the CBDE and EDTA-CBDE methods are not recommended for use with Acinetobacter species due to high error rates, and large numbers of isolates are excluded from analysis due to the absence of a reference MIC (16). Recently, a resazurin-based rapid test to detect colistin resistance in Acinetobacter isolates was described and had a 93.3% sensitivity and specificity (26). This method does not distinguish between chromosome-or plasmid-mediated colistin resistance but provides an alternative to BMD to detect colistin resistance among Acinetobacter species.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the NG-Test MCR-1 Lateral Flow Assay and EDTA-Colistin Broth Disk Elution Methods To Detect Plasmid-Mediated Colistin Resistance among Gram-Negative Bacterial Isolates

et al. 2020

View full text Add to dashboard Cite

Plasmid-mediated colistin resistance (PMCR) is a global public health concern, given its ease of transmissibility. The purpose of this study was to evaluate two methods for the detection of PMCR from bacterial colonies: (i) the NG-Test MCR-1 lateral flow immunoassay (LFA; NG Biotech, Guipry, France) and (ii) the EDTA-colistin broth disk elution (EDTA-CBDE) screening test method. These methods were evaluated using a cohort of contemporary, clinical Gram-negative bacillus isolates from 3 U.S. academic medical centers (126 isolates of the Enterobacterales, 50 Pseudomonas aeruginosa isolates, and 50 Acinetobacter species isolates; 1 isolate was mcr positive) and 12 mcr-positive CDC-FDA Antibiotic Resistance (AR) Isolate Bank isolates for which reference broth microdilution colistin susceptibility results were available. Eleven (4.6%) isolates were strongly positive by the MCR-1 LFA, with an additional 8 (3.4%) isolates yielding faintly positive results. The positive percent agreement (PPA) and negative percent agreement (NPA) for MCR-1 detection were 100% and 96.1%, respectively. Upon repeat testing, only a single false-positive MCR-2 producer remained, as the isolates with initially faintly positive results were negative. The EDTA-CBDE screening method had an overall PPA and NPA of 100% and 94.3%, respectively. The NPA for the EDTA-CBDE method was slightly lower at 94.2% with Enterobacterales, whereas it was 96.0% with P. aeruginosa. The MCR-1 LFA and EDTA-CBDE methods are both accurate and user-friendly methods for the detection of PMCR. Despite the rarity of PMCR among clinical isolates in the United States, these methods are valuable tools that may be implemented in public health and clinical microbiology laboratories to further discern the mechanism of resistance among colistin-resistant Gram-negative isolates and to detect PMCR for infection prevention and control purposes.

show abstract

Evaluation of resazurin-based rapid test to detect colistin resistance in Acinetobacter baumannii isolates

Cited by 16 publications

References 9 publications

RapidResa Polymyxin Acinetobacter NP® Test for Rapid Detection of Polymyxin Resistance in Acinetobacter baumannii

RapidResa Polymyxin Acinetobacter NP® Test for Rapid Detection of Polymyxin Resistance in Acinetobacter baumannii

Exploiting open source 3D printer architecture for laboratory robotics to automate high-throughput time-lapse imaging for analytical microbiology

Evaluation of the NG-Test MCR-1 Lateral Flow Assay and EDTA-Colistin Broth Disk Elution Methods To Detect Plasmid-Mediated Colistin Resistance among Gram-Negative Bacterial Isolates

Contact Info

Product

Resources

About