Evaluation of phytochemicals, antioxidants, and antidiabetic efficacy of various solvent fractions of Gynura procumbens (Lour.) Merr

Sathiyaseelan, Anbazhagan; Park, Seon Ju; Saravanakumar, Kandasamy; Mariadoss, Arokia Vijay Anand; Wang, Myeong‐Hyeon

doi:10.1016/j.procbio.2021.08.028

Cited by 21 publications

(12 citation statements)

References 53 publications

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“…The antioxidant activity of bacterial strains was determined by DPPH and ABTS radical scavenging assay [ 32 , 76 ]. For 2,2-diphenyl-1-picrylhydrazyl (DPPH) (Sigma-Aldrich, St. Louis, MO, USA) assay, 100 µL of bacterial suspension (1 × 10 9 CFU/mL) was mixed with DPPH (100 µM) solution, incubated for 15 min in dark condition, the pellet removed by centrifugation, and the supernatant measured at 517 nm by UV–vis spectrophotometer.…”

Section: Methodsmentioning

confidence: 99%

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Antioxidant and Antibacterial Effects of Potential Probiotics Isolated from Korean Fermented Foods

Sathiyaseelan

Saravanakumar

Han

et al. 2022

IJMS

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A total of sixteen bacterial strains were isolated and identified from the fourteen types of Korean fermented foods that were evaluated for their in vitro probiotic potentials. The results showed the highest survivability for Bacillus sp. compared to Lactobacillus sp. in simulated gastric pH, and it was found to be maximum for B. inaquosorum KNUAS016 (8.25 ± 0.08 log10 CFU/mL) and minimum for L. sakei KNUAS019 (0.8 ± 0.02 log10 CFU/mL) at 3 h of incubation. Furthermore, B. inaquosorum KNUAS016 and L. brevis KNUAS017 also had the highest survival rates of 6.86 ± 0.02 and 5.37 ± 0.01 log10 CFU/mL, respectively, in a simulated intestinal fluid condition at 4 h of incubation. The percentage of autoaggregation at 6 h for L. sakei KNUAS019 (66.55 ± 0.33%), B. tequilensis KNUAS015 (64.56 ± 0.14%), and B. inaquosorum KNUAS016 (61.63 ± 0.19%) was >60%, whereas it was lower for L. brevis KNUAS017 (29.98 ± 0.09%). Additionally, B. subtilis KNUAS003 showed higher coaggregation at 63.84 ± 0.19% while B. proteolyticus KNUAS001 found at 30.02 ± 0.33%. Among them, Lactobacillus sp. showed the best non-hemolytic activity. The highest DPPH and ABTS radical scavenging activity was observed in L. sakei KNUAS019 (58.25% and 71.88%). The cell-free supernatant of Lactobacillus sp. considerably inhibited pathogenic growth, while the cell-free supernatant of Bacillus sp. was moderately inhibited when incubated for 24 h. However, the overall results found that B. subtilis KNUAS003, B. proteolyticus KNUAS012, L. brevis KNUAS017, L. graminis KNUAS018, and L. sakei KNUAS019 were recognized as potential probiotics through different functional and toxicity assessments.

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Section: Methodsmentioning

confidence: 99%

“…ABTS + scavenging ability was determined by measuring the absorbance of 734 nm. The percentage of DPPH and ABTS + scavenging activity was determined according to an earlier report [ 76 ].…”

Section: Methodsmentioning

confidence: 99%

Antioxidant and Antibacterial Effects of Potential Probiotics Isolated from Korean Fermented Foods

Sathiyaseelan

Saravanakumar

Han

et al. 2022

IJMS

Self Cite

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“…Next, the components of the obtained phenolic extracts were separated by liquid-liquid extraction between ethyl acetate and water. Ethyl acetate was selected for the fractionation procedure because previous work showed that the ethyl acetate fraction had a higher concentration of phenolic compounds and inhibited the activity of amylase and glucosidase more effectively compared to the hexane or methanol fraction [ 32 , 33 , 34 ].…”

Section: Resultsmentioning

confidence: 99%

In Vitro Inhibitory Effects of Viburnum opulus Bark and Flower Extracts on Digestion of Potato Starch and Carbohydrate Hydrolases Activity

et al. 2022

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One of the effective treatments for diabetes is to reduce and delay the absorption of glucose by inhibition of α-amylase and α-glucosidase in the digestive tract. Currently, there is a great interest in natural inhibitors from various part of plants. In the present study, the phenolic compounds composition of V. opulus bark and flower, and their inhibitory effects on in vitro potato starch digestion as well as on α-amylase and α-glucosidase, have been studied. Bark and flower phenolic extracts reduced the amount of glucose released from potato starch during tree-stage simulated digestion, with IC50 value equal to 87.77 µg/mL and 148.87 µg/mL, respectively. Phenolic bark extract showed 34.9% and 38.4% more potent inhibitory activity against α-amylase and α-glucosidase, respectively, but the activity of plant extracts was lower than that of acarbose. Chlorogenic acid (27.26% of total phenolics) and (+)-catechin (30.48% of total phenolics) were the most prominent phenolics in the flower and bark extracts, respectively. Procyanidins may be responsible for the strongest V. opulus bark inhibitory activity against α-amylase, while (+)-catechin relative to α-glucosidase. This preliminary study provides the basis of further examination of the suitability of V. opulus bark compounds as components of nutraceuticals and functional foods with antidiabetic activity.

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“…The compounds with the highest activity in G. procumbens were kaempferol, quercitin, caffeoyl-O-hexoside caffeoylquinic acid, coumaroyl-O-hexoside and coumaroylquinic acid. Bioinformatics studies have shown strong molecular interactions between natural compounds and digestive enzymes, thus underlining the value of studying these compounds [ 73 ].…”

Section: Plants Involved In Diabetes Mellitus Managementmentioning

confidence: 99%

“…Sathiyaseelan et al evaluated the antidiabetic effect of phytochemicals from Gynura procumbens methanolic extract and its various solvent fractions on α-amylase and α-glucosidase receptors. They also predicted the interaction of the main compounds identified in G. procumbens extract with porcine pancreatic α-amylase and α-glucosidase, using a molecular docking approach ( Table 1 ) [ 73 ].…”

Section: Molecular Docking and Molecular Dynamics Predicted Anti-diabetic Activitymentioning

confidence: 99%

Advanced Bioinformatics Tools in the Pharmacokinetic Profiles of Natural and Synthetic Compounds with Anti-Diabetic Activity

et al. 2021

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Diabetes represents a major health problem, involving a severe imbalance of blood sugar levels, which can disturb the nerves, eyes, kidneys, and other organs. Diabes management involves several synthetic drugs focused on improving insulin sensitivity, increasing insulin production, and decreasing blood glucose levels, but with unclear molecular mechanisms and severe side effects. Natural chemicals extracted from several plants such as Gymnema sylvestre, Momordica charantia or Ophiopogon planiscapus Niger have aroused great interest for their anti-diabetes activity, but also their hypolipidemic and anti-obesity activity. Here, we focused on the anti-diabetic activity of a few natural and synthetic compounds, in correlation with their pharmacokinetic/pharmacodynamic profiles, especially with their blood-brain barrier (BBB) permeability. We reviewed studies that used bioinformatics methods such as predicted BBB, molecular docking, molecular dynamics and quantitative structure-activity relationship (QSAR) to elucidate the proper action mechanisms of antidiabetic compounds. Currently, it is evident that BBB damage plays a significant role in diabetes disorders, but the molecular mechanisms are not clear. Here, we presented the efficacy of natural (gymnemic acids, quercetin, resveratrol) and synthetic (TAK-242, propofol, or APX3330) compounds in reducing diabetes symptoms and improving BBB dysfunctions. Bioinformatics tools can be helpful in the quest for chemical compounds with effective anti-diabetic activity that can enhance the druggability of molecular targets and provide a deeper understanding of diabetes mechanisms.

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Evaluation of phytochemicals, antioxidants, and antidiabetic efficacy of various solvent fractions of Gynura procumbens (Lour.) Merr

Cited by 21 publications

References 53 publications

Antioxidant and Antibacterial Effects of Potential Probiotics Isolated from Korean Fermented Foods

Antioxidant and Antibacterial Effects of Potential Probiotics Isolated from Korean Fermented Foods

In Vitro Inhibitory Effects of Viburnum opulus Bark and Flower Extracts on Digestion of Potato Starch and Carbohydrate Hydrolases Activity

Advanced Bioinformatics Tools in the Pharmacokinetic Profiles of Natural and Synthetic Compounds with Anti-Diabetic Activity

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