Evaluation of Pollen Viability by Enzymatically Induced Fluorescence; Intracellular Hydrolysis of Fluorescein Diacetate

Heslop‐Harrison, J.; Heslop‐Harrison, Y.

doi:10.3109/10520297009085351

Cited by 785 publications

(336 citation statements)

References 4 publications

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“…3b) implies that the integrity of the plasmalemma of the vegetative cell is preserved. This way, FDA test provides a more effective method for assessing pollen quality (Heslop-Harrison and Heslop-Harrison, 1970). As expected, the frequencies of viable pollen grains in the present study were higher with propionic-carmine staining when compared to FDA.…”

Section: Developmental Stagesupporting

confidence: 68%

Initial segmentation patterns of microspores and pollen viability in soybean cultured anthers: indication of chromosome doubling

Cardoso

Kaltchuk-Santos

Mundstock

et al. 2004

Braz. arch. biol. technol.

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Anthers obtained from flowers buds of soybean cultivar IAS -5 were cultured in two basal culture media (B5 and B5 long). Cytological examinations of the in vitro anthers were performed during the first 20 days of culture to assay the viability (by propionic-carmine and fluorescein diacetate tests) and the stage of development of pollen grains. The frequencies of viable pollen grains varied significantly between bud sizes on the propionic-carmine analysis.The basal culture media and bud size had no clear effect on the frequencies of binucleate symmetrical and multinucleate pollen grains. Chromosome counts of metaphasic microspores throughout the culture period showed microspores with higher ploidy level in addition to normal chromosome number (n=20).

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Section: Developmental Stagesupporting

confidence: 68%

Initial segmentation patterns of microspores and pollen viability in soybean cultured anthers: indication of chromosome doubling

Cardoso

Kaltchuk-Santos

Mundstock

et al. 2004

Braz. arch. biol. technol.

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“…The structural integrity of the coatless pollen was compared with intact control pollen by the fluorescein acetate staining method of Heslop-Harrison and Heslop-Harrison (1970). In both cases, more than 95% of the pollen grains were intact.…”

Section: Pollen Viabilitymentioning

confidence: 99%

Characterization of anther‐expressed genes encoding a major class of extracellular oleosin‐like proteins in the pollen coat of Brassicaceae^†

Ross

Murphy²

1996

The Plant Journal

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SummaryA large, heterogeneous, highly expressed gene family encoding oleosin-like proteins is described in the Brassicaceae. Seven related cDNA sequences were isolated from Brassica napus anther mRNA using RACE-PCR and compared with other recently described anther-specific oleosin-like genes from B. napus. The expression patterns of four representative members of this diverse gene family were analyzed by Northern blotting and in situ hybridization. In all cases, the genes were expressed specifically in the tapatum of 3-5 mm B. napus buds, which contained microspores at the late-vacuolate and bicellular stages of development. The predicted protein products are ordered into subclasses, each of which has a characteristic C-terminal domain, containing different amino acid motifs or repeated residues. Tryphine (pollen coat) fractions from mature B. napus pollen were found to be particularly enriched in polypeptides of apparent molecular weights 32-38 kDa, plus numerous less abundant polypeptides of less than 15 kDa, The N-terminal 15-20 residues of three of these polypeptides (12, 32 and 38 kDa) were found by microsequencing to be identical to parts of the predicted amino acid sequences of three of the tapetal-expressed oleosin-like genes. This indicates the possibility of posttranslational modification of these proteins resulting in a cleavage of the primary translation products in order to generate the mature tryphine polypeptides. These data implythat a large and diverse group of oleosin-like proteins is synthesized in the tapatum of B. napus anthers and that

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“…The general morphology of ms35 homozygotes (leaf shape, mean height, inflorescence and floral morphology) was indistinguishable from that of ms35\j heterozygotes and the Ler wild type (not shown). Viability of ms35 pollen was demonstrated by its deep purple coloration following staining by the procedure of Alexander (1969), by its ability to hydrolyse fluorescein diacetate (Heslop-Harrison & Heslop-Harrison, 1970), and as previously shown (Dawson et al, 1993), by an in vitro germination test (Pickert, 1988). Furthermore, self-pollination of ms35 could be achieved by gently macerating anthers taken from a newly opened flower and spreading the macerated material onto a stigma.…”

Section: Characterization Of the Male Sterile Mutant Ms35mentioning

confidence: 99%

Characterization and genetic mapping of a mutation (ms35) which prevents anther dehiscence in Arabidopsis thaliana by affecting secondary wall thickening in the endothecium

et al. 1999

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The male sterile mutant, ms35, of Arabidopsis thaliana was produced by X-irradiation of seeds. The mutant produces fertile pollen, but is male sterile because the anthers do not dehisce. Anther development in ms35 plants occurs as in wild-type Arabidopsis until shortly after microspores are released from meiotic tetrads. Thereafter, in the wild type, bands of lignified, cellulosic secondary wall thickenings are laid down around the cells of the anther endothecium. In contrast, wall thickenings are not formed in the endothecium of the ms35 mutant. Development of other lignified tissues, for example the vascular tissue of the stamen, occurs normally in ms35 plants. In mutant anthers, as pollen maturation is completed, the stomium is cleaved but the anther wall does not retract to release pollen. The block in anther dehiscence in ms35 plants is specifically correlated with the absence of endothecial wall thickenings. The ms35 mutation represents the first genetic evidence in support of the proposed role of the endothecium in anther dehiscence. The ms35 gene was mapped to the top arm of chromosome 3 (hy2-(4.17p 2.31 cM)-ms35-(32.14p5.45 cM)-gl1).

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Evaluation of Pollen Viability by Enzymatically Induced Fluorescence; Intracellular Hydrolysis of Fluorescein Diacetate

Cited by 785 publications

References 4 publications

Initial segmentation patterns of microspores and pollen viability in soybean cultured anthers: indication of chromosome doubling

Initial segmentation patterns of microspores and pollen viability in soybean cultured anthers: indication of chromosome doubling

Characterization of anther‐expressed genes encoding a major class of extracellular oleosin‐like proteins in the pollen coat of Brassicaceae^†

Characterization and genetic mapping of a mutation (ms35) which prevents anther dehiscence in Arabidopsis thaliana by affecting secondary wall thickening in the endothecium

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Evaluation of Pollen Viability by Enzymatically Induced Fluorescence; Intracellular Hydrolysis of Fluorescein Diacetate

Cited by 785 publications

References 4 publications

Initial segmentation patterns of microspores and pollen viability in soybean cultured anthers: indication of chromosome doubling

Initial segmentation patterns of microspores and pollen viability in soybean cultured anthers: indication of chromosome doubling

Characterization of anther‐expressed genes encoding a major class of extracellular oleosin‐like proteins in the pollen coat of Brassicaceae†

Characterization and genetic mapping of a mutation (ms35) which prevents anther dehiscence in Arabidopsis thaliana by affecting secondary wall thickening in the endothecium

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Characterization of anther‐expressed genes encoding a major class of extracellular oleosin‐like proteins in the pollen coat of Brassicaceae^†