Evaluation of pre-analytical, demographic, behavioural and metabolic variables on fibrinolysis and haemostasis activation markers utilised to assess hypercoagulability

Abstract

Fibrinolytic variables are predominantly modulated by age, body mass index and blood lipids, while haemostasis activation markers are mainly un-influenced by these factors.

Effect of Estrogen-Progestin Hormonal Replacement Therapy on Blood Coagulation and Fibrinolysis in Postmenopausal Women

“…[37][38][39] Monitoring these markers can identify a hypercoagulable state associated with a statistically significant reduction of AT-III levels, despite remaining within the normal range (higher than 70%). F1+2 levels increased after three months of treatment in all the groups, compared to the initial values.…”

Association of Haemostasis Activation Markers with Thrombophilia and Venous Thromboembolism

“…The levels of these markers remain low also during treatment with warfarin (Elias et al, 1993;Jerkemann et al, 2000; Vižintin-Cuderman et al, to be published). TF does not seem to influence F1+2 and TAT levels either during anticoagulant treatment or after its withdrawal (Cuderman et al, 2007;Vižintin-Cuderman et al, to be published). There are few studies on the utility of these markers in assessing VTE recurrence risk.…”

“…However, they are extremely sensitive to in vitro artefacts. The quality of the sample and the reliability of the result depend on the technique of blood sampling and the experience of the person performing the procedure (Stegnar et al, 2007). Besides blood sampling, the different reagents used as well as the preparation and storage of the samples can influence the results (Greenberg et al 1994;Miller et al, 1995).…”

“…Surprisingly, there are few studies on the association of D-dimer with specific TF defects. Elevated D-dimer was found in carriers of factor V Leiden (Eichinger et al, 1999, Cuderman et al, 2007 and in the presence of antiphospholipid antibodies (Ames et al, 1996).…”

Plasma centrifugation does not influence thrombin-antithrombin and plasmin-antiplasmin levels but determines platelet microparticles count

“…On the other hand, a short centrifugation time influences results of conventional coagulation testing (APTT - activated partial thromboplastin time and fibrinogen concentrations) and the role of residual blood elements (microplatelets, microparticles - MPs) is proposed ( 4 ). The other preanalytical factor, which may significantly influence coagulation testing, is inappropriate sampling by an untrained phlebotomist, which may cause a significant increase in thrombin generation parameters ( 5 ). …”