Evaluation of protective effect of DNA vaccination with genes encoding antigens GRA4 and SAG1 associated with GM-CSF plasmid, against acute, chronical and congenital toxoplasmosis in mice

Mévélec, Marie-Noëlle; Bout, Daniel; Desolme, Benoît; Marchand, Hervé; Magné, Rémy; Bruneel, Odile; Buzoni‐Gatel, Dominique

doi:10.1016/j.vaccine.2005.04.025

Cited by 91 publications

(58 citation statements)

References 40 publications

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“…These results are consistent with the study by Mévélec et al(2005) in which co-inoculation of pGRA4 and pSAG1mut with pGM-CSF reduced mortality of susceptible C57BL/6 mice upon oral challenge with cysts of the 76K type II strain. In addition, Martin et al(2004) observed that the challenge of recombinant GRA4 protein or recombinant GRA4 and ROP2 proteins vaccinated C57BL/6 and C3H mice with ME49 cysts resulted in fewer brain cysts than the controls, whereas vaccination with recombinant ROP2 protein alone only conferred protection to C3H mice.…”

Section: Discussionsupporting

confidence: 92%

“…Administration of a plasmid encoding the granulocyte macrophage colony-stimulating factor (pGM-CSF) enhanced the protection induced by immunization with plasmid encoding the protein MIC3 (Ismael et al, 2003). Co-inoculation of plasmids expressing GRA4 (pGRA4) and SAG1 (pSAG1mut) with pGM-CSF reduced mortality of susceptible C57BL/6 mice upon oral challenge with cysts of the 76K type II strain (Mévélec et al, 2005). CTXA2/B as a genetic adjuvant enhanced the magnitude of immune responses as well as increased survival rate in mice infected with the lethal RH tachyzoites (Cong et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

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Protective effect of DNA-mediated immunization with liposome-encapsulated GRA4 against infection of Toxoplasma gondii

Rui

Tong

et al. 2009

J. Zhejiang Univ. Sci. B

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Abstract:The dense granule protein 4 (GRA4) is a granular protein from Toxoplasma gondii, and is a candidate for vaccination against this parasite. In this study, the plasmid pcDNA3.1-GRA4 (pGRA4), encoding for the GRA4 antigen, was incorporated by the dehydration-rehydration method into liposomes composed of 16 mmol/L egg phosphatidylcholine (PC), 8 mmol/L dioleoyl phosphatidylethanolamine (DOPE), and 4 mmol/L 1,2-diodeoyl-3-(trimethylammonium) propane (DOTAP). C57BL/6 mice and BALB/c mice were immunized intramuscularly three times with liposome-encapsulated pGRA4 to determine whether DNA immunization could elicit a protective immune response to T. gondii. Enzyme-linked immunosorbent assay (ELISA) of sera from immunized mice showed that liposome-encapsulated pGRA4 generated high levels of IgG antibodies to GRA4. Production of primary interferon (IFN)-γ and interleukin (IL)-2 in GRA4-stimulated splenocytes from vaccinated mice suggested a modulated Th1-type response. 72.7% of C57BL/6 mice immunized with liposome-encapsulated pGRA4 survived the challenge with 80 tissue cysts of ME49 strain, whereas C57BL/6 mice immunized with pGRA4 had only a survival rate of 54.5%. When immunized BALB/c mice were intraperitoneally challenged with 10 3 tachyzoites of the highly virulent RH strain, the survival time of mice immunized with liposome-encapsulated pGRA4 was markedly longer than that of other groups. Our observations show that liposome-encapsulated pGRA4 enhanced the protective effect against infection of T. gondii.

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Section: Discussionsupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Protective effect of DNA-mediated immunization with liposome-encapsulated GRA4 against infection of Toxoplasma gondii

Rui

Tong

et al. 2009

J. Zhejiang Univ. Sci. B

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“…These results suggest that GRA4 can be a good candidate for a multiantigen anti-T. gondii vaccine based on the use of alum as an adjuvant. A mul-tiantigenic vaccine using SAG1 and GRA4 selected on the basis of previous immunological and immunization studies protects well against the infection in mouse [46]. Mortality of susceptible C57BL/6 mice reduced upon oral challenge with cysts of the 76K type II strain by 62% survival and the protection was further increased by co-inoculation with a plasmid encoding the GM-CSF by 87% survival.…”

Section: Gra4mentioning

confidence: 99%

GRA Proteins of Toxoplasma gondii: Maintenance of Host-Parasite Interactions across the Parasitophorous Vacuolar Membrane

Nam

2009

Korean J Parasitol

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Abstract:The dense granule of Toxoplasma gondii is a secretory vesicular organelle of which the proteins participate in the modification of the parasitophorous vacuole (PV) and PV membrane for the maintenance of intracellular parasitism in almost all nucleated host cells. In this review, the archives on the research of GRA proteins are reviewed on the foci of finding GRA proteins, characterizing molecular aspects, usefulness in diagnostic antigen, and vaccine trials in addition to some functions in host-parasite interactions.

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“…It may be possible to provide complete protection against T. gondii infection by combining ROP8 with other immunogenic proteins as previously described, such as ROP16, ROP18, MIC3, SAG1, and GRA4. 4,7,22,25,26 …”

Section: Discussionmentioning

confidence: 99%

Protective Immune Response in BALB/c Mice Induced by DNA Vaccine of the ROP8 gene of Toxoplasma gondii

Parthasarathy¹,

Fong²,

Kalyanasundaram

et al. 2013

The American Society of Tropical Medicine and Hygiene

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Abstract. Toxoplasmosis in humans and other animals is caused by the protozoan parasite Toxoplasma gondii. During the process of host cell invasion and parasitophorous vacuole formation by the tachyzoites, the parasite secretes Rhoptry protein 8 (ROP8), an apical secretory organelle. Thus, ROP8 is an important protein for the pathogenesis of T. gondii. The ROP8 DNA was constructed into a pVAX-1 vaccine vector and used for immunizing BALB/c mice. Immunized mice developed immune response characterized by significant antibody responses, antigen-specific proliferation of spleen cells, and production of high levels of IFN-γ (816 ± 26.3 pg/mL). Challenge experiments showed significant levels of increase in the survival period (29 days compared with 9 days in control) in ROP8 DNA vaccinated mice after a lethal challenge with T. gondii. Results presented in this study suggest that ROP8 DNA is a promising and potential vaccine candidate against toxoplasmosis.

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Evaluation of protective effect of DNA vaccination with genes encoding antigens GRA4 and SAG1 associated with GM-CSF plasmid, against acute, chronical and congenital toxoplasmosis in mice

Cited by 91 publications

References 40 publications

Protective effect of DNA-mediated immunization with liposome-encapsulated GRA4 against infection of Toxoplasma gondii

Protective effect of DNA-mediated immunization with liposome-encapsulated GRA4 against infection of Toxoplasma gondii

GRA Proteins of Toxoplasma gondii: Maintenance of Host-Parasite Interactions across the Parasitophorous Vacuolar Membrane

Protective Immune Response in BALB/c Mice Induced by DNA Vaccine of the ROP8 gene of Toxoplasma gondii

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