Evaluation of quality and gene expression of goat embryos produced in vivo and in vitro after cryopreservation

Ferreira-Silva, José Carlos; Silva, Roberta Lane Oliveira; Vieira, Joane Isis Travassos; Silva, Jéssica Barboza; Tavares, Lethicia Souza; Silva, Fabiana Aparecida Cavalcante; Pena, Elton Pedro Nunes; Chaves, Maiana Silva; Moura, Marcelo Tigre; Calsa, Tercílio; Benko‐Iseppon, Ana Maria; Freitas, V. J. F.; Oliveira, Marcos Antônio Lemos

doi:10.1016/j.cryobiol.2021.04.008

Cited by 3 publications

(1 citation statement)

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“…Excessive ROS causes disruption in mitochondrial potential and depletion of intracellular ATP in embryos [98]. Cryopreservation causes oxidative stress and impaired energy homeostasis possibly due to mitochondrial dysfunction [99,100]. On the other hand, the freeze/thaw manipulations have been shown to reduce the amount of mutated mtDNA [6].…”

Section: Effect Of Vitrification On Mitochondrial Functionmentioning

confidence: 99%

Current understanding of the pathogenesis of placenta accreta spectrum disorder with focus on mitochondrial function

Kobayashi,

Matsubara,

Yoshimoto

et al. 2024

J of Obstet and Gynaecol

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AimThe refinement of assisted reproductive technology, including the development of cryopreservation techniques (vitrification) and ovarian stimulation protocols, makes frozen embryo transfer (FET) an alternative to fresh ET and has contributed to the success of assisted reproductive technology. Compared with fresh ET cycles, FET cycles were associated with better in vitro fertilization outcomes; however, the occurrence of pregnancy‐induced hypertension, preeclampsia, and placenta accreta spectrum (PAS) was higher in FET cycles. PAS has been increasing steadily in incidence as a life‐threatening condition along with cesarean rates worldwide. In this review, we summarize the current understanding of the pathogenesis of PAS and discuss future research directions.MethodsA literature search was performed in the PubMed and Google Scholar databases.ResultsRisk factors associated with PAS incidence include a primary defect of the decidua basalis or scar dehiscence, aberrant vascular remodeling, and abnormally invasive trophoblasts, or a combination thereof. Freezing, thawing, and hormone replacement manipulations have been shown to affect multiple cellular pathways, including cell proliferation, invasion, epithelial‐to‐mesenchymal transition (EMT), and mitochondrial function. Molecules involved in abnormal migration and EMT of extravillous trophoblast cells are beginning to be identified in PAS placentas. Many of these molecules were also found to be involved in mitochondrial biogenesis and dynamics.ConclusionThe etiology of PAS may be a multifactorial genesis with intrinsic predisposition (e.g., placental abnormalities) and certain environmental factors (e.g., defective decidua) as triggers for its development. A distinctive feature of this review is its focus on the potential factors linking mitochondrial function to PAS development.

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Section: Effect Of Vitrification On Mitochondrial Functionmentioning

confidence: 99%

Current understanding of the pathogenesis of placenta accreta spectrum disorder with focus on mitochondrial function

Kobayashi,

Matsubara,

Yoshimoto

et al. 2024

J of Obstet and Gynaecol

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Expression profile of viability and stress response genes as a result of resveratrol supplementation in vitrified and in vitro produced cattle embryos

Saber,

Ibrahim,

Mahmoud

et al. 2024

Mol Biol Rep

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In vitro embryo production in small ruminants: what is still missing?

Souza-Fabjan,

Leal,

Monteiro

et al. 2023

Anim. Reprod.

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In vitro embryo production (IVEP) is an extremely important tool for genetic improvement in livestock and it is the biotechnology that has grown the most recently. However, multiple ovulation followed by embryo transfer is still considered the leading biotechnology for embryo production in small ruminants. This review aimed to identify what is still missing for more efficient diffusion of IVEP in small ruminants, going through the IVEP steps and highlighting the main factors affecting the outcomes. Oocyte quality is essential for the success of IVEP and an aspect to be considered in small ruminants is their reproductive seasonality and strategies to mitigate the effect of season. The logistics for oocyte collection from live females is more complex than in cattle, and tools to simplify this collection system and/or to promote an alternative way of recovering oocytes may be an important point in this scenario. The heterogeneity of oocytes collected from growing follicles in live females or from ovaries collected from abattoirs remains a challenge, and there is a demand to standardize/homogenize the hormonal stimulatory protocols and IVM protocols for each source of oocytes. The use of sexed semen is technically possible, however the low market demand associated with the high costs of the sexing process prevents the routine use of this technique, but its higher availability is an important aspect aiming for greater dissemination of IVEP. New noninvasive approaches for embryo selection are key factors since the selection for transfer or cryopreservation is another difficulty faced among laboratories. Embryo selection is based on morphological traits, although these are not necessarily reliable in predicting pregnancy. Several issues described in this review must be considered by researchers in other to promote the diffusion of IVEP in small ruminants.

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Evaluation of quality and gene expression of goat embryos produced in vivo and in vitro after cryopreservation

Cited by 3 publications

References 97 publications

Current understanding of the pathogenesis of placenta accreta spectrum disorder with focus on mitochondrial function

Current understanding of the pathogenesis of placenta accreta spectrum disorder with focus on mitochondrial function

Expression profile of viability and stress response genes as a result of resveratrol supplementation in vitrified and in vitro produced cattle embryos

In vitro embryo production in small ruminants: what is still missing?

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