Evaluation of pilot-scale microencapsulation of probiotics and product effect on broilers1

Abstract

This study was conducted to evaluate the pilot-scale production of microencapsulated in a 500-L fermenter using emulsion and gelation and to assess the effect of the products on the growth performance, antioxidant activity, immune function, and cecal microbiota in Arbor Acres broilers. A total of seven hundred 1-d-old male Arbor Acres broilers were randomly assigned to 7 dietary treatments with 5 replicate pens per treatment and 20 broilers per pen. The dietary treatments were as follows: 1) basal diet (CON), 2) basal diet containing 0.1% Aureomycin (ANT), 3) basal diet containing unencapsulated at a dose of 1 × 10 cfu/kg of feed (P1), 4) basal diet containing unencapsulated at a dose of 1 × 10 cfu/kg of feed (P2), 5) basal diet containing 0.01% empty microcapsules (CAP), 6) basal diet containing microencapsulated at a dose of 1 × 10 cfu/kg of feed (CAPP1), and 7) basal diet containing microencapsulated at a dose of 1 × 10 cfu/kg of feed (CAPP2). The feeding experiment included 2 phases: the starter phase from d 1 to 21 and the grower phase from d 22 to 42. The results showed that a 500-L fermenter could produce 20.73 ± 4.05 kg of microcapsules with an approximate diameter of 549 μm. The feeding experiment showed that ADG of broilers in CAPP1 was significantly ( < 0.05) greater than that in CON and CAP throughout the feeding period, whereas the ratio of feed to gain (G:F) was significantly ( < 0.05) lower. Broilers in P1, P2, CAPP1, and CAPP2 had significantly ( < 0.05) greater levels of total superoxide dismutase, catalase, IgG, and cluster of differentiation 3 than those in CON. Furthermore, broilers in CAPP1 had significantly ( < 0.05) greater richness and diversity of intestinal microorganisms, particularly of , than those in all other dietary treatments. In summary, our results indicate that large-scale microencapsulation of microbial cells can be achieved using emulsion and initial gelation and that the dietary administration of microencapsulated can significantly enhance the growth performance, immune function, cecum microbial community, and overall health of broilers.

Growth and survival of microencapsulated probiotics prepared by emulsion and internal gelation

“…Two tested probiotic strains, including S. boulardii (CGMCC No. 10381) (Zhang et al 2015a, b) and E. faecium (CGMCC No. 2516) (Zhang et al 2015a, b), were cultured under microencapsulated and free non-encapsulated conditions.…”

Maximum levels of cross‐contamination for 24 antimicrobial active substances in non‐target feed. Part 12: Tetracyclines: tetracycline, chlortetracycline, oxytetracycline, and doxycycline

“…In the study of Zhang et al. ( 2015 ) a total of 700 one‐day‐old Arbor Acres chickens for fattening were allocated to seven dietary treatments and distributed in five pens per treatment, in groups of 20 birds per each pen. Two basal diets based on maize and soybean meal (starter days 1−21 and grower days 22−42) were either not supplemented (control) or supplemented with different treatments.…”

“…The two studies in laying hens showed that dietary chlortetracycline supplementation did not affect laying performance of hens (Uuganbatar et al., 2005, 500 mg chlortetracycline/kg feed; Zhang et al., 2015 , 1,000 mg chlortetracycline/kg feed).…”

Ácidos hidroxicinámicos en producción animal: farmacocinética, farmacodinamia y sus efectos como promotor de crecimiento. Revisión

“…Cabe indicar que esta estrategia de encapsulación se ha venido utilizado con otras moléculas y se han encontrado resultados eficientes; una de ellas es el zinc, donde a dosis de 100 ppm con una cubierta lipídica del 10 %, se pueden mitigar los síntomas de colibacilosis en cerdos destetados (96) ; además, diferentes estudios han demostrado que el uso de cultivos probióticos en forma protegida es una estrategia viable para mejorar la digestibilidad y absorción de nutrientes, mejorar el sistema inmunológico y prevenir infecciones en especies tanto rumiantes como monogástricos (97,98,99) . Por lo tanto, el diseño de sistemas de encapsulación de AH puede ser una estrategia cercana a implementarse con fines zootécnicos y con lo que podrá esclarecerse la ruta de acción y los efectos que estos compuestos pueden ocasionar.…”