Evaluation of positive ductal margins of biliary tract cancer in intraoperative histological examination

Abstract

At present the only method available to confirm microscopic infiltration of cancer into ductal margins during surgery, is intraoperative histological examination. In the present study, the status of the surgical margins and postoperative course were evaluated to determine any correlation between remnant carcinoma and postoperative survival. All consecutive patients who underwent resection for biliary tract cancer between January 2004 and May 2012 were identified from a database. Positive margin cases were divided into two groups, invasive carcinoma and carcinoma in situ (CIS). Immunohistochemical staining targeting Ki67 and p53 for positive margins was performed. Cases of major vessel invasion were significantly increased in the positive group compared with the negative group. The recurrence rate was significantly lower in the CIS group compared with the invasive group. The survival rate was significantly increased in the CIS group compared with the invasive group. The expression levels of p53 and Ki67 were significantly increased in the invasive group compared with the CIS group. No statistical correlations were observed between the expression of p53 or Ki67 and the survival or recurrence of disease. In the positive group, resected margin status was the principal factor associated with recurrence-free survival according to Cox-regression analysis. In conclusion, the status of the resected margins in the positive group was the most important factor for postoperative survival and recurrence in cholangiocarcinoma, not immunohistochemical staining targeting Ki67 and p53.

Does high-grade dysplasia/carcinoma in situ of the biliary duct margin affect the prognosis of extrahepatic cholangiocarcinoma? A meta-analysis

“…Seventeen records were excluded for duplication by NoteExpress 3.1, and then 345 records were excluded after browsing titles and abstracts. Finally, 22 records were excluded after full-text review for the following reasons: (i) 18 records for unclear grouping; (ii) two records for overlapped cohorts [27, 28]; (iii) one record for mixed gallbladder carcinoma [29]; (iv) one record for review [16]. Hence, 11 records were enrolled into our meta-analysis [12, 17–19, 30–36].…”

The Potential Role of Adjuvant Chemoradiotherapy in Patients with Microscopically Positive (R1) Surgical Margins after Resection of Cholangiocarcinoma

“…Surgical resection is the only potentially curative treatment for biliary tract cancer but, even after radical surgery, recurrence occurs in more than half of patients [13] and negatively impacts long-term outcome. Together with the presence of lymph node involvement, the surgical margin status is the main prognostic factor in resected patients [6][7][8][14][15][16][17]. Prior to 2017, adjuvant therapy in BTC was supported by a large meta-analysis showing a significant benefit in patients with lymph node involvement (OR, 0.49; p = 0.004) and microscopic positive resection margins (R1) (OR, 0.36; p = 0.002) [11].…”

Current Status of Laparoscopic Hepatectomy

“…However, some advantages of LH have been reported for intrahepatic cholangiocarcinoma, namely shorter hospital stays, less intraoperative blood loss, and fewer postoperative complications. For cholangiocarcinoma, the status of the resected margins is the most important factor for postoperative survival 68 . The 3-and 5-year survival rates for LH were similar to those for OH 16 .…”