Evaluation of quantitative real-time PCR and Platelia galactomannan assays for the diagnosis of disseminated Talaromyces marneffei infection

Li, Xinlei; Zheng, Yanqing; Wu, Fengyao; Mo, Dongdong; Liang, Gang; Yan, Rufan; Khader, Jazeer A.; Wu, Nianning; Cao, Cunwei

doi:10.1093/mmy/myz052

Cited by 24 publications

(35 citation statements)

References 25 publications

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“…The GM assay yielded a higher diagnostic rate than did blood culture (72.5% vs . 66%), consistent with a previous study [25]. A recent study using the Mp1p immunoassay showed that it has superior sensitivity to blood culture in the diagnosis of talaromycosis [27].…”

Section: Discussionsupporting

confidence: 90%

“…However, only 44.5% of the patients in this study had skin lesions, demonstrating the importance of blood cultures for diagnosis. The prevalence of skin lesions in our study is similar to that reported in northern Thailand (40.7%) and in eastern China (43.8%) [18,19,25]. However, it is lower than the prevalence reported in Vietnam (72–83%) [18].…”

Section: Discussionsupporting

confidence: 86%

“…The overall rates of poor outcome and mortality at discharge in our cohort were 14% and 8.0%, respectively, lower than the mortality rates of 17-28% reported in previous studies [10,17,25]. This lower mortality can be attributed to our more aggressive diagnostic strategy with the routine use of bone marrow culture and GM antigen testing, which improves diagnostic yield and facilitates earlier diagnosis and treatment, respectively.…”

Section: Discussioncontrasting

confidence: 65%

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Clinical epidemiology and outcome of HIV‐associated talaromycosis in Guangdong, China, during 2011–2017

Ren

Cai

et al. 2020

HIV Medicine

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Objectives Talaromycosis is an invasive mycosis endemic to Southeast Asia. This study aimed to investigate the epidemiology, clinical features and prognostic factors of HIV‐associated talaromycosis in Guangdong, China. Methods We retrospectively evaluated HIV patients hospitalized with histopathology‐ or culture‐confirmed talaromycosis between 2011 and 2017. Factors associated with poor prognosis were identified using logistic regression. Results Overall, 1079 patients with HIV‐associated talaromycosis were evaluated. Both the number and prevalence of talaromycosis among HIV admissions increased from 125 and 15.7% in 2011 to 253 and 18.8% in 2017, respectively, reflecting the increase in HIV admissions. Annual admissions peaked during the rainy season between March and August. Common clinical manifestations included fever (85.6%), peripheral lymphadenopathy (72.3%), respiratory symptoms (60.8%), weight loss (49.8%), skin lesions (44.5%) and gastrointestinal symptoms (44.3%). Common laboratory abnormalities were hypoalbuminaemia (98.6%), anaemia (95.6%), elevated aspartate aminotransferase level (AST) (76.9%), elevated alkaline phosphatase level (55.8%) and thrombocytopenia (53.7%). The median CD4 count was 9 cells/μL. Talaromyces marneffei was isolated from blood and bone marrow cultures of 66.6% and 74.5% of patients, respectively. The rate increased to 86.6% when both cultures were performed concurrently. At discharge, 14% of patients showed worsening conditions or died. Leucocytosis, thrombocytopenia, elevated AST, total bilirubin, creatinine and azole monotherapy independently predicted poor prognosis. Conclusions The incidence of HIV‐associated talaromycosis has increased in Guangdong with the high HIV burden in China. Skin lesions were seen in less than half of patients. Induction therapy with azole alone is associated with higher mortality. Findings from this study should help to improve treatment of the disease.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionsupporting

confidence: 86%

Section: Discussioncontrasting

confidence: 65%

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Clinical epidemiology and outcome of HIV‐associated talaromycosis in Guangdong, China, during 2011–2017

Ren

Cai

et al. 2020

HIV Medicine

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“…Исследования показали, что выявление GM в сыворотке перспективно для диагностики ТМ у ВИЧ-инфицированных [65]. Li et al [66] оценили эффективность метода выявления GM-антигена у больных ТМ, как инфицированных, так и неинфицированных ВИЧ. Они показали, что средние индексы оптической плотности -optical density (OD) были значительно выше у больных ТМ с фунгемией, чем без неё и у ВИЧ-инфицированных больных, по сравнению с неинфицированными.…”

Section: характеристика возбудителяunclassified

“…Чувствительность и специфичность метода при исследовании образцов плазмы от больных таларомикозом, составили 70,4% и 100% соответственно. Li et al [66] сконструировали TaqMan флуоресцентный зонд и праймеры с высокоспецифичными для T. marneffei регионами ITS1-5,8S-ITS2 рДНК, которые ранее не использовались. При испытании с образцами сывороток больных различными инвазивными микозами подтверждена специфичность метода.…”

Section: характеристика возбудителяunclassified

Talaromycosis (penicilliosis): characteristics of ethiological agent and laboratory diagnosis

Lipnitsky¹,

Половец²,

Surkova³

et al. 2019

Immunopathol Allergol Infectol

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ФКУЗ Волгоградский научноисследовательский противочумный институт Федеральной службы по надзору в сфере защиты прав потребителей и благополучия человека, Волгоград, Россия Talaromycosis (penicilliosis): characteristics of ethiological agent and laboratory diagnosis

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Clinical features of patients with talaromycosis marneffei and microbiological characteristics of the causative strains

Peng

Shi

Zheng

et al. 2022

Clinical Laboratory Analysis

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Background Talaromyces marneffei (T. marneffei) is a temperature‐dependent dimorphic fungus that is mainly prevalent in Southeast Asia and South China and often causes disseminated life‐threatening infections. This study aimed to investigate the clinical features and improve the early diagnosis of talaromycosis marneffei in nonendemic areas. Methods We retrospectively analyzed the medical records of six cases of T. marneffei infection. We describe the clinical manifestations, laboratory tests, and imaging manifestations of the six patients. Results Talaromyces marneffei infection was confirmed by sputum culture, blood culture, tissue biopsy, and metagenomic next‐generation sequencing (mNGS). In this study, there were five disseminated‐type patients and two HIV patients. One patient died within 24 h, and the others demonstrated considerable improvement after definitive diagnosis. Conclusions Due to the lack of significant clinical presentations of talaromycosis marneffei, many cases may be easily misdiagnosed in nonendemic areas. It is particularly important to analyze the imaging manifestations and laboratory findings of infected patients. With the rapid development of molecular biology, mNGS may be a rapid and effective diagnostic method.

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Evaluation of quantitative real-time PCR and Platelia galactomannan assays for the diagnosis of disseminated Talaromyces marneffei infection

Cited by 24 publications

References 25 publications

Clinical epidemiology and outcome of HIV‐associated talaromycosis in Guangdong, China, during 2011–2017

Clinical epidemiology and outcome of HIV‐associated talaromycosis in Guangdong, China, during 2011–2017

Talaromycosis (penicilliosis): characteristics of ethiological agent and laboratory diagnosis

Clinical features of patients with talaromycosis marneffei and microbiological characteristics of the causative strains

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