Evaluation of Pot’s Technique and Sodium Hypochlorite Concentration Technique in Smear Negative Sputum Samples for Increased Sensitivity of Sputum Microscopy

Singhania, Priya; Kaur, Surender; Kaore, Navinchandra M.; Gautam, Shantaram K; Kaore, Shilpa N.; Tayyaba, Anwari

doi:10.7860/jcdr/2019/40119.12599

Cited by 1 publication

(2 citation statements)

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“…The sensitivity and specificity of the pot methods were found to be 100% in comparison to conventional auramine O staining. Previous studies from India had reported similar findings, with 96.8% -100% sensitivity and 91.7% -99.6% specificity (12)(13)(14). In this present study, the smear grading with 3+ AFB bacilli was comparatively less in pot methods than the conventional auramine O method.…”

Section: Discussionsupporting

confidence: 83%

“…There were discrepancies between previous studies that used pot methods. Two studies from India reported an increase in 3+ grading smears using pot methods, whereas another study reported a decrease in 3+ grading smears (12,14,15). These variations could be due to the dilution of sputum samples by adding an equal volume of PhAS-stain solution, and also the quality of sputum used while preparing the smear.…”

Section: Discussionmentioning

confidence: 99%

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Evaluation of phenol ammonium sulfate basic fuchsin and auramine O staining by pot technique for the detection of acid-fast bacilli among patients suspected of pulmonary tuberculosis

Hazra¹,

Krithika²,

Shenoy³

et al. 2022

Biomedicine

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Introduction and Aim: Tuberculosis (TB) remains a major public health concern with its high incidence and mortality. In developing countries, smear microscopy continues to be the primary diagnostic tool for the diagnosis of TB. Sputum samples processing under resource-limited settings could be hazardous, which urges safe and efficient smear microscopy techniques. This study aimed to evaluate the efficacy of two different pot methods: Phenol ammonium sulfate (PhAS) auramine O and PhAS basic fuchsin in comparison to the conventional Auramine O method. Materials and Methods: A prospective cross-sectional study was conducted at the Department of Microbiology with 74 sputum samples. All sputum samples were processed using the Auramine O method PhAS auramine O and PhAS basic fuchsin staining methods. Microsoft Office Excel (Microsoft, Redmond, WA, USA) was used to maintain and analyze all the data. Results: A total of 8 (10.7%) samples were positive for AFB and 67 (89.3%) samples were negative, according to routine auramine O method and pot methods. Pot methods showed a 100% sensitivity and specificity compared to the conventional Auramine O method. In terms of sputum smear grading, the direct smear using auramine O showed better results compared to pot methods. Conclusion: Pot methods like PhAS basic fuchsin and PhAS auramine O are efficient to detect AFB in sputum smears by reducing the risk of laboratory-acquired infections.

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Section: Discussionsupporting

confidence: 83%

Section: Discussionmentioning

confidence: 99%