Evaluation of positive interaction for cell growth between Bifidobacterium adolescentis and Propionibacterium freudenreichii using a co-cultivation system with two microfiltration modules

Kouya, Tomoaki; Ishiyama, Yohei; Tanaka, Takaaki; Taniguchi, Masayuki

doi:10.1016/j.jbiosc.2012.09.005

Cited by 8 publications

(3 citation statements)

References 13 publications

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“…Control mechanisms for cocultures via interspecies interactions (such as quorum sensing and metabolite exchange) have been described ( 76 – 78 ), and dynamic control of these interactions using optogenetics and predictive control strategies ( 36 , 79 ) could enable community maintenance and optimization. Similar optogenetic approaches in monocultures have already enabled significant gains in bioproduction ( 35 , 80 , 81 ). In addition, the spatial control provided by light could allow the formation of sophisticated living biomaterials.…”

Section: Discussionmentioning

confidence: 99%

Optogenetic Tools for Control of Public Goods in Saccharomyces cerevisiae

Morales

Patel

Stewart

et al. 2021

mSphere

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Section: Discussionmentioning

confidence: 99%

Optogenetic Tools for Control of Public Goods in Saccharomyces cerevisiae

Morales

Patel

Stewart

et al. 2021

mSphere

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“…Control mechanisms for cocultures via interspecies interactions (such as quorum sensing and metabolite exchange) have been described 74–76 and dynamic control of these interactions using optogenetics and predictive control strategies 36,77 could enable community maintenance and optimization. Similar optogenetic approaches in monocultures have already enabled significant gains in bioproduction 35,78,79 . In addition, the spatial control provided by light could allow the formation of sophisticated living biomaterials.…”

Section: Discussionmentioning

confidence: 99%

Optogenetic tools for public goods control in Saccharomyces cerevisiae

Nm¹,

Cj³

et al. 2021

Preprint

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Microorganisms live in dense and diverse communities, with interactions between cells guiding community development and phenotype. The ability to perturb specific intercellular interactions in space and time provides a powerful route to determining the critical interactions and design rules for microbial communities. Approaches using optogenetic tools to modulate these interactions offer promise, as light can be exquisitely controlled in space and time. We report new plasmids for rapid integration of an optogenetic system into Saccharomyces cerevisiae to engineer light-control of expression of a gene of interest. In a proof-of-principle study, we demonstrate the ability to control a model cooperative interaction, namely the expression of the enzyme invertase (SUC2) which allows S. cerevisiae to hydrolyze sucrose and utilize it as a carbon source. We demonstrate that the strength of this cooperative interaction can be tuned in space and time by modulating light intensity and through spatial control of illumination. Spatial control of light allows cooperators and cheaters to be spatially segregated, and we show that the interplay between cooperative and inhibitory interactions in space can lead to pattern formation. Our strategy can be applied to achieve spatiotemporal control of expression of a gene of interest in Saccharomyces cerevisiae to perturb both intercellular and interspecies interactions.

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“…The application of cocultures in biotechnological processes is still scarce, as their dynamic control remains challenging at the bulk level. Recently, control mechanisms for cocultures including the engineering of strains for coculture self-regulation via quorum sensing (QS), , toxin-antitoxin interaction, gene transfer, and metabolite exchange have been successfully demonstrated (Table A). Online control of individual coculture members (Table B) or a single coculture member (Table C) has been achieved by the addition of QS molecules or antibiotics .…”

mentioning

confidence: 99%

(Optochemical) Control of Synthetic Microbial Coculture Interactions on a Microcolony Level

et al. 2021

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Synthetic microbial cocultures carry enormous potential for applied biotechnology and are increasingly the subject of fundamental research. So far, most cocultures have been designed and characterized based on bulk cultivations without considering the potentially highly heterogeneous and diverse single-cell behavior. However, an in-depth understanding of cocultures including their interacting single cells is indispensable for the development of novel cultivation approaches and control of cocultures. We present the development, validation, and experimental characterization of an optochemically controllable bacterial coculture on a microcolony level consisting of two Corynebacterium glutamicum strains. Our coculture combines an L-lysine auxotrophic strain together with a L-lysine-producing variant carrying the genetically IPTG-mediated induction of L-lysine production. We implemented two control approaches utilizing IPTG as inducer molecule. First, unmodified IPTG was supplemented to the culture enabling a medium-based control of the production of L-lysine, which serves as the main interacting component. Second, optochemical control was successfully performed by utilizing photocaged IPTG activated by appropriate illumination. Both control strategies were validated studying cellular growth on a microcolony level. The novel microfluidic single-cell cultivation strategies applied in this work can serve as a blueprint to validate cellular control strategies of synthetic mono-and cocultures with single-cell resolution at defined environmental conditions.

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Evaluation of positive interaction for cell growth between Bifidobacterium adolescentis and Propionibacterium freudenreichii using a co-cultivation system with two microfiltration modules

Cited by 8 publications

References 13 publications

Optogenetic Tools for Control of Public Goods in Saccharomyces cerevisiae

Optogenetic Tools for Control of Public Goods in Saccharomyces cerevisiae

Optogenetic tools for public goods control in Saccharomyces cerevisiae

(Optochemical) Control of Synthetic Microbial Coculture Interactions on a Microcolony Level

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