Evaluation of polymer and self-assembled monolayer-coated silicone surfaces to reduce neural cell growth

Patel, Kruti R.; Tang, Haiying; Grever, William E.; Ng, Koon‐Kwan; Xiang, Jianming; Keep, Richard F.; Cao, Ting; McAllister, James P.

doi:10.1016/j.biomaterials.2005.08.009

Cited by 34 publications

(37 citation statements)

References 23 publications

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“…Among these hydrophilic substrates, both heparin and hyaluronan contain large functional groups compared to mica, which may account for the long-range attractive force observed in these two surfaces. In parallel studies of astrocyte, choroids plexus (Patel et al, 2006), and Staphylococcus epidermidis (Tang et al, 2005) on the same series of modified silicone surfaces, we also found that FAS was the most effective in reducing adhesion, while heparin enhanced adhesion, which is consistent with the AFM force measurements.…”

Section: Discussionsupporting

confidence: 83%

Nanoscale investigation on adhesion of E. coli to surface modified silicone using atomic force microscopy

Cao

Tang

Liang

et al. 2006

Biotech & Bioengineering

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Bacterial adhesion on biomaterial surfaces is the initial step in establishing infections and leads to the formation of biofilms. In this study, silicone was modified with different biopolymers and silanes, including: heparin, hyaluronan, and self-assembled octadecyltrichlorosilane (OTS), and fluoroalkylsilane (FAS). The aim was to provide a stable and bacteria-resistant surface by varying the degree of hydrophobicity and the surface structure. The adhesion of Escherichia coli (JM 109) on different modified silicone surfaces was investigated by atomic force microscopy (AFM) and scanning electron microscopy (SEM). Mica, an ideal hydrophilic and smooth surface, was employed as a control specimen to study the effect of hydrophobicity and surfaces roughness on bacterial adhesion. AFM probes were coated with E. coli and the force measurements between the bacteria-immobilized tip and various materials surfaces were obtained while approaching to and retracting from the surfaces. A short-range repulsive force was observed between the FAS coated silicone and bacteria. The pull-off force of bacteria to FAS was the smallest among coated surfaces. On the other hand, heparin exhibited a long-range attractive force during approach and required a higher pull-off force in retraction. Both AFM and SEM results indicated that FAS reduced bacterial adhesion whereas heparin enhanced the adhesion compared to pure silicone. The work demonstrates that hydrophobicity cannot be used as a criterion to predict bacterial adhesion. Rather, both the native properties of the individual strain of bacteria and the specific functional structure of the surfaces determine the strength of force interaction, and thus the extent of adhesion.

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Section: Discussionsupporting

confidence: 83%

Nanoscale investigation on adhesion of E. coli to surface modified silicone using atomic force microscopy

Cao

Tang

Liang

et al. 2006

Biotech & Bioengineering

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“…8 In this work, the hydrophobicity of the PLL glass, nanofibrillar scaffolds, Aclar, and PLL Aclar surfaces were characterized by contact angle analysis ( Figure 1). …”

Section: Contact Angle Resultsmentioning

confidence: 99%

“…Previous investigations suggested that surface polarity might be an influential parameter of neural cell responses. 7 Because the macroscopic surface roughness introduced by the nanofibrillar scaffolds differed significantly from previous investigations, 8,9 this parameter was also considered. Additionally, new nanoscopic investigations of surface roughness were performed.…”

Section: Introductionmentioning

confidence: 99%

Nanofibrillar scaffolds induce preferential activation of Rho GTPases in cerebral cortical astrocytes

Tiryaki¹,

Ayres²,

Khan³

et al. 2012

IJN

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Cerebral cortical astrocyte responses to polyamide nanofibrillar scaffolds versus poly-L-lysine (PLL)-functionalized planar glass, unfunctionalized planar Aclar coverslips, and PLL-functionalized planar Aclar surfaces were investigated by atomic force microscopy and immunocytochemistry. The physical properties of the cell culture environments were evaluated using contact angle and surface roughness measurements and compared. Astrocyte morphological responses, including filopodia, lamellipodia, and stress fiber formation, and stellation were imaged using atomic force microscopy and phalloidin staining for F-actin. Activation of the corresponding Rho GTPase regulators was investigated using immunolabeling with Cdc42, Rac1, and RhoA. Astrocytes cultured on the nanofibrillar scaffolds showed a unique response that included stellation, cell-cell interactions by stellate processes, and evidence of depression of RhoA. The results support the hypothesis that the extracellular environment can trigger preferential activation of members of the Rho GTPase family, with demonstrable morphological consequences for cerebral cortical astrocytes.

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“…9 For example, Patel et al showed that silane moieties have an adverse effect on neuronal attachment. 10 In the case of neurons growing on diamond, typical hydrogen-or oxygen-based functionalities have been found to be of little difference to cell proliferation, 7,11,12 except for flat homoepitaxial diamond (as opposed to nanocrystalline diamond, NCD), where hydrophilic oxygenated diamond has been found to be more conducive to neuronal attachment. 11 Chen et al 13 found that neural stem cells grown on hydrogen-terminated ultra-nanocrystalline diamond (UNCD) spontaneously differentiated into neurons, while the cells grown on oxygen-terminated UNCD films exhibited a preference towards oligodendrocyte (insulating glia cells) differentiation.…”

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confidence: 99%

Neuron Growth on Nanodiamond

Edgington

Jackman

2014

Nanodiamond

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Neurons are one of the most environmentally demanding cell types to grow on artificial substrates. As a result, neuronal biomaterials must meet a large list of specifications in order to provide a nurturing environment for neuronal cultures. Such specifications are set out as to mimic the host environment of neurons and, in this context, nanodiamond monolayers are excellent substrates for neuronal culture. Their tissue-equivalent bulk constitution of carbon, their organic surface functionality yet inert chemistry and their nanoscale topography all contribute to nanodiamond's remarkable ability to support the formation of functional neuronal networks, even without the otherwise pre-requisite process of protein coating substrates prior to neuronal seeding. In this chapter we discuss the remarkable ability of nanodiamond monolayers to support the formation of functional neuronal networks on a variety of surfaces. Thereafter, the mechanism of neuronal adhesion on nanodiamond is discussed with respect to the surface properties of different nanodiamond coating types. Finally, nanodiamond-directed patterning of neuronal networks is demonstrated. Nanodiamond coatings provide an excellent growth substrate on various materials for functional, patterned neuronal networks and bypass the necessity of protein coating substrates, which promises great potential for chronic medical implants and in vitro devices.

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Evaluation of polymer and self-assembled monolayer-coated silicone surfaces to reduce neural cell growth

Cited by 34 publications

References 23 publications

Nanoscale investigation on adhesion of E. coli to surface modified silicone using atomic force microscopy

Nanoscale investigation on adhesion of E. coli to surface modified silicone using atomic force microscopy

Nanofibrillar scaffolds induce preferential activation of Rho GTPases in cerebral cortical astrocytes

Neuron Growth on Nanodiamond

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