Evaluation of Precision Xceed® meter for on-site monitoring of blood β-hydroxybutyric acid and glucose concentrations in dairy sheep

Panousis, Nikolaos; Brozos, Christos; Karagiannis, I.; Giadinis, Nektarios D.; Lafi, Shawkat Q.; Kritsepi‐Konstantinou, Maria

doi:10.1016/j.rvsc.2011.06.019

Cited by 73 publications

(81 citation statements)

References 17 publications

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“…In all the occasions, samples were collected 6 -7 h after the morning feeding of the animals. A drop of blood was placed on a strip, which was subsequently inserted into an automated reader (Precision Xceed Meter; Abbott Laboratories, Abbott Park, IL, USA), validated for measurement of β-hydroxybutyrate concentration in sheep blood (Panousis et al, 2012).…”

Section: Samplings and Examinationsmentioning

confidence: 99%

Potential association between trematode infections and development of pregnancy toxaemia in sheep

et al. 2013

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Objective of the work was to study a potential association between trematode infections in pregnant ewes and concentrations β-hydroxybutyrate, which is a ketone body found in animals with pregnancy toxaemia. After administration of a long-acting nematocide, 80 pregnant sheep, infected with trematodes, were allocated as follows; primigravidae ewes in group P-A remained untreated and in group P-B were given netobimin; multigravidae ewes in group M-A remained untreated, in group M-B were given netobimin and in group M-C were given rafoxanide. We collected faecal samples for trematode epg counting and blood samples for measurement of β-hydroxybutyrate concentrations. Mean faecal epg counts of D. dendriticum and F. hepatica decreased significantly after netobimin administration; mean faecal epg counts of F. hepatica, but not of D. dendriticum, decreased significantly after rafoxanide administration. Between P-A and P-B, the difference in mean blood β-hydroxybutyrate concentrations was significant (P = 0.036) immediately after lambing. Between M-A or M-C and M-B, it was significant (P ≤ 0.002) 28 days after trematocide administration and immediately after lambing; between M-A and M-C, no significant difference was evident. Immediately after lambing, mean blood β-hydroxybutyrate concentration in primiparous/multiparous ewes with Dicrocoelium faecal output ≤150 epg was 0.21/0.64 mmol L -1 , respectively, and in primiparous/multiparous ewes with Dicrocoelium faecal output >150 epg was 0.40/0.93 mmol L -1 , respectively (P = 0.704/<0.001, respectively). Mean blood β-hydroxybutyrate concentration in group P-C/M-C ewes with Fasciola faecal output of <16/<30 epg was 0.47/0.68 mmol L -1 , respectively; that in group P-C/M-C ewes with Fasciola faecal output of ≥16/≥30 epg was 0.56/0.85 mmol L -1 , respectively (P = 0.620/0.278, respectively). The results indicate that more trematode-infected adult ewes were found to have increased β-hydroxybutyrate blood concentrations and point out to a potential role of liver trematode .... infections in predisposing adult ewes to pregnancy toxaemia.

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Section: Samplings and Examinationsmentioning

confidence: 99%

Potential association between trematode infections and development of pregnancy toxaemia in sheep

et al. 2013

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“…31,34 Method comparison studies utilizing ovine and caprine blood have identified proportional and constant biases for the meter relative to reference methods for BHB. [35][36][37] Complete characterization of the analytic performance of the meter, including identifying the linear range, would improve the clinical utility of the meter in measuring the BHB in blood from dairy cattle.…”

Section: Introductionmentioning

confidence: 99%

Characterization of the analytic performance of an electrochemical point‐of‐care meter for measuring β‐hydroxybutyrate concentration in blood and plasma from periparturient dairy cattle

Megahed

Hiew

Townsend

et al. 2017

Veterinary Clinical Pathol

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“…A first report using an electronic human BHB meter (MediSense Precision, Abbott, Abingdon, UK) for dairy cows described a high correlation (r 2 = 0.99) with BHB concentrations determined spectrophotometrically (the gold standard laboratory test) and considered the test to be suitable for detecting subclinical ketosis in dairy cows (Jeppesen et al, 2006). An electronic patient-side test for measuring whole-blood BHB concentration (Precision Xtra, Abbott Diabetes Care, Abingdon, UK) was recently validated for use in dairy cows (Iwersen et al, 2009;Mahrt et al, 2014) and sheep (Panousis et al, 2012). The main advantages of the Precision Xtra test include an immediate quantitative measurement with high sensitivity and specificity, without logistical efforts for laboratory analysis (e.g., cooling, centrifugation, or shipping).…”

Section: Introductionmentioning

confidence: 99%

Validation of a calf-side β-hydroxybutyrate test and its utility for estimation of starter intake in dairy calves around weaning

Deelen

Leslie

Steele

et al. 2016

Journal of Dairy Science

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Recent research suggests that circulating β-hydroxybutyrate (BHB) levels may be a meaningful indicator of grain intake and rumen development in pre-ruminant calves. As such, BHB levels may be a surrogate measure of rumen function to contribute to minimal weaning stress during the transition from liquid to solid feed. The primary objective of this study was to determine the optimal cut-point of circulating BHB levels that would be predictive of sufficient grain intake and rumen development for a successful transition from liquid to solid feed at the time of weaning. An additional objective was to validate the Precision Xtra (Abbott Diabetes Care, Abingdon, UK) calf-side test for determination of BHB in whole blood in calves around weaning, as compared with the gold standard laboratory method. A total of 20 Holstein female calves were randomly assigned at birth to be weaned at 6 wk (n=10) or 8 wk (n=10) of age. Milk replacer (mixed at 150 g/L) was offered at 1.2kg/calf per d in 2 meals until a 1-wk step down, when milk meals were reduced by 50% 1 wk before weaning. Daily measurements included the intakes of starter grain, chopped straw, and water. Weekly measurements included body weight and blood BHB, until 70 d of life. To assess digestive tract development, rumen fluid samples were taken before and after weaning (d 35, 49, and 63) and analyzed for ruminal short-chain fatty acids. Whole blood was collected by jugular venipuncture, and BHB was determined by the Precision Xtra test at calf-side. In addition, serum was separated from a clotted sample, frozen, and stored until laboratory analysis was performed. Laboratory BHB results were correlated with both the Precision Xtra test (r=0.95) and starter intake over 1 d (r=0.89), a 3-d average (r=0.90), and a 7-d average (r=0.90). Additionally, laboratory BHB results were associated with total ruminal volatile fatty acids (r=0.82), ruminal butyrate (r=0.77), and body weight (r=0.69). A receiver operating characteristic curve was created to plot the true positive rate against the false positive rate at 10μmol/L BHB intervals to determine the optimal cut-point of circulating BHB that is predictive of an average starter intake of 1,000 g/d over a 3-d period. The optimal combination of sensitivity (95.7%) and specificity (96.1%) was at 100µmol of BHB/L of blood. A value of 0.2 mmol/L on the Precision Xtra test yielded a sensitivity and specificity of 84.0 and 97.2%, respectively, over the 3-d average period of starter intake. These results show considerable promise for use of the Precision Xtra whole blood BHB test in the decision-making process of determining sufficient starter grain intake and rumen development for a successful transition from liquid to solid feed, and indicate that this test conducted at calf-side is highly accurate.

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Evaluation of Precision Xceed® meter for on-site monitoring of blood β-hydroxybutyric acid and glucose concentrations in dairy sheep

Cited by 73 publications

References 17 publications

Potential association between trematode infections and development of pregnancy toxaemia in sheep

Potential association between trematode infections and development of pregnancy toxaemia in sheep

Characterization of the analytic performance of an electrochemical point‐of‐care meter for measuring β‐hydroxybutyrate concentration in blood and plasma from periparturient dairy cattle

Validation of a calf-side β-hydroxybutyrate test and its utility for estimation of starter intake in dairy calves around weaning

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