Evaluation of Probiotic and Antimicrobial Potential of Canine Derived–<em>Lactobacilli</em>

Abstract

Antibiotics are commonly used to treat infectious diseases. However, massive and inappropriate antibiotics usage cause many problems including the emergence and spread of antibiotic-resistant bacteria. To avoid this issue, in modern countries the interest of using probiotics in feed supplementation to promote health and prevent or treat intestinal infectious diseases in companion animals like dogs has been increasing. We evaluate the probiotic potential of Lactobacilli isolated from healthy dogs faeces. The isolated Lactobacilli were first confirmed by 16SrRNA sequencing, then in vitro tests were conducted to assess survival potential of Lactobacilli under simulated gastrointestinal conditions and adhesion ability to gut epithelia, effects on epithelial barrier function, anti-inflammatory activities, effects on defensin peptides (beta-defensin 3) and inhibitory effects on common pathogens. Lactobacilli showed considerable potential to survive in simulated gastrointestinal environmental conditions, low pH, high bile salt concentrations along with good adhesion properties with MODE-K cells. Pathogenic bacterial growth and their adhesion to MODE-K cells was significantly inhibited by Lactobacilli. Real-time PCR analyses further demonstrated that L. acidophilus strain AR1 and AR3 inhibit Salmonella-induced proinflammatory cytokines (IL-6, IL-8, 1ꞵ) production and reinforce expression of tight junction protein (occludin). None of the strain induce mRNA expression of beta-defensin 3 in MODE-K cells. Based on in vitro results the L. acidophilus strain AR1 has potential to be supplemented in canine feed. However, further in vivo studies investigating health-promoting effects are awaited.

Formulación probiótica derivada de cepas nativas de perro usada como complemento en el tratamiento de enfermedades gastrointestinales de mascotas.

“…Con respecto al efecto probiótico in vitro, varios estudios sugieren la desregulación de marcadores de respuesta inmune innata inducida como TNF-α e IL-8 y receptores de reconocimiento de patrones (RRP) como NOD-2 y TLR2 están en enfermedades inflamatorias como la enfermedad inflamatoria intestinal canina (EII) (Peiravan et al, 2018;Konstantinidis et al, 2021) y Enteropatía crónica (EC) (Eissa et al, 2019), donde se demostró el efecto regulador de probióticos sobre todos estos factores (Xu et al, 2019b;Zhang et al, 2021;Raheem et al, 2022). En nuestro estudio, los probióticos mostraron una función inmunomoduladora preliminar in vitro, promoviendo un aumento significativo en la expresión de citoquinas proinflamatorias y receptores de reconocimiento de patrones.…”