Evaluation of Protein Identification and Quantification by the diaPASEF Method on timsTOF SCP
Ju Wang,
Haiyan Tan,
Yingxue Fu
et al.
Abstract:Accurate and precise quantification is crucial in modern proteomics, particularly in the context of exploring low-amount samples. While the innovative 4D-data-independent acquisition (DIA) quantitative proteomics facilitated by timsTOF mass spectrometers gives enhanced sensitivity and selectivity for protein identification, the diaPASEF (parallel accumulation-serial fragmentation combined with data-independent acquisition) parameters have not been systematically optimized, and a comprehensive evaluation of the… Show more
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