Evaluation of Pollen-Mediated Gene Flow from Purple-Colored Maize Line during Co-Cultivation with Yellow-Grain Maize Lines

Gusev, Yu. S.; Volokhina, I. V.; Moiseeva, E. M.; Gutorova, O. V.; Chumakov, M. I.

doi:10.1134/s1022795420090082

Cited by 2 publications

(1 citation statement)

References 8 publications

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“…PMGF has been well documented from GE to non‐GE crops and closely related species (Yook et al., 2021) and is a concern for non‐GE producers who wish for their crops to be free from genetic contamination. Several researchers have reported considerable PMGF from GE corn to white corn (Baltazar et al., 2015), field corn (Bøhn et al., 2016; Gusev et al., 2020), and popcorn ( Zea mays L. var. everta ) (Barnes et al., 2020).…”

Section: Introductionmentioning

confidence: 99%

Pollen‐mediated gene flow from herbicide‐resistant yellow corn to non‐genetically engineered food‐grade white corn

Singh,

Kumar,

Knezevic

et al. 2024

Crop Science

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Nebraska is the number one producer of food‐grade white corn in the United States. Pollen‐mediated gene flow (PMGF) from genetically engineered high alpha‐amylase corn, known as Enogen corn, to food‐grade white corn can have undesirable outcomes. Alpha‐amylase can convert starch in white corn to sugar during or after its processing, degrading the quality of processed products. Thus, proximity to Enogen corn puts white corn production at risk. The objectives of this study were to evaluate the PMGF from herbicide‐resistant yellow corn to food‐grade white corn and assess the significance of wind speed and direction. Field experiments were set up using a Nelder‐wheel design in 2021 and 2022 in Nebraska, with yellow field corn as pollen donor in the center and white corn surrounding it as pollen receptor. At the end of the season, samples of white corn cobs were collected up to 50 and 70 m from four cardinal and ordinal directions, respectively. PMGF was detected by counting the number of yellow kernels on the white cobs. More than 4 million kernels were screened and the highest frequency of PMGF (0.0621–0.1950) was detected at the nearest distance (1 m). PMGF decreased exponentially with distance; however, it was still observed (0.0020–0.0032) at the greatest distance (70 m) evaluated in this study. Wind profile played a significant role in PMGF. Wind frequency (r = 0.58 ≤ 0.86) and wind run (r = 0.36 ≤ 0.95) were moderately to strongly correlated with PMGF in 2021 and 2022, respectively. The results are concerning for white corn growers due to the coexistence of Enogen corn and food‐grade white corn.

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Section: Introductionmentioning

confidence: 99%

Pollen‐mediated gene flow from herbicide‐resistant yellow corn to non‐genetically engineered food‐grade white corn

Singh,

Kumar,

Knezevic

et al. 2024

Crop Science

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High maize barrier prevents maize pollen transfer in mixed crops

Chumakov

Gutorova

Gusev

2021

Ecological genetics

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BACKGROUND: There is no scientifically-based assessment for the safety during co-cultivation of genetically modified and ordinary maize plants in Russia. The influence of the barrier from high maize plants for pollen-mediated gene flow in the 2020 field experiment (South-East of the European part of Russia, Saratov region) was evaluated. MATERIALS AND METHODS: We used the high (2,152,90 m) maize hybrids (Kaz LK 178 and ES Regain) as a barrier for pollen-madiated gene flow from pollen donor (Purple Saratovskaya) with purple grain to recipient (Bursting Corn) maize line with yellow grain. RESULTS: The analysis of the ears of the recipient maize line showed that not a single purple grain was found on them. It was found that in the presence of a barrier zone with a width of 315 m (depending on the direction), crossing in recipient maize line in all directions from the donor is completely excluded. CONCLUSIONS: It was established, for the first time, that the barrier from high maize hybrid plants completely excludes over-pollination between donor and Bursting Corn, recipient maize plants with different flowering time. In the study of barrier plants as pollen recipients, it was found that the percentage of crosses on the cob of barrier plants ranged from 0.1 to 7.1%. The number of crosses exceeding 0.9% is observed mainly in the ES Regain variety at close (15 m) distances from the donor, regardless of the prevailing wind direction. Based on the results of model experiments, it can be recommended to use a barrier for corn pollen from tall maize plants and maize varieties with different flowering periods to exclude uncontrolled over-pollination of maize varieties in the South-East of the European part of Russia conditions.

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Evaluation of Pollen-Mediated Gene Flow from Purple-Colored Maize Line during Co-Cultivation with Yellow-Grain Maize Lines

Cited by 2 publications

References 8 publications

Pollen‐mediated gene flow from herbicide‐resistant yellow corn to non‐genetically engineered food‐grade white corn

Pollen‐mediated gene flow from herbicide‐resistant yellow corn to non‐genetically engineered food‐grade white corn

High maize barrier prevents maize pollen transfer in mixed crops

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