Evaluation of polymerase chain reaction for detection of Mycoplasma meleagridis infection in turkeys

Moalic, Pierre-Yves; Gesbert, F.; Laigret, Frédéric; Kempf, Isabelle

doi:10.1016/s0378-1135(97)00177-6

Cited by 11 publications

(3 citation statements)

References 20 publications

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“…As underlined by Altwegg (1995), while culture recognizes viable organisms only, amplification tests are not dependant on viable or structurally intact cells and the presence of DNA is sufficient to yield a positive result. Thus the potential for detecting non-viable mycoplasmas explains the discrepancies between PCR and culture results following antibiotic therapy (Kempf et al 1994b;Moalic et al, 1997b) and raise the problem of interpretation of PCR positive culture-negative results.…”

Section: Principal Problems and Aspects Related To Amplification Methodsmentioning

confidence: 99%

DNA amplification methods for diagnosis and epidemiological investigations of avian mycoplasmosis

Kempf¹

1998

Avian Pathology

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This review describes some applications of DNA amplification methods for diagnosis or epidemiological investigations of avian mycoplasmosis. Tests for direct detection of pathogenic mycoplasmas have been developed. Moreover, most avian mycoplasma species can be differentiated, according to their unique restriction fragment length polymorphism (RFLP) patterns generated after digestion of PCR products with different restriction enzymes. In order to characterize isolates below the species level, PCR-based subtyping methods have been introduced. One of them, arbitrarily primed-PCR, results in strain-specific arrays of DNA fragments that can distinguish even closely related strains of a given species. This method was successfully used to investigate the molecular epidemiology of vaccine strains and of Mycoplasma gallisepticum conjunctivitis in songbirds. Major issues in the development of DNA-amplification tests concern the selection of the appropriate target, specimen collection, DNA preparation and detection of amplification reaction inhibitors. Careful consideration to the design and work flow of the facility are necessary to avoid false-positive results.

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Section: Principal Problems and Aspects Related To Amplification Methodsmentioning

confidence: 99%

DNA amplification methods for diagnosis and epidemiological investigations of avian mycoplasmosis

Kempf¹

1998

Avian Pathology

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“…In mature turkeys, however, the organism is localized in the genital tract (Yamamoto, 1991). Recently, an increasing amount of attention has been paid to the trachea (Moalic et al, 1997(Moalic et al, , 1998 because using tracheal swabs on turkeys with respiratory signs permits the isolation of M. meleagridis and the amplification of M. meleagridis DNA sequences by polymerase chain reactions. This recent trend indicates the increasing importance of the upper respiratory tract in the transmission of M. meleagridis.…”

Section: Introductionmentioning

confidence: 99%

Infection of the turkey embryonic trachea withMycoplasma meleagridis

Lam

DaMassa²,

Ghazikhanian³

2003

Avian Pathology

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Mycoplasma meleagridis was used to infect turkey embryos and tracheal explants. In the embryonic trachea, there was a decrease in the number of cilia and the sloughing of epithelial cells. In the tracheal explants, the deciliation was more severe and erosion of the tracheal surface was also evident; additionally, the cells of the trachea showed prominent perforation. These results indicate that M. meleagridis infection can result in damage to cells and to the surface of the trachea, which may have pathological consequences.

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“…In addition, previous treatment of birds with antibiotics, proper storage of samples, and transport of samples can affect the recovery of viable Mycoplasma [7]. Molecular techniques, such as polymerase chain reaction (PCR), are established and are sensitive and highly specific methods for the detection of Mycoplasma [6,8,9]. Several PCR primers targeting different genes of MG were described previously [10].…”

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confidence: 99%

Detection and molecular characterization of Mycoplasma gallisepticum field infection in TS-11-vaccinated broiler breeders

Abdelwhab

Abdelmagid²,

El-Sheibeny³

et al. 2011

Journal of Applied Poultry Research

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Evaluation of polymerase chain reaction for detection of Mycoplasma meleagridis infection in turkeys

Cited by 11 publications

References 20 publications

DNA amplification methods for diagnosis and epidemiological investigations of avian mycoplasmosis

DNA amplification methods for diagnosis and epidemiological investigations of avian mycoplasmosis

Infection of the turkey embryonic trachea withMycoplasma meleagridis

Detection and molecular characterization of Mycoplasma gallisepticum field infection in TS-11-vaccinated broiler breeders

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