Evaluation of platelet function and of serum fibrinogen levels in patients bitten by snakes of the genus Crotalus: preliminary report

Thomazini, I. A.; Iuan, F. C.; Carvalho, Isabela Feitosa de; Hernandes, Denise H.; Amaral, Ivanete F.; Pereira, Paulo Câmara Marques; Viera, Benedito Barra

doi:10.1590/s0036-46651991000300009

Cited by 8 publications

(5 citation statements)

References 7 publications

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“…Thomazini et al (24) analyzed serum fibrinogen of patients bitten by Crotalus snakes and reported a reduction in the values before antivenom treatment that tended to normalize after 48 hours. These results differ from those found in our experiment, where there was a significant increase in both groups in the three weeks after the first inoculation and decreased in the following weeks.…”

Section: Resultsmentioning

confidence: 99%

“…Hematological changes in erythrocytes, leukocytes, platelets, coagulation factors (3), and fibrinogen (1,24) in rats with a mixture of venoms from different Brazilian snakes, there was a slow and gradual decrease hematocrit values, erythrocyte and lymphocyte counts, and an increase in number of neutrophils and hemoglobin concentration. The number of total leukocytes oscillated, with decreases and increases throughout the experimental period (3).…”

Section: Introductionmentioning

confidence: 99%

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Hematological changes in sheep inoculated with natural and Cobalt60-irradiated Crotalus durissus terrificus venom (Laurenti, 1768)

Netto¹,

Chiacchio²,

Bicudo³

et al. 2004

J. Venom. Anim. Toxins incl. Trop. Dis

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ABSTRACT. Natural (NV) and Cobalto 60 -irradiated (IrV) Crotalus durissus terrificus venom were used to evaluate serum production capacity of sheep and possible hematological and biochemical effects. Freeze-dried venom aliquots were diluted in acidified saline solution (NaCl 150 mM, pH 3.0) and irradiated by a Cobalt 60 source at a dose of 5.54 x 102 Gy/h and a concentration of 2.000 Gy. Twelve sheep were divided into two groups of six animals. One group received irradiated venom (IrV) and the other natural venom (NV). Three antigen doses (venom) were administered at monthly intervals. Blood samples were collected weekly for analysis of serum neutralization potency and capacity, complete blood count (CBC), total plasma protein, fibrinogen, albumin, and globulin. At the end of the experiment, the animals were challenged with a LD 50 for sheep and showed no signs of envenoming. The two groups did not present clinical alterations. Results of the total leukocyte count did not present interaction or time factor effect for both groups, but there was a different action between them, with the NV group presenting more cells than the IrV group. The leukocyte increase to 13,000/µl indicates that slight leukocytosis occurred in the week after the first inoculation in the NV group. There was no statistically significant difference between groups in the absolute count of segmented neutrophils, eosinophils, and lymphocytes but there were statistically significant oscillations in values at the different collecting times. The NV group presented an increase in the absolute neutrophil count after the first inoculation that persisted for 5 weeks. In the IrV group, the increase in neutrophils occurred only in the first week returning to normal in the following weeks. The alterations in the neutrophil count are indicative of systemic inflammatory response related to cytokine release; response was more marked in the NV group, showing its greater toxicity.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Hematological changes in sheep inoculated with natural and Cobalt60-irradiated Crotalus durissus terrificus venom (Laurenti, 1768)

Netto¹,

Chiacchio²,

Bicudo³

et al. 2004

J. Venom. Anim. Toxins incl. Trop. Dis

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“…Crotalus durissus terrificus venom causes hematological alterations in erythrocytes, leukocytes, platelets, coagulation factors (6) and fibrinogen (3,40) when inoculated into humans and animals (6).…”

Section: Introductionmentioning

confidence: 99%

Laboratory evaluation of young ovines inoculated with natural or 60co-irradiated Crotalus durissus terrificus venom during hyperimmunization process

Ferreira

Nascimento

Couto

et al. 2006

J. Venom. Anim. Toxins incl. Trop. Dis

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Laboratory profile of young ovines was studied in order to evaluate and compare their antiserum production from natural and Cobalt-60 irradiated Crotalus durissus terrificus (C.d.t.) venoms. The parameters analyzed included complete blood count, and urea, creatinine, aspartate aminotransferase, total proteins, albumin and globulin serum measurements. Three groups of six animals each were used. Group 1 (G1) received natural C.d.t. venom; Group 2 (G2) received irradiated C.d.t. venom; and Group 3 (G3) was used as control and did not receive venom, only adjuvants, using seven venom inoculations. During the experimental period, animals were fortnightly weighed. According to clinical and weight evaluation, sheep in post-weaning phase showed no changes in their physiological profiles but had excellent weight gain. The parameters analyzed were not statistically different (p<5%) among the groups tested. The hyperimmunization process was successfully accomplished with the production of specific antibodies against Crotalus durissus terrificus venom. Results bring a new possibility of utilizing ovines in the commercial production of anticrotalic serum, which may be used to treat human and animal envenomation. Its production cost may be reduced by subsequent use of hyperimmunized sheep for human consumption

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“…Outro componente importante da peçonha é uma enzima similar à trombina, que possui a capacidade de transformar fibrinogênio em fibrina e está implicada nos distúrbios da coagulação presentes em cerca de 40 a 50% dos pacientes com envenenamento crotálico (BARRAVIERA 1990;JORGE, RIBEIRO 1992). Os pacientes podem apresentar sangue incoagulável com afibrinogenemia sem consumo de plaquetas (AMARAL et al 1988;THOMAZINI et al 1991;SANO-MARTINS et al 2001).…”

Section: A Peçonhaunclassified

“…anos, e trabalhadores rurais (BARRAVIERA 1990;JORGE, RIBEIRO 1990, 1992FEITOSA et al 1997 -MARQUES et al 1985-MARQUES et al , 1987-MARQUES et al , 1992MAGALHÃES et al 1986;CUPO et al 1988CUPO et al , 1990BARRAVIERA 1990;JORGE, RIBEIRO 1990 (AMARAL et al 1980(AMARAL et al , 1988, 1992BARRAVIERA 1990;THOMAZINI et al 1991;BRASIL 2001).…”

Section: Quadro Clínicomentioning

confidence: 99%

Insuficiência renal aguda após envenenamento crotálico: prevalência e fatores de risco - um estudo prospectivo

Pinho¹

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Evaluation of platelet function and of serum fibrinogen levels in patients bitten by snakes of the genus Crotalus: preliminary report

Cited by 8 publications

References 7 publications

Hematological changes in sheep inoculated with natural and Cobalt60-irradiated Crotalus durissus terrificus venom (Laurenti, 1768)

Hematological changes in sheep inoculated with natural and Cobalt60-irradiated Crotalus durissus terrificus venom (Laurenti, 1768)

Laboratory evaluation of young ovines inoculated with natural or 60co-irradiated Crotalus durissus terrificus venom during hyperimmunization process

Insuficiência renal aguda após envenenamento crotálico: prevalência e fatores de risco - um estudo prospectivo

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