Evaluation of pre-mir-34a rs72631823 single nucleotide polymorphism in triple negative breast cancer: A case-control study

Kalapanida, Despoina; Zagouri, Flora; Gazouli, Maria; Zografos, Εleni; Dimitrakakis, Constantine; Marinopoulos, Spyridon S; Giannos, Aris; Sergentanis, Theodoros N.; Kastritis, Efstathios; Terpos, Evangelos

doi:10.18632/oncotarget.26385

Cited by 6 publications

(5 citation statements)

References 56 publications

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“…conversely, mirnas such as mir-155 are reportedly overexpressed in various types of cancer, and act as oncogenes (44). changes in mirna expression contribute to the initiation and progression of cancer (45,46). The association between mirnas and tumors suggests that mirnas may be altered in patients with gastric cancer (47,48).…”

Section: Discussionmentioning

confidence: 99%

Differential expression profile analysis of cisplatin‑regulated miRNAs in a human gastric cancer cell line

et al. 2019

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cisplatin, one of the most commonly used drugs in combination chemotherapy, is an effective anti-tumor agent widely used for diverse tumor types. Micrornas (mirnas/mirs) are involved in the occurrence, development, diagnosis and treatment of cancer. Therefore, the aim of the current study was to explore whether cisplatin exerts anticancer effects by causing differential expression of mirnas in human gastric cancer cells. The human gastric cancer cell line nci-n87 was cultured with a certain dose of cisplatin and high-throughput sequencing combined with reverse transcription-quantitative polymerase chain reaction (rT-qPcr) was performed to detect cisplatin-regulated mirnas. mirnas upregulated and downregulated following cisplatin exposure were analyzed. High-throughput sequencing revealed 33 upregulated and 16 downregulated miRNAs. A total of five significantly upregulated and five significantly downregulated mirnas were identified by rT-qPcr. The expression levels of hsa-mir-1246 and hsa-mir-892b were consistent with the results obtained from high-throughput sequencing. Gene ontology and Kyoto encyclopedia of Genes and Genomes pathway clustering of cisplatin-regulated mirnas revealed that the mirnas regulated genes involved in several biological processes and signaling pathways. The results obtained in the current study suggested that cisplatin may exert an important anticancer effect in gastric cancer via complex biological processes and signaling pathways.

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Section: Discussionmentioning

confidence: 99%

Differential expression profile analysis of cisplatin‑regulated miRNAs in a human gastric cancer cell line

et al. 2019

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“…The full texts of the remaining 79 records were evaluated and 10 articles were excluded for the following reasons: insufficient reported data ( n : 4) [16,17,18,19], studying other genes or polymorphisms ( n : 3) [20,21,22], not a case-control design ( n : 2) [23,24], and a duplicate study ( n : 1) [25]. Finally, a total of 69 articles remained [26,27,28,29,30,31,32,33,34,35,36,37,38,39,40,41,42,43,44,45,46,47,48,49,50,51,52,53,54,55,56,57,58,59,60,61,62,63,64,65,66,67,68,69,70,71,72,73,74,75,76,77,78,79,80,81,82,83,84,85<...>…”

Section: Resultsmentioning

confidence: 99%

Evidences from a Systematic Review and Meta-Analysis Unveil the Role of MiRNA Polymorphisms in the Predisposition to Female Neoplasms

Bastami

Choupani

Saadatian

et al. 2019

IJMS

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Breast (BCa) and gynecological (GCa) cancers constitute a group of female neoplasms that has a worldwide significant contribution to cancer morbidity and mortality. Evidence suggests that polymorphisms influencing miRNA function can provide useful information towards predicting the risk of female neoplasms. Inconsistent findings in the literature should be detected and resolved to facilitate the genetic screening of miRNA polymorphisms, even during childhood or adolescence, and their use as predictors of future malignancies. This study represents a comprehensive systematic review and meta-analysis of the association between miRNA polymorphisms and the risk of female neoplasms. Meta-analysis was performed by pooling odds-ratios (ORs) and generalized ORs while using a random-effects model for 15 miRNA polymorphisms. The results suggest that miR-146a rs2910164 is implicated in the susceptibility to GCa. Moreover, miR-196a2 rs11614913-T had a moderate protective effect against female neoplasms, especially GCa, in Asians but not in Caucasians. MiR-27a rs895819-G might pose a protective effect against BCa among Caucasians. MiR-499 rs3746444-C may slightly increase the risk of female neoplasms, especially BCa. MiR-124 rs531564-G may be associated with a lower risk of female neoplasms. The current evidences do not support the association of the remaining polymorphisms and the risk of female neoplasms.

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“…It's worth noting that although this limitation above, an essential element of the validity of our study is its agreement with HWE in both study groups, particularly the controls which exclude any genotyping errors or guided sample selection by the authors. Another raised limitation in this study could be related to evaluation of the study variant in FFPE normal colon tissue samples, which, however, is "a very common source for DNA extraction in the studies regarding microRNAs" 47 .…”

Section: Discussionmentioning

confidence: 99%

“…It has been reported that miRNA SNPs might also cause an aberrant function of the miRNA in regulating the putative target genes 44 . Previous researches have shown that MIR34A variants could modulate the susceptibility of individuals to multiple human cancers, including osteosarcoma 45 , colon cancer 46 , and breast cancer 47 . The present results revealed that MIR34A rs2666433 AA and AG genotype carriers were 5.7 and 2.8 more likely to develop cancer than GG carriers.…”

Section: Discussionmentioning

confidence: 99%

Analysis of microRNA-34a expression profile and rs2666433 variant in colorectal cancer: a pilot study

Fawzy

Ibrahiem

AlSel

et al. 2020

Sci Rep

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MicroRNAs (miRNAs) are implicated in every stage of carcinogenesis and play an essential role as genetic biomarkers of cancer. We aimed to evaluate microRNA-34a gene (MIR34A) expression in colorectal cancer (CRC) tissues compared with non-cancer one and to preliminarily explore the association of one related variant to CRC risk. A total of 116 paraffin-embedded colon specimens were enrolled. MiR-34a was quantified by qPCR, and rs2666433 (A/G) genotyping was performed by TaqMan Real-Time PCR. Also, the somatic mutation burden was assessed. MIR34A expression in the CRC specimens was significantly upregulated (median = 21.50, IQR: 7.0–209.2; P = 0.001) relative to the non-cancer tissues. Allele (A) was highly prevalent in CRC tissues represented 0.56 (P < 0.001). AA/AG genotype carriers were 5.7 and 2.8 more likely to develop cancer than GG carriers. Tumor-normal tissue paired analysis revealed genotype concordance in 33 out of 58 tissue samples. Approximately 43% of the specimens showed a tendency for G to A shift. Additionally, a higher frequency of somatic mutation (92%) was observed in adenocarcinoma (P = 0.006). MIR34A expression and gene variant did not show associations with the clinicopathological data. However, G > A somatic mutation carriers had more prolonged DFS and OS. Bioinformatics analysis revealed miR-34a could target 30 genes that are implied in all steps of CRC tumorigenesis. In conclusion, this study confirms MIR34A upregulation in CRC tissues, and its rs2666433 (A/G) variant showed association with CRC and a high somatic mutation rate in cancer tissues. MiR-34a could provide a novel targeted therapy after validation in large-scale studies.

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Evaluation of pre-mir-34a rs72631823 single nucleotide polymorphism in triple negative breast cancer: A case-control study

Cited by 6 publications

References 56 publications

Differential expression profile analysis of cisplatin‑regulated miRNAs in a human gastric cancer cell line

Differential expression profile analysis of cisplatin‑regulated miRNAs in a human gastric cancer cell line

Evidences from a Systematic Review and Meta-Analysis Unveil the Role of MiRNA Polymorphisms in the Predisposition to Female Neoplasms

Analysis of microRNA-34a expression profile and rs2666433 variant in colorectal cancer: a pilot study

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