Evaluation of Reactive Oxygen Species Scavenging of Polydopamine with Different Nanostructures

Abstract

Reactive oxygen species (ROS) play an important role in cellular metabolism and many oxidative stress‐related diseases, while excessive accumulation of ROS will lead to genetic changes in cells and promote the occurrence of inflammatory diseases or cell death. Nature‐inspired polydopamine (PDA) with tailored nanostructures emerges as an ROS scavenger and is considered as an effective approach to inflammation‐related diseases. However, the effects of nanoparticle structure on PDA scavenging efficacy and efficiency remain uncovered. In this work, three typical PDA nanoparticles including solid PDA, mesoporous PDA, and hollow PDA are synthesized, and of which physiochemical properties are characterized. Furthermore, their ROS scavenging performance is investigated by in vitro evaluation of radical removal. Among the three nanoparticles, mesoporous PDA is demonstrated to have the highest scavenging capability, mainly due to its specific surface area. Finally, the study on three in vivo inflammation models is constructed. The results confirm that mesoporous PDA is the most potent scavenger of ROS and more effective in reducing reperfusion injury, improving renal function, and preventing periodontitis progression, respectively. Together with the good biosafety and biocompatibility profiles, PDA nanoparticles, mesoporous PDA in particular, can be a promising avenue of ROS scavenging in fight against the inflammatory diseases.

Regulation of Photophysical Behaviors in Hyperbranched Aggregation-Induced Emission Polymers for Reactive Oxygen Species Scavenging

“…Next, the cellular ROS scavenging capability of HBPSi-Met was tested. Initially, a cellular lesion model was established by incubating NCM460 cells with 1 μg/mL lipopolysaccharide (LPS) for 24 h. , Then, live/dead staining was conducted to test the ROS scavenging ability of HBPSi-Met, in which the red and green fluorescence represented dead cells and live cells, respectively. As depicted in Figure I, both red and green fluorescence were simultaneously monitored in the buffer treated LPS damage cells, attributing to the oxidative stress-induced cell damage by LPS activation.…”

Innovative Bio‐based Hydrogel Microspheres Micro‐Cage for Neutrophil Extracellular Traps Scavenging in Diabetic Wound Healing

“…[ 25 ] PDA, rich in reducing groups like phenol and catechol, exhibits excellent redox capacity and serves as an effective ROS scavenger by donating free radical electrons. [ 26 ] In particular, mPDA NPs, known for their enhanced active site accessibility, have garnered considerable attention in this context. [ 27 ]…”

Erythrocyte-Like Mesoporous PDA@CeO2 Nanozyme with Dual Drugs for Periodontitis Treatment

“…Besides, PDA-based nanocomposites have a wide variety of structures, including core–shell, double-sided, hollow, and mesoporous structure, − especially the erythrocyte-like mesoporous nanobowl, which provides not only sufficient space for drug loading but also more conducive to the uptake of the drug-carrying system by cells . In addition, Zheng reported that the mesoporous PDA has the highest ROS-scavenging capability . Inspired by this, the ultrasmall CeO 2 NPs with multienzyme activity can be grown in situ on nonenzyme activity mPDA NBs due to the chelating property of mPDA for effective ROS elimination.…”

“…38 In addition, Zheng reported that the mesoporous PDA has the highest ROS-scavenging capability. 39 Inspired by this, the ultrasmall CeO 2 NPs with multienzyme activity can be grown in situ on nonenzyme activity mPDA NBs due to the chelating property of mPDA for effective ROS elimination. In addition, due to the large internal and external surfaces and pores, different anti-inflammatory drugs can be loaded into the mPDA NBs.…”