Evaluation of Polycaprolactone-Associated Human Nasal Chondrocytes as a Therapeutic Agent for Cartilage Repair

Kim, Do Hyun; Lim, Mi Hyun; Jeun, Jung Ho; Park, Sun Hwa; Lee, WeonSun; Park, Sang Hi; Kwon, Mi Yeon; Hwang, Se Hwan; Kim, Sung Won

doi:10.1007/s13770-019-00210-1

Cited by 7 publications

(4 citation statements)

References 37 publications

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“…4,5 It is biocompatible and degrades slowly when implanted in tissue, 4 and therefore has been widely used as both a drug delivery device and bone scaffold in reconstruction therapy. [6][7][8][9] In dentistry, clinical research using PCL evaluated ridge preservation after extraction, 10 and bone graft procedures at the periodontal defect. 11 PCL can also be easily fabricated by three-dimensional (3D) printing technology, allowing for precise customization to various defect configurations, as well as mass production of a product with homogenous mechanical properties that are similar to those of bone.…”

Section: Introductionmentioning

confidence: 99%

In vivo evaluation of 3D printed polycaprolactone composite scaffold and recombinant human bone morphogenetic protein‐2 for vertical bone augmentation with simultaneous implant placement on rabbit calvaria

Chang¹,

Lee

Jeong

et al. 2021

J Biomed Mater Res

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This study evaluated 3D printed polycaprolactone (PCL) composite scaffold and recombinant human bone morphogenetic protein-2 (rhBMP-2), loaded either onto a PCL composite scaffold or implant surface, for vertical bone augmentation with implant placement. Three-dimensional printed PCL frames were filled with powdered PCL, hydroxyapatite, and β-tricalcium phosphate. RhBMP-2 was loaded to the PCL composite scaffolds and implant surfaces, and rhBMP-2 release was quantified for 21 days. Experimental implants were placed bilaterally on 20 rabbit calvaria, and the PCL composite scaffolds were vertically augmented. The randomly allocated experimental groups were divided by carrier and rhBMP-2 dosage as no rhBMP-2 (control), 5 μg rhBMP-2 loaded to PCL composite (Scaffold/rhBMP-2[5 μg]), 5 μg rhBMP-2 loaded to implant (Implant/rhBMP-2[5 μg]), 30 μg rhBMP-2 loaded to PCL composite (Scaffold/rhBMP-2[30 μg]), and 30 μg rhBMP-2 loaded to implant (Implant/rhBMP-2 [30 μg]). Histologic and histometric analyses were conducted after 8 weeks. In both scaffold-loading and implant-loading, rhBMP-2 released initially rapidly, then slowly and constantly. Released rhBMP-2 totaled 23.02 ± 1.03% and 24.69 ± 1.14% in the scaffold-loaded and implant-loaded groups, respectively. There were no significant differences in histologic bone-implant contact (%). Peri-implant bone density (%) was significantly higher in the Scaffold/rhBMP-2(30 μg) and Implant/rhBMP-2(30 μg) groups. Total bone density (%) was not significantly different between the Scaffold/ rhBMP-2(5 μg), Implant/rhBMP-2(5 μg), and control groups, or between the Scaffold/rhBMP-2(30 μg) and Implant/rhBMP-2(30 μg) groups, but was significantly higher in the Scaffold/rhBMP-2(30 μg) and Implant/rhBMP-2(30 μg) groups than in the controls. Three-dimensional printed PCL composite scaffold with rhBMP-2 produced vertical osteogenesis and osseointegration, regardless of rhBMP-2 loading to the PCL composite scaffold or implant surface.Yun-Young Chang and Sa-Ya Lee contributed equally to this study.

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Section: Introductionmentioning

confidence: 99%

In vivo evaluation of 3D printed polycaprolactone composite scaffold and recombinant human bone morphogenetic protein‐2 for vertical bone augmentation with simultaneous implant placement on rabbit calvaria

Chang¹,

Lee

Jeong

et al. 2021

J Biomed Mater Res

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“…29,30 However, MSC multipotency entails the possibility of alternative adipose or osteogenic differentiation. 31 Other research groups have used culture-expanded nasal [32][33][34] or auricular 35 chondrocytes or have compared diverse heterotopic chondrocyte sources. 36 In this study, we aimed to improve rabbit chondrocyteladen, PCL-based scaffolds maturation and test their in vivo performance in a rabbit auricular cartilage model.…”

Section: Introductionmentioning

confidence: 99%

“… 29 , 30 However, MSC multipotency entails the possibility of alternative adipose or osteogenic differentiation. 31 Other research groups have used culture-expanded nasal 32 - 34 or auricular 35 chondrocytes or have compared diverse heterotopic chondrocyte sources. 36 …”

Section: Introductionmentioning

confidence: 99%

Ex Vivo Maturation of 3D-Printed, Chondrocyte-Laden, Polycaprolactone-Based Scaffolds Prior to Transplantation Improves Engineered Cartilage Substitute Properties and Integration

et al. 2022

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Objective The surgical management of nasal septal defects due to perforations, malformations, congenital cartilage absence, traumatic defects, or tumors would benefit from availability of optimally matured septal cartilage substitutes. Here, we aimed to improve in vitro maturation of 3-dimensional (3D)-printed, cell-laden polycaprolactone (PCL)-based scaffolds and test their in vivo performance in a rabbit auricular cartilage model. Design Rabbit auricular chondrocytes were isolated, cultured, and seeded on 3D-printed PCL scaffolds. The scaffolds were cultured for 21 days in vitro under standard culture media and normoxia or in prochondrogenic and hypoxia conditions, respectively. Cell-laden scaffolds (as well as acellular controls) were implanted into perichondrium pockets of New Zealand white rabbit ears ( N = 5 per group) and followed up for 12 weeks. At study end point, the tissue-engineered scaffolds were extracted and tested by histological, immunohistochemical, mechanical, and biochemical assays. Results Scaffolds previously matured in vitro under prochondrogenic hypoxic conditions showed superior mechanical properties as well as improved patterns of cartilage matrix deposition, chondrogenic gene expression ( COL1A1, COL2A1, ACAN, SOX9, COL10A1), and proteoglycan production in vivo, compared with scaffolds cultured in standard conditions. Conclusions In vitro maturation of engineered cartilage scaffolds under prochondrogenic conditions that better mimic the in vivo environment may be beneficial to improve functional properties of the engineered grafts. The proposed maturation strategy may also be of use for other tissue-engineered constructs and may ultimately impact survival and integration of the grafts in the damaged tissue microenvironment.

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“…3,4,9 We performed a critical review of the literature on the use of tissue-engineered cartilage for facial reconstructive surgery. Although much of the research has been focused on nasal septal and dorsum reconstruction, 5,[10][11][12][13][14][15][16][17][18][19][20][21][22][23][24][25][26][27][28] this review will focus on engineering cartilage for nasal alar and auricular defects, because these are of greatest interest for dermatologic surgeons. We aim to provide a targeted, yet comprehensive, overview of the literature and discuss the benefits of using tissue-engineered cartilage for reconstruction of alar and auricular defects after Mohs micrographic surgery (MMS).…”

mentioning

confidence: 99%

Cartilage Tissue Engineering for Nasal Alar and Auricular Reconstruction: A Critical Review of the Literature and Implications for Practice in Dermatologic Surgery

Himeles

Ratner

2023

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BACKGROUND Reconstructing defects requiring replacement of nasal or auricular cartilage after Mohs micrographic surgery can at times be challenging. While autologous cartilage grafting is considered the mainstay for repair, it may be limited by cartilage quality/quantity, donor site availability/morbidity, and surgical complications. Tissue-engineered cartilage has recently shown promise for repairing properly selected facial defects. OBJECTIVE To (1) provide a comprehensive overview of the literature on the use of tissue-engineered cartilage for nasal alar and auricular defects, and (2) discuss this technology's advantages and future implications for dermatologic surgery. MATERIALS AND METHODS A literature search was performed using PubMed/MEDLINE and Google Scholar databases. Studies discussing nasal alar or auricular cartilage tissue engineering were included. RESULTS Twenty-seven studies were included. Using minimal donor tissue, tissue-engineered cartilage can create patient-specific, three-dimensional constructs that are biomechanically and histologically similar to human cartilage. The constructs maintain their shape and structural integrity after implantation into animal and human models. CONCLUSION Tissue-engineered cartilage may be able to replace native cartilage in reconstructing nasal alar and auricular defects given its ability to overcome several limitations of autologous cartilage grafting. Although further research is necessary, dermatologic surgeons should be aware of this innovative technique and its future implications.

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Evaluation of Polycaprolactone-Associated Human Nasal Chondrocytes as a Therapeutic Agent for Cartilage Repair

Cited by 7 publications

References 37 publications

In vivo evaluation of 3D printed polycaprolactone composite scaffold and recombinant human bone morphogenetic protein‐2 for vertical bone augmentation with simultaneous implant placement on rabbit calvaria

In vivo evaluation of 3D printed polycaprolactone composite scaffold and recombinant human bone morphogenetic protein‐2 for vertical bone augmentation with simultaneous implant placement on rabbit calvaria

Ex Vivo Maturation of 3D-Printed, Chondrocyte-Laden, Polycaprolactone-Based Scaffolds Prior to Transplantation Improves Engineered Cartilage Substitute Properties and Integration

Cartilage Tissue Engineering for Nasal Alar and Auricular Reconstruction: A Critical Review of the Literature and Implications for Practice in Dermatologic Surgery

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