Evaluation of Probiotic Properties and Safety of Enterococcus faecium Isolated From Artisanal Tunisian Meat “Dried Ossban”

Abstract

Enterococcus faecium strains were isolated from an original biotope, artisanal dried Tunisian meat “Dried Ossban,” and evaluated for safety and capacity as probiotics. Gram-positive, catalase negative, and bacteriocin-producing bacteria were screened using selective microbiological media. All isolates were identified by phenotypic and molecular tools. Five E. faecium strains (MZF1, MZF2, MZF3, MZF4, and MZF5) were selected and further assessed for their probiotic properties. They were found to be resistant to the physiological concentrations of bile salts, and the harsh conditions of the gastrointestinal tract, and showed autoaggregation and adhesion ability. All these isolates possess at least one enterocin and could efficiently inhibit the growth of Listeria innocua HPB13. The analysis of their safety profile revealed for almost all the strains the absence of cytotoxicity and virulence determinants, and susceptibility to clinically important antibiotics such as vancomycin. These data suggest that these bacteria, isolated from “Dried Ossban,” do not present a risk to human health, and may be considered as interesting candidates for future use as probiotics and bioprotective cultures for application in the food and/or feed industries.

In vitro Assessment of the Probiotic Properties and Bacteriocinogenic Potential of Pediococcus pentosaceus MZF16 Isolated From Artisanal Tunisian Meat “Dried Ossban”

“…About 40 samples of “Dried Ossban,” obtained from homemade production of fermented meat were sterilely collected from different governorates covering almost all the Tunisian territory, and analyzed in the laboratory (Zommiti et al, 2018 ). LAB strains were isolated from this original biotope so called “Dried Ossban,” a Tunisian traditional dry fermented meat typically prepared from sheep intestine and meat mixed with salt and spices and dried through exposure for several days to sun.…”

“…The MZF16 strain isolated from “Dried Ossban” was identified by analysis of its total proteome, using an Autoflex III Matrix-Assisted Laser Desorption/ Ionization-Time-Of-Flight mass spectrometer (MALDI-TOF MS) (Bruker, Marcy-l'Etoile, France) coupled to the MALDI-Biotyper 3.1 system (Hillion et al, 2013 ; Zommiti et al, 2018 ). The result was expressed as a log (score) value, computed by comparison of the peak list of MZF16 strain with the reference main spectral pattern (MSP) in the database.…”

“…In this context, the aim of the current study was to appraise the probiotic properties and bacteriocinogenic potential of P. pentosaceus MZF16, a new strain isolated from artisanal Tunisian meat called “Dried Ossban,” an original biotope recently cited for the first time in a research investigation led by Zommiti et al ( 2018 ).…”

Host Starvation and Female Sex Influence Enterobacterial ClpB Production: A Possible Link to the Etiology of Eating Disorders

“…Bacterial strains from the fecal microbiota of male and female rats, before and after restriction, were isolated on Luria-Bertani medium and identified by analysis of the total proteome using an Autoflex III Matrix-Assisted Laser Desorption/Ionization-Time-Of-Flight mass spectrometer (MALDI-TOF MS) (Bruker, Marcy-l'Etoile, France) coupled to the MALDI-Biotyper 3.1 system, as previously described [12,13]. Formic acid was used on the bacterial spots as a quick extraction procedure [14], then the MALDI target plate was introduced in the mass spectrometer for measurement and data acquisition.…”

Enterococcus faecalis Is a Better Competitor Than Other Lactic Acid Bacteria in the Initial Colonization of Colon of Healthy Newborn Babies at First Week of Their Life

“…The health beneficial roles and use of Enterococcus strains is a controversial topic. The health benefits of specific strains of Enterococcus faecalis and Enterococcus faecium were characterized ( Hlivak et al, 2005 ; Franz et al, 2011 ; Cebrián et al, 2012 ; Hanchi et al, 2018 ; Zommiti et al, 2018 ). Some strains of E. faecalis and E. faecium and some others strains belonging to the species Enterococcus durans , Enterococcus hirae , Enterococcus lactis , and Enterococcus munditii had the potential to promote health to their hosts ( Nami et al, 2014 ; Pieniz et al, 2014 ; Nami et al, 2015 ; Gupta and Tiwari, 2015 ; Haghshenas et al, 2016 ; van Zyl et al, 2016 ).…”