Evaluation of probiotic Lactobacillus plantarum against foodborne pathogens and its fermentation potential in improving Lolium multiflorum silage quality

Abstract

The objective of this study was to isolate the lactic acid bacteria from fermented silage sample and analyze their antibacterial activities, probiotic properties, and fermentation potential in silage. Eleven lactic acid bacteria (LAB) were selected based on distinct morphologies and preliminary studies. Cell-free supernatant (CFS) was then prepared from the selected strains for antibacterial analysis. L-30 strain and its CFS showed highest inhibition (> 10 mm) against tested foodborne pathogens as compared to other strains. Hereafter, the strain L-30 was named as KCC-30 and used for further studies. KCC-30 can survive in the harsh conditions of GIT such as low pH ( 2) and bile salt environment (oxgal) than standard L. plantarum KACC-91016 (pH 2: 27.2% vs 20.5%; oxgal: 72.3% vs 57.7%, both p < 0.05). In addition, KCC-30 exhibited strong autoaggregation (68.3% vs 51.5%) and co-aggregation (33% vs 23.9%) properties. For silage experiment, KCC-30 treatment did not alter the nutrient profiles of silage. At the same time, KCC-30 treatment increased the lactic acid content of silage as compared to untreated silage (5.55 DM% vs 3.11 DM%). An increase of lactic acid content in the silage is due to higher lactic acid bacteria population in KCC-30 treated silage (15.33 × 10 7 CFU/g vs 7.66 × 10 7 CFU/g) than untreated silage (p < 0.05). Overall data suggested that KCC-30 exhibited strong probiotic potential and improved the quality of Lolium multiflorum silage by increasing the lactic acid level. Therefore, KCC-30 could be considered as potential strain to improve the fermentation quality of L. multiflorum silage.

Probiotic and Triticale Silage Fermentation Potential of Pediococcus pentosaceus and Lactobacillus brevis and Their Impacts on Pathogenic Bacteria

“…Furthermore, fermented silages with LAB showed higher antibacterial activities against a panel of cattle pathogenic bacteria based on inhibitory zones with the agar diffusion method. LAB exhibits significant antimicrobial activities against different pathogenic bacteria [29,50,51]. As we discussed earlier, TC48 and TC50 effectively ferment the triticale silage in both low and high moisture level by increasing lactic acid and reducing pH of silage.…”

Genotypic and Phenotypic Characterization of Bacteriocinogenic Lactic Acid Bacterial Strains for Possible Beneficial, Virulence, and Antibiotic Resistance Traits

“…Auto-aggregation is important for probiotic LAB strains since it is correlated with the adherence capability to epithelial cells (Somashekaraiah et al, 2019, Kong et al, 2020, Reuben et al, 2020. LAB competes with pathogens by preventing them to adhere to the binding sites of intestinal epithelial cells, thus reducing its colonization (Campana et al, 2017, Srisesharam et al, 2018. In our study, the autoaggregation and co-aggregation capacities of these 10 bacteriocinogenic LAB strains were measured (Figures 1 & 2).…”

“…This prevents intestinal surface colonization of pathogenic microorganisms (Campana et al, 2017). The beneficial LAB strain produces antimicrobial substances, thus destroying or eliminating the pathogens (Srisesharam et al, 2018). Coaggregation of these 10 bacteriocinogenic LAB strains was tested using three different indicator strains after 60 min.…”

Anti-Inflammatory Effects of Limosilactobacillus fermentum KGC1601 Isolated from Panax ginseng and Its Probiotic Characteristics

“…Probiotics require sufficient interaction with the host to provide health benefits to the host gut [ 34 ]. Adhesion of the probiotics to the intestinal epithelial cells is essential for producing substances for growth and substances that inhibit pathogenic bacteria growth in the intestines [ 35 , 36 ]. Therefore, the ability to adhere to intestinal epithelial cells is one of the important properties as probiotics.…”