Evaluation of Precision and Establishment of Reference Ranges for Plasma Thyroxine using a Point-of-Care Analyzer in Healthy Guinea Pigs (Cavia porcellus)

Fredholm, Daniel V.; Cagle, Laura A.; Johnston, Matthew S.

doi:10.1053/j.jepm.2011.11.004

Cited by 17 publications

(3 citation statements)

References 16 publications

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“…We chose to use this analyzer because it provides immediate patient-side results and requires only a small volume of blood. 10 This analyzer has been used before in research in black-tailed prairie dogs both in field 29 and laboratory settings. 7,8,13,18 In addition to enzyme immunoassays, thyroid hormones can be measured by using radioimmunoassays, fluorescence immunoassays, time-resolved fluorescence immunoassays, and chemiluminescent immunoassay.…”

Section: Discussionmentioning

confidence: 99%

“…Reference intervals obtained by using this assay were previously reported for this species, 8 and a full precision and validation study performed on guinea pigs produced excellent results (r i = 0.95; mean coefficient of variation, 4.2% [range for T4 in Guinea pigs, 0 to 15.7], n = 90). 10 In the current study, each T4 rotor was used within 15 min of removal from the refrigerator and immediately after opening its protective pouch. Tests were analyzed immediately after rotor filling and according to the manufacturer directions.…”

Section: Influence Of Isoflurane Anesthesia On Plasmamentioning

confidence: 99%

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Influence of Isoflurane Anesthesia on Plasma Thyroxine Concentrations in Black-tailed Prairie Dogs (Cynomys ludovicianus)

Eshar

Gardhouse

Beaufrère

2018

j am assoc lab anim sci

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Anesthesia can affect measured thyroxine (total T4) concentrations in humans and animals, but its effect in black-tailed prairie dogs (Cynomys ludovicianus) has not yet been studied. We used isoflurane to anesthetize 12 prairie dogs for 60 min. Blood samples were obtained from each animal immediately after anesthesia induction and at 30 and 60 min and used for analysis of plasma T4 concentration. The plasma T4 concentration (mean ± 1 SD) was significantly decreased from baseline (3.49 ± 0.52 μg/dL) at both 30 min (3.24 ± 0.52 μg/dL) and 60 min (3.27 ± 0.65 μg/dL) after induction. Compared with baseline, some of the T4 trends were inconsistent between animals, and individual variability in response was responsible for 86% of the overall variability. Regardless of the observed change under isoflurane anesthesia, all measurements in all prairie dogs and at all time points (2.4 to 4.4 μg/dL) were within the reported normal plasma T4 reference range for this species. In conclusion, isoflurane anesthesia appears to cause a significant but inconsistent reduction in plasma T4 concentrations in black-tailed prairie dogs, but because values remain within normal basal levels, the clinical importance of this effect is likely minimal.

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Section: Discussionmentioning

confidence: 99%

Section: Influence Of Isoflurane Anesthesia On Plasmamentioning

confidence: 99%

Influence of Isoflurane Anesthesia on Plasma Thyroxine Concentrations in Black-tailed Prairie Dogs (Cynomys ludovicianus)

Eshar

Gardhouse

Beaufrère

2018

j am assoc lab anim sci

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“…Reference value by Jensen et al 2013 [ 13 ]; (e) tT4 value obtained by enzyme immunoassay. Reference value by Fredholm et al 2012 [ 14 ]; (f) urine test strip (Combur 10 Test UX, Roche Diagnostics, Mannheim, Germany) measured by an automated analyser (Urisys 1100, Roche Diagnostics, Mannheim, Germany).…”

Section: Figurementioning

confidence: 99%

Case Report of a Satin Guinea Pig with Fibrous Osteodystrophy That Resembles Human Pseudohypoparathyroidism

Gallego

2017

Case Reports in Veterinary Medicine

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A case report of a 2-year-old female satin guinea pig with a history of dental overgrowth and lameness and radiological lesions of fibrous osteodystrophy is presented. The most relevant clinical findings were bone demineralization, high level of parathyroid hormone (PTH), normophosphatemia, normal ionized calcium, and low total thyroxine (tT4) with a normal renal function. Long-term treatment was based on teeth coronal reduction and maintaining a balanced diet. PTH measurement was performed with a kit suitable for rats to test 4 different paired samples of guinea pigs and resulted in similar results for each pair of measurements. Two kits routinely employed in dogs and cats failed in measuring PTH in guinea pig serum samples. The ionized calcium, PTH, and tT4 values, not previously reported in similar cases, were obtained. The determination of tT4 could be useful in the diagnosis of fibrous osteodystrophy in guinea pigs. The observed findings show similarity with human pseudohypoparathyroidism type Ia, a disease caused by an inactivating heterozygous mutation of the stimulatory G protein α subunit from the maternal genome that induces multiple hormone resistance and that courses with a syndrome called Albright hereditary osteodystrophy. Naturally occurring pseudohypoparathyroidism in animals has been reported previously only in a ferret.

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Ancillary Diagnostics

Brandão

2021

Exotic Animal Emergency and Critical Care Medicine

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Evaluation of Precision and Establishment of Reference Ranges for Plasma Thyroxine using a Point-of-Care Analyzer in Healthy Guinea Pigs (Cavia porcellus)

Cited by 17 publications

References 16 publications

Influence of Isoflurane Anesthesia on Plasma Thyroxine Concentrations in Black-tailed Prairie Dogs (Cynomys ludovicianus)

Influence of Isoflurane Anesthesia on Plasma Thyroxine Concentrations in Black-tailed Prairie Dogs (Cynomys ludovicianus)

Case Report of a Satin Guinea Pig with Fibrous Osteodystrophy That Resembles Human Pseudohypoparathyroidism

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