Evaluation of Rat Alveolar Bone Response to Angelus MTA or Experimental Light-cured Mineral Trioxide Aggregate Using Fluorochromes

Gomes‐Filho, João Eduardo; Costa, Mariana Machado Teixeira de Moraes; Cintra, Luciano Tavares Ângelo; Duarte, Paulo Carvalho Tobias; Takamiya, Aline Satie; Lodi, Carolina Simonetti; Bernabé, Pedro Felício Estrada

doi:10.1016/j.joen.2010.11.005

Cited by 30 publications

(33 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…There is evidence that MTA promotes a favorable response in osseous environment, with direct bone apposition 30 . It was demonstrated that MTA induces bone morphogenetic protein 2 expression and calcification in human periodontal ligament cells 20 and stimulating human gingival fibroblasts to produce BMP-2 14 , is able to promote a favorable formation of mineralized tissue in rat alveolar sockets 15 , being characterized by mild inflammatory response and complete bone healing 16 . MTA surfaces also support osteoblast cell attachment, matrix synthesis and RunX2 expression which are essential for osteogenesis 24 .…”

Section: Introductionmentioning

confidence: 99%

Histological and immunohistochemical evaluation of biphasic calcium phosphate and a mineral trioxide aggregate for bone healing in rat calvaria

Silva

Kim

Luczyszyn

et al. 2015

International Journal of Oral and Maxillofacial Surgery

View full text Add to dashboard Cite

Eighty Wistar rats were divided into 4 groups and examined over a period of 2 or 8 weeks. First, a surgical defect was created in the calvaria using a 6 mm diameter trephine drill. The cavity was then treated with BCP (biphasic calcium phosphate), MTA, or the combination of the two. In the control, the cavity was allowed to clot. After 2 or 8 weeks, animals were sacrificed and the bones were subjected to a histological evaluation and immunohistochemical staining to evaluate areas of new osteoid tissue and new bone tissue as well as the percentage of labeled cells that utilize anti-BMPR1B antibodies. Twoway ANOVA showed statistical significant diference in all dependent variable (area of new osteoid tissue; area of new bone and % of immunostainning) within group p=0.00000, time p=0.00000 and with the interaction of both p=0.0000. MTA group at 8 weeks showed the highest amount of osteoid tissue (6.25 ± 0.30 mm 2 ). The same group also exhibited the highest amount of bone tissue formation (3.98 ± 0.14 mm 2 ). In contrast, both 2-week MTA samples (40.73 ± 2.50%) and 2-week BCP+MTA samples (40.30 ± 1.81%) exhibited the highest percentage of stained cells. Best results in terms of the area of osteoid and bone tissue formation and the percentage of BMPR1B markings were observed for the MTA group, confirming that the combination of BCP and MTA does not result in a significant improvement.

show abstract

Section: Introductionmentioning

confidence: 99%

Histological and immunohistochemical evaluation of biphasic calcium phosphate and a mineral trioxide aggregate for bone healing in rat calvaria

Silva

Kim

Luczyszyn

et al. 2015

International Journal of Oral and Maxillofacial Surgery

View full text Add to dashboard Cite

show abstract

“…The distance between the margins of the bone cavity determines the type of cells that first migrate to the defect site, and consequently, the tissue that is formed (6). Fibrous scar formation may occur in cases of apical surgery with root exposure.…”

Section: Discussionmentioning

confidence: 99%

“…It has also demonstrated adequate sealing ability to avoid penetration by microorganisms and their byproducts (4), biocompatibility and adequate solubility properties (3,5), and dimensional stability and radiopacity (5). In addition, in vivo studies have shown that MTA induces the formation of mineralized tissues such as cementum-like tissue (3,5,6).…”

Section: Introductionmentioning

confidence: 99%

“…In the 1980's, the average rate increased to 50% and the size of the lesions diminished in more than 25% of cases (9). However, the success rate of apical surgery remains less than 50% in cases of endoperiodontal lesions (6). The relatively low success rate of apical surgery is directly associated with the variety of factors that can influence the healing process in the periapical region (7).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Root Reconstructed with Mineral Trioxide Aggregate and Guided Tissue Regeneration in Apical Surgery: A 5-year Follow-up

et al. 2013

Self Cite

View full text Add to dashboard Cite

Apical surgery should be considered as the last treatment option and employed when conventional endodontic treatment does not provide the expected result. In teeth undergoing apical surgery, the type of retrograde filling material is one of the factors interfering with the repair of periapical tissues. The material in intimate contact with the periapical tissues plays a fundamental role in the repair process. Several materials have been studied and indicated for use in apical surgery procedures, but the mineral trioxide aggregate (MTA) is still the most frequently used one. Guided tissue regeneration (GTR) techniques have been proposed as an adjunct to apical surgery to enhance bone healing. Here is reported a clinical case in which apical surgery was performed in conjunction with MTA-based root reconstruction of the maxillary right second incisor. After the apical surgery, a root-end cavity was prepared at the vestibular face of the involved tooth and filled with MTA. A bovine bone graft and a cortical collagen membrane were placed on the bone defect. After 5 years, clinical and radiographic assessments showed that the treatment was successful. It may be concluded that MTA presents favorable characteristics in adverse conditions and can be used in conjunction with GTR in cases involving root reconstruction.

show abstract

“…262 When placed into bone, the initial inflammation elicited by MTA decreases over time. 261,[266][267][268][269][270][271] The trend seen in studies of this type is that there is moderate inflammation at 7 days, mild inflammation at 30 days, and no inflammation from 60 days onward.…”

Section: Cytotoxicity (Iso 10993-3)mentioning

confidence: 97%

Mineral Trioxide Aggregate: A review of clinical usage and investigations of the effects of modifying particle size

Ha¹

View full text Add to dashboard Cite

show abstract

Evaluation of Rat Alveolar Bone Response to Angelus MTA or Experimental Light-cured Mineral Trioxide Aggregate Using Fluorochromes

Cited by 30 publications

References 28 publications

Histological and immunohistochemical evaluation of biphasic calcium phosphate and a mineral trioxide aggregate for bone healing in rat calvaria

Histological and immunohistochemical evaluation of biphasic calcium phosphate and a mineral trioxide aggregate for bone healing in rat calvaria

Root Reconstructed with Mineral Trioxide Aggregate and Guided Tissue Regeneration in Apical Surgery: A 5-year Follow-up

Mineral Trioxide Aggregate: A review of clinical usage and investigations of the effects of modifying particle size

Contact Info

Product

Resources

About