Evaluation of renal protective effects of the green-tea (EGCG) and red grape resveratrol: role of oxidative stress and inflammatory cytokines

Abstract

Epigallocatechin-gallate (EGCG) and resveratrol (RSVL) are two of the most promising natural medicines. We verified their capacity to ameliorate cisplatin (CP)-induced disruption of renal glomerular filtration rate (GFR) in rats, and sought the mediatory involvement of lipid peroxidation (malondialdehyde [MDA]-level) and inflammatory cytokine (TNF-α) therein. CP (10 mg kg⁻¹), a single i.p. dose, disrupted GFR (11-fold-rise in proteinuria, 2-5-fold rise in serum creatinine/urea levels) after 7 days, and killed all animals after 10 days. Kidney-homogenates from CP-treated rats displayed higher MDA and TNF-α, but lower reduced-glutathione (GSH) levels. Rats treated with EGCG (50 mg kg⁻¹, but not 25 mg kg⁻¹) had no fatalities and showed significantly-recovered GFR; while their kidney-homogenates had markedly reduced MDA, TNF-α and enhanced GSH levels at 7 days. Conversely, RSVL or quercetin (25, 50 mg kg⁻¹) neither improved GFR nor reduced (MDA)/TNF-α levels after 7 days. Resuming treatment with 50 mg kg⁻¹ for 10 days rescued only 25% of animals (p > 0.05). Correlation studies showed a significant association between creatinine level, and each of MDA (r = 0.91), GSH (r = -0.87), and TNF-α (0.91). The study showed for the first time that EGCG, unlike RSVL, can protect against CP-induced nephrotoxicity. At the molecular level, CP triggers a high level of oxidative stress and systemic inflammation, events that were all abrogated with EGCG; better than RSVL or quercetin.

Epigallocatechin-3-gallate protects against cisplatin-induced nephrotoxicity by inhibiting endoplasmic reticulum stress-induced apoptosis

“…32 It has been also shown that EGCG can attenuate cigarette smoke-induced cardiomyocyte apoptosis through the inhibition of mitochondrial dependent apoptotic pathway. 17 Renal tubular apoptosis plays an important role in CP-induced nephrotoxicity.…”

EGCG Enhances the Efficacy of Cisplatin by Downregulating hsa-miR-98-5p in NSCLC A549 Cells

“…In 8 ovarian cancer cell lines, EGCG treatment led to an enhanced intracellular H 2 O 2 generation, suggesting that EGCG might accentuate oxidative stress to sensitize ovarian cancer cells to cisplatin (15). On the other hand, EGCG could protect the tissues and organs from nephrotoxicity, neurotoxicity, and ototoxicity of cisplatin (16)(17)(18).…”

Protective effects of green tea polyphenol against cisplatin-induced nephrotoxicity in rats

“…EGCG has been reported to have a preventive and treatment effects on several types of chronic diseases along with its anticancer effects [13,14]. As a study that reported about the inhibitory effect of EGCG on the toxicity of CP, El-Mowafy et al [15] reported about the preventive effect of renal damage by performing Intraperitoneal injection of EGCG for the prevention of renal damages that were caused by CP. Sahin et al [16] reported about that the injection of EGCG can reduce the oxidative renal damages and inflammative reactions that were caused by CP.…”