Evaluation of plant protection efficacy in field conditions and side effects of<i>Lysobacter capsici</i>AZ78, a biocontrol agent of<i>Plasmopara viticola</i>

Markellou, Emilia; Kapaxidi, Eleftheria V.; Karamaouna, Filitsa; Samara, Maria; Kyriakopoulou, Katerina; Anastasiadou, Pelagia; Vavoulidou, Evangelia; Meidanis, Marios; Machera, Kyriaki; Mandoulaki, Athanasia; Margaritopoulou, Theoni; Giovannini, O.; Tomada, Selena; Pertot, I.; Puopolo, Gerardo

doi:10.1080/09583157.2022.2064431

Cited by 8 publications

(7 citation statements)

References 24 publications

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“…Ad planta tests against Pl. viticola on grapevine with the closely related species L. capsici were conducted by [ 21 , 22 ].…”

Section: Discussionmentioning

confidence: 99%

“…Several species of the genus Lysobacter were reported as interesting candidates for biocontrol purposes to suppress plant diseases [ 18 ]. For example, L. capsici showed activity against downy mildew on grapevine, Fusarium oxysporum on tomato plants and the damping-off of sugar beets [ 19 , 20 , 21 , 22 ], L. antibioticus controlled Xanthomonas oryzae on rice [ 23 ], and L. enzymogenes effectively suppressed Fusarium spp. on cereals and Pythium aphanidermatum on cucumbers [ 24 , 25 ].…”

Section: Introductionmentioning

confidence: 99%

“…It was shown that the studied isolate of L. capsici had no negative impacts on the following non-target organisms: Aphidius rhopalosiphi (parasitic wasp), Typhlodromus pyri (predatory mite), Daphnia magna (aquatic crustacean) and Selenastrum carpricornutum (alga) as well as Eisenia fetida and Enchytraeid albidus (earthworms). Application on grapevine against Plasmopara viticola did not impair the processing (fermentation) of grapes or the quality of the resulting wine [ 22 ].…”

Section: Introductionmentioning

confidence: 99%

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Characterization of a Disease-Suppressive Isolate of Lysobacter enzymogenes with Broad Antagonistic Activity against Bacterial, Oomycetal and Fungal Pathogens in Different Crops

Drenker

Mazouar

Bücker

et al. 2023

Plants

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Although synthetic pesticides play a major role in plant protection, their application needs to be reduced because of their negative impact on the environment. This applies also to copper preparations, which are used in organic farming. For this reason, alternatives with less impact on the environment are urgently needed. In this context, we evaluated eight isolates of the genus Lysobacter (mainly Lysobacter enzymogenes) for their activity against plant pathogens. In vitro, the investigated Lysobacter isolates showed broad antagonistic activity against several phytopathogenic fungi, oomycetes and bacteria. Enzyme assays revealed diverse activities for the tested isolates. The most promising L. enzymogenes isolate (LEC) was used for further detailed analyses of its efficacy and effective working concentrations. The experiments included in vitro spore and sporangia germination tests and leaf disc assays as well as ad planta growth chamber trials against Alternaria solani and Phytophthora infestans on tomato plants, Pseudoperonospora cubensis on cucumbers and Venturia inaequalis on young potted apple trees. When applied on leaves, dilutions of a culture suspension of LEC had a concentration-dependent, protective effect against the tested pathogens. In all pathosystems tested, the effective concentrations were in the range of 2.5–5% and similarly efficacious to common plant protection agents containing copper hydroxide, wettable sulphur or fenhexamid. Thus, the isolate of L. enzymogenes identified in this study exhibits a broad activity against common plant pathogens and is therefore a promising candidate for the development of a microbial biocontrol agent.

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“…Ad planta tests against Pl. viticola on grapevine with the closely related species L. capsici were conducted by [ 21 , 22 ].…”

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Characterization of a Disease-Suppressive Isolate of Lysobacter enzymogenes with Broad Antagonistic Activity against Bacterial, Oomycetal and Fungal Pathogens in Different Crops

Drenker

Mazouar

Bücker

et al. 2023

Plants

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“…Lysobacter capsici AZ 78 is considered to be a good biological control agent because it inhibits the growth of P. viticola and at the same time is resistant to abiotic stress and some concentrations of copper products, having a copper oxidase that reduces the attack of P. viticola as well as effective as copper products (Puoppolo et al, 2014). This biological control agent is as effective as the reference fungicide Kocide© 2000 and does not affect non-target organisms and wine yeasts and could be developed as a commercial biopesticide (Markellou et al, 2022).…”

Section: Discussionmentioning

confidence: 99%

THE USE OF BIOPREPARATIONS WITH BACTERIA AND FUNGI TO CONTROL DOWNY MILDEW (Plasmopara vitícola) AND REDUCE CHEMICAL TREATMENTS

Lixandru¹,

Fendrihan²

2022

Rom. J. Plant. Prot.

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This paper reviews the possible biological control of grapevine downy mildew Plasmopara viticola. Often, when the climatic conditions are very favorable for the emergence and manifestation of the disease, the chemical treatment has a low efficiency, this phytopathogen producing significant damage, including as a result of the formation of resistant strains. A modern method of combating it is biological control using microorganisms bacteria and fungi which can inhibit through their presence directly or indirectly the growth and multiplication of the fungus, which we present in this paper.

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“…The higher abundance of Lysobacter species in the rhizosphere is related to a stronger suppression effect on plant pathogens [ 13 ]. Several species, such as Lysobacter capsici [ 14 ], Lysobacter antibioticus [ 15 , 16 ] and Lysobacter enzymogenes [ 17 ], have been applied to fight against plant fungal pathogens [ 14 , 17 ], bacterial pathogens [ 15 ] and worms [ 16 ]. Lysobacter species utilize various antimicrobial mechanisms.…”

Section: Introductionmentioning

confidence: 99%

Genetic Basis and Expression Pattern Indicate the Biocontrol Potential and Soil Adaption of Lysobacter capsici CK09

Yang

Yuan

et al. 2023

Microorganisms

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Lysobacter species have attracted increasing attention in recent years due to their capacities to produce diverse secondary metabolites against phytopathogens. In this research, we analyzed the genomic and transcriptomic patterns of Lysobacter capsici CK09. Our data showed that L. capsici CK09 harbored various contact-independent biocontrol traits, such as fungal cell wall lytic enzymes and HSAF/WAP-8294A2 biosynthesis, as well as several contact-dependent machineries, including type 2/4/6 secretion systems. Additionally, a variety of hydrolytic enzymes, particularly extracellular enzymes, were found in the L. capsici CK09 genome and predicted to improve its adaption in soil. Furthermore, several systems, including type 4 pili, type 3 secretion system and polysaccharide biosynthesis, can provide a selective advantage to L. capsici CK09, enabling the species to live on the surface in soil. The expression of these genes was then confirmed via transcriptomic analysis, indicating the activities of these genes. Collectively, our research provides a comprehensive understanding of the biocontrol potential and soil adaption of L. capsici CK09 and implies the potential of this strain for application in the future.

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Evaluation of plant protection efficacy in field conditions and side effects ofLysobacter capsiciAZ78, a biocontrol agent ofPlasmopara viticola

Cited by 8 publications

References 24 publications

Characterization of a Disease-Suppressive Isolate of Lysobacter enzymogenes with Broad Antagonistic Activity against Bacterial, Oomycetal and Fungal Pathogens in Different Crops

Characterization of a Disease-Suppressive Isolate of Lysobacter enzymogenes with Broad Antagonistic Activity against Bacterial, Oomycetal and Fungal Pathogens in Different Crops

THE USE OF BIOPREPARATIONS WITH BACTERIA AND FUNGI TO CONTROL DOWNY MILDEW (Plasmopara vitícola) AND REDUCE CHEMICAL TREATMENTS

Genetic Basis and Expression Pattern Indicate the Biocontrol Potential and Soil Adaption of Lysobacter capsici CK09

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