Evaluation of real‐time polymerase chain reaction for the diagnosis of protozoal myeloencephalitis in horses using cerebrospinal fluid
Carla Katherine Enriquez,
Jennifer K. Morrow,
Amy Graves
et al.
Abstract:BackgroundEquine protozoal myeloencephalitis (EPM) caused by Sarcocystis neurona remains an antemortem diagnostic challenge in some horses. Recent work suggested the use of real‐time PCR (rtPCR) on cerebrospinal fluid (CSF) as a promising diagnostic tool.ObjectiveTo evaluate the sensitivity and specificity of S. neurona rtPCR on CSF for EPM diagnosis using horses with EPM and S. neurona‐seropositive horses with other neurologic conditions.AnimalsNinety‐nine horses with neurologic disease that underwent complet… Show more
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