Evaluation of Pseudomonas aeruginosa biofilm formation using piezoelectric tuning forks mass sensors

Waszczuk, K.; Guła, Grzegorz; Świątkowski, Michał; Olszewski, J.; Drulis-Kawa, Zuzanna; Gutowicz, Jan; Gotszalk, Teodor

doi:10.1016/j.proeng.2010.09.234

Cited by 10 publications

(5 citation statements)

References 6 publications

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“…For either alive or dead PAO1 cell, the thickness of the initial deposited layer is ∼1.5 μm, that is, 1−2 layers of the PAO1 cells (see Figure S3 for the morphology of single PAO1 cells). 26,27 The slope reflects the growth rate of the biofilm. Within the first 2 h, the PAO1 colonies upon dead bacteria grow a little faster than those upon alive bacteria.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

Investigation of Formation of Bacterial Biofilm upon Dead Siblings

et al. 2018

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Biocides can effectively kill bacteria; however, whether the dead bacterial cells left on the surface influence the later growth of biofilm is unknown. In this study, we have cultured Pseudomonas aeruginosa (PAO1) biofilm on their dead siblings and have investigated their evolution by using magnetic force modulation atomic force microscopy (MF-AFM). The time dependence of the biofilm thickness indicates that the deposited dead siblings can slow down the growth of PAO1 biofilm. The biofilm growing on dead bacteria layers is softer in comparison with those upon alive siblings, as reflected by the static elastic modulus ( E) and dynamic stiffness ( k) scaled to the disturbing frequency ( f) as k = k f, where k is the scaling factor and γ is the power-law exponent. We reveal that the smaller population instead of the variation of extracellular polymeric substances (EPS) within the biofilm upon the dead siblings is responsible for the softer biofilm. The present study provides a better understanding of the biofilm formation, thus, making it significant for designing antimicrobial medical materials and antifouling coatings.

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Section: ■ Results and Discussionmentioning

confidence: 99%

Investigation of Formation of Bacterial Biofilm upon Dead Siblings

et al. 2018

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“…The experimental setup is shown in Figure 5, and results are shown in Figure 6. Table I shows a comparison of the sensor in terms of operational range and detection limit with sensors developed by other authors (Waszczuk et al, 2010(Waszczuk et al, , 2012Pang et al, 2007Pang et al, , 2008He and Liu, 2004;Kim et al, 2004). Importantly, the sensor developed by the authors is reusable and shows excellent repeatability; other sensors in this area rely upon substrates or other materials (e.g.…”

Section: Real-time Microwave Based Monitoring Of Pseudomonas Aeruginosamentioning

confidence: 99%

“…Glucose biosensors can also be based on electrochemical principle of detection that employ enzymes for molecular recognition, as well as the optical, piezoelectrical, thermal, and mechanical principles (Tseng et al, 2004;Subramanian et al, 2002;Choudhury et al, 2004;Bababjanyan et al, 2010). An electromagnetic coupling approach for indirect measurement of glucose concentration in sodium chloride and Ringer-lactate solutions, which have Waszczuk et al (2010Waszczuk et al ( , 2012 0.6 £ 10 2 3 -0.6 £ 10 3 - Pang et al (2007Pang et al ( , 2008 0.6 £ 10 2 5 -0.6 0.6 £ 10 2 5 He and Liu (2004) 1.7 £ 10 2 3 -1.7 £ 10 2 1 1.7 £ 10 2 4 Kim et al (2004) 78 £ 10 2 3 -0.78 -Microwave sensor 25 £ 10 2 3 -1.0 25 £ 10 2 3…”

Section: Non-invasive Glucose Monitoring Using Microwavesmentioning

confidence: 99%

Microwave sensors for the non-invasive monitoring of industrial and medical applications

2014

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If you would like to write for this, or any other Emerald publication, then please use our Emerald for Authors service information about how to choose which publication to write for and submission guidelines are available for all. Please visit www.emeraldinsight.com/authors for more information. About Emerald www.emeraldinsight.comEmerald is a global publisher linking research and practice to the benefit of society. The company manages a portfolio of more than 290 journals and over 2,350 books and book series volumes, as well as providing an extensive range of online products and additional customer resources and services.Emerald is both COUNTER 4 and TRANSFER compliant. The organization is a partner of the Committee on Publication Ethics (COPE) and also works with Portico and the LOCKSS initiative for digital archive preservation. AbstractPurpose -This paper aims to discuss the general principles behind the microwave sensing and demonstrates the potential of cavity microwave resonator device in real-time monitoring for: environmental monitoring with the focus on wastewater pollution, a system for oil/gas/water content evaluation in a dynamic pipeline, a system for real-time determination of bacteria concentration and a method for non-invasive glucose determination. Design/methodology/approach -Microwave sensing is a rapidly developing technology which has been successfully used for various industrial applications including water level measurements, material moisture content, in construction industry for non-invasive evaluation of structures and even in the healthcare industry for non-invasive real-time monitoring of glucose in diabetic patients. Novel microwave cavities designed and tested for specific applications are presented. Findings -The paper provides experimental results of testing the novel microwave sensing systems in a range of industrial and healthcare applications and discusses the potential of these systems for real-time monitoring of processes and parameters.Research limitations/implications -The concept of real-time microwave sensing was successfully tested, but further experiments are required to account for possible interference mechanisms before it can be used commercially on a large-scale. Practical implications -It is suggested that a novel approach to wastewater monitoring, namely using specially designed microwave cavity sensors, could lead to a successful development of an advanced platform capable of providing for a real-time detection of water content with superior sensitivity. Also, a system for real-time multiphase fluid composition monitoring is reported, which is essential for sustainable oil industry operation. Originality/value -The paper illustrated the potential of microwave sensing as a real-time monitoring platform for a broad spectrum of commercial applications, with a focus on system developed by the authors, namely, for the monitoring of a multiphase fluid flow in a dynamic oil pipeline, for realtime monitoring of nutrients concentration in wastewater and for healthcare ind...

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“…On the other hand, most probably in several seconds, output of these devices will have been drifting because of several factors like temperature and humidity. [19,22,[28][29][30][31]].…”

Section: Introductionmentioning

confidence: 99%

Design of a portable and low-cost mass-sensitive sensor with the capability ofmeasurements on various frequency quartz tuning forks

Ünal¹,

Kocum²,

Serdaroğlu³

2019

Turk J Elec Eng & Comp Sci

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Recently, sensor and biosensor applications have become widespread and are now significant tools in the biomedical field and other areas. Since quartz tuning fork (QTF) resonance frequency depends on the mass adsorbed to its prongs, it is generally used to measure minor mass change and detect target analyte in picogram levels. This study is undertaken to design and fabricate a sensor device for the measurement of QTF transducers. When QTF sensor studies were investigated, it was found that explanations on the details of instrumentation part were limited, and in addition, there was no compact commercial products. In this study, a novel, low-cost, portable quartz tuning fork sensor device with ability to work with any kind of display is presented in detail. Moreover, the effect of the ambient temperature during the measurements was checked. Six other QTF transducer types, 32, 32.768, 40, 65.536, 75, and 100 kHz, have been studied as the other novel part of the research. As a result, a QTF sensor device was fabricated that has further advanced features when compared with its alternatives and works with a more accurate measurement method. Owing to this advancement, using biosensor/chemical sensor which consists of QTF transducers with features that can take sensitive analyte measurement in picogram level will be able to spread.

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Evaluation of Pseudomonas aeruginosa biofilm formation using piezoelectric tuning forks mass sensors

Cited by 10 publications

References 6 publications

Investigation of Formation of Bacterial Biofilm upon Dead Siblings

Investigation of Formation of Bacterial Biofilm upon Dead Siblings

Microwave sensors for the non-invasive monitoring of industrial and medical applications

Design of a portable and low-cost mass-sensitive sensor with the capability ofmeasurements on various frequency quartz tuning forks

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