Evaluation of pollen viability, stigma receptivity, and the cross barrier between tropical and hardy water lily cultivars

Hao, Qing; Xu, Lihui; Wang, Hai; Liu, Qinghua; Wang, Kuiling

doi:10.1016/j.flora.2022.152046

Cited by 4 publications

(12 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Higher IAA, ZR levels and (IAA + ZR) / ABA levels favored pollination while lower ABA levels were detrimental during pollen-stigma recognition, adhesion and germination (Yang et al ., 2022). In the process of hybrid breeding in water lily, the difficulty of pollen germination on the stigma is the main factor leading to inter-subgeneric hybrid barriers (Hao et al ., 2022; Sun et al ., 2023; Sun et al ., 2019; Sun et al ., 2018). In production, the spraying of exogenous hormones can be used to promote and increase the success rate of pollination, ensuring the progress of hybrid seed production (Daza et al ., 2021).…”

Section: Discussionmentioning

confidence: 99%

“…Tropical water lily is favored for their diverse flower colors, including red, blue, purple, white, yellow and other complex hues (Yu et al ., 2018). However, tropical water lily is intolerant to low temperatures and struggle to survive harsh winters in higher latitudes (Hao et al ., 2022). On the other hand, hardy water lily, found in temperate regions, possess unique cold tolerance (Sun et al ., 2023; Sun et al ., 2021).…”

Section: Introductionmentioning

confidence: 99%

“…Therefore, hybrid breeding between tropical and hardy water lily holds great significance in cultivars that are both cold-tolerant and possess diverse flower colors. However, the hybridization between tropical and hardy water lily has proven to be challenging, with low seed set and inefficient pollination (Hao et al ., 2022; Sun et al ., 2019; Sun et al ., 2018). Studies have shown that pre-fertilization hybridization barriers during the cross between tropical and hardy water lily are the main hurdles in achieving successful seed production (Hao et al ., 2022; Sun et al ., 2019; Sun et al ., 2018), with low pollen germination rate being a commonly observed phenomenon.…”

Section: Introductionmentioning

confidence: 99%

“…However, the hybridization between tropical and hardy water lily has proven to be challenging, with low seed set and inefficient pollination (Hao et al ., 2022; Sun et al ., 2019; Sun et al ., 2018). Studies have shown that pre-fertilization hybridization barriers during the cross between tropical and hardy water lily are the main hurdles in achieving successful seed production (Hao et al ., 2022; Sun et al ., 2019; Sun et al ., 2018), with low pollen germination rate being a commonly observed phenomenon. Additionally, abnormalities in pollen tube growth, such as bending or swelling at the tip and failure to penetrate the ovule, have been observed (Hao et al ., 2022).…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Hormonal and transcriptomic analysis reveals the role of ABA and BR in breaking the barrier of inter-subgeneric hybridization in water lily (Nymphaea)

Zhou,

Li,

Jiang

et al. 2023

Preprint

View full text Add to dashboard Cite

Understanding the process of signal communication between pollen and stigma is of significant importance for plant sexual reproduction. In the case of inter-subgeneric hybridization in water lily, there exists a pre-fertilization hybridization barrier, the regulatory mechanism of which remains unclear. In this study, we conducted hormone and transcriptome analyses of unpollinated stigmas (Mock), self-pollinated stigmas (SP), cross-pollinated stigmas within the same subgenus (CP), and inter-subgenus cross-pollination stigmas (ISCP) in water lily to elucidate the formation mechanism of the inter-subgeneric hybridization barrier. Our results indicated that the lack of ABA and BR in ISCP stigmas are key factors contributing to the formation of the inter-subgeneric hybridization barrier in water lily. Exogenous application of ABA and BR can help overcome the barrier between inter-subgeneric water lily crosses. Through transcriptome analysis, we identified nine candidate genes involved in regulating the inter-subgeneric hybridization barrier in water lily. In addition, we further demonstrated the importance of the NCED2-mediated ABA synthesis pathway in the hybridization process through AS-ODN technology. Our study confirms that ABA and BR are critical for breaking the inter-subgeneric hybridization barrier in water lily. The identification of the nine candidate genes provides important clues for further research on the hybridization recognition mechanism in water lily.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Hormonal and transcriptomic analysis reveals the role of ABA and BR in breaking the barrier of inter-subgeneric hybridization in water lily (Nymphaea)

Zhou,

Li,

Jiang

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

“…Proper germination and growth of pollen grains are essential for fertilization, fruit, and seed development (Shivanna and Rangswamy, 2012). in vitro pollen germination is used significantly on a variety of pollen frameworks (Hao et al, 2022). The study of pollen germination is vital in plant developmental biology.…”

Section: Introductionmentioning

confidence: 99%

Pollen storage, viability and effect of growth hormones on in vitro pollen germination in two medicinal plants (Clerodendrum colebrookianum Walp. and Clerodendrum infortunatum L.) of the tropical moist forest of North-east India

Singh

Kumar

Khanduri

2022

JANS

View full text Add to dashboard Cite

Clerodendrum colebrookianum Walp. and Clerodendrum infortunatum L. are well known for their medicinal uses in treating various human ailments traditionally. Pollen biology study was done in both plant species to decipher pollen viability, in vitro pollen germination, and pollen storage in varied temperature conditions. Pollen viability tests was done by 2, 3, 5-triphenyl tetrazolium chloride (TTC test) for which pollen grains were collected at the anthesis stage that ascertained high viability, ranged between 71.97%±4.30 % in C. colebrookianum and 81.63%±3.23 in C. infortunatum. In vitro pollen germination was conducted under different hormones (IBA, IAA, GA3, and Kinetin) with concentrations of 100 mg L-1, 200 mg L-1 and 300 mg L-1. Highest germination percentage of 52.10±5.30% and 61.91±1.76% in GA3 (200 mg L-1) was the most suitable growth hormone concentration for inducing in vitro pollen germination in both Clerodendrum species. Statistically, the response of all the concentrations of treatments, sucrose, and hormones, with their time on in vitro pollen germination of C. colebrookianum and C. infortunatum was found significantly different (p<0.05). In contrast, non-significant differences were recorded for in vitro pollen germination between the medicinal plant species. Pollen storage under temperature gradient conditions exhibited a similar trend in the viability for both C. colebrookianum, and C. infortunatum, i.e., the pollen remained viable up to 28 days at -20°C and 6°C, respectively. This study will be helpful in future breeding, hybridization, and conservation efforts for both the Clerodendrum species.

show abstract

Hormone and transcriptomic analysis revealed that ABA and BR are key factors in the formation of inter‐subgeneric hybridization barrier in water lily

Zhou,

Li,

Jiang

et al. 2024

Physiologia Plantarum

View full text Add to dashboard Cite

Understanding the process of signal communication between pollen and stigma is of significant importance for plant sexual reproduction. In the case of inter‐subgeneric hybridization in water lily, a pre‐fertilization hybridization barrier exists, the regulatory mechanism of which remains unclear. In this study, we conducted hormone and transcriptome analyses of unpollinated stigmas (Mock), self‐pollinated stigmas (SP), cross‐pollinated stigmas within the same subgenus (CP), and inter‐subgenus cross‐pollination stigmas (ISCP) in water lily to elucidate the formation mechanism of the inter‐subgeneric hybridization barrier. Our results indicated that the lack of abscisic acid (ABA) and brassinolide (BR) in ISCP stigmas are key factors contributing to the formation of the inter‐subgeneric hybridization barrier in water lily. Exogenous application of ABA and BR can help overcome the barrier between inter‐subgeneric water lily crosses. Through transcriptome analysis, we identified nine candidate genes involved in regulating the inter‐subgeneric hybridization barrier in water lily. In addition, we further demonstrated the importance of the NCED2‐mediated ABA synthesis pathway in the pollination process through AS‐ODN technology. Our study confirms that ABA and BR are critical for breaking the inter‐subgeneric hybridization barrier. The identification of the nine candidate genes provides important clues for further research on the hybridization recognition mechanism in water lily.

show abstract

Evaluation of pollen viability, stigma receptivity, and the cross barrier between tropical and hardy water lily cultivars

Cited by 4 publications

References 40 publications

Hormonal and transcriptomic analysis reveals the role of ABA and BR in breaking the barrier of inter-subgeneric hybridization in water lily (Nymphaea)

Hormonal and transcriptomic analysis reveals the role of ABA and BR in breaking the barrier of inter-subgeneric hybridization in water lily (Nymphaea)

Pollen storage, viability and effect of growth hormones on in vitro pollen germination in two medicinal plants (Clerodendrum colebrookianum Walp. and Clerodendrum infortunatum L.) of the tropical moist forest of North-east India

Hormone and transcriptomic analysis revealed that ABA and BR are key factors in the formation of inter‐subgeneric hybridization barrier in water lily

Contact Info

Product

Resources

About